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Human Immunodeficiency Virus 1 (HIV-1) PhenoSense GT® Plus Integrase (Monogram® Phenotype + Genotype)

Test Number 551920
Test number copied
CPT 87900; 87901; 87903; 87904(x16); 87906
Synonyms
  • HIV Resistance
  • PhenoSense GT® Plus Integrase

Test Details

Methodology

Phenotype: polymerase chain reaction (PCR) amplification and viral culture; Genotype: polymerase chain reaction (PCR) and DNA sequencing. PhenoSense® and PhenoSense GT® are trademarks of Monogram Biosciences®.

Result Turnaround Time

19 - 23 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

PhenoSense GT® Plus Integrase

Test Includes

This assay is a combined assay in which HIV resistance phenotype and HIV resistance genotype is performed.

Use

Determines viral phenotype and genotype resistance to four classes of commonly prescribed antiretroviral drugs: nucleoside reverse transcriptase inhibitors (NRTI), nonnucleoside reverse transcriptase inhibitors (NNRTI), protease inhibitors (PI), and integrase inhibitors (INI).

Special Instructions

A separate frozen specimen is required for each frozen test ordered.

Limitations

This procedure should be used for patients with documented HIV-1 infection and viral loads ≥500 copies/mL. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and this procedure will be billed at the account's prevailing fee.

References

Boyd SD, Maldarelli F, Sereti I, et al. Transmitted raltegravir resistance in an HIV-1 CRF_AG-infected patient. Antivir Ther. 2011; 16(2):257-261. 21447876
Cohen C, Hunt S, Sension M, et al. Phenotypic resistance testing significantly improves response to therapy (Tx): A randomized trial (VIRA3001), San Francisco, Calif: Seventh Conference on Retroviruses and Opportunistic Infections. 2000. Abstract 237.
Durant J, Clevenbergh P, Halfon P, et al. Drug-resistance genotyping in HIV-1 therapy: The VIRADAPT randomized controlled trial. Lancet. 1999 Jun 26; 353(9171):2195-2199. Erratum: Lancet. 1999; 354(9184):1128. 10392984
Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Department of Health and Human Services. Washington, DC: Department of Health and Human Services. January 28, 2000. Available at http://aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Accessed February 10, 2012.
Parkin N, Chappey C, Maroldo L, Bates M, Hellmann NS, Petropoulos CJ. Phenotypic and genotypic HIV-1 drug resistance assays provide complementary information. J Acquir Immune Defic Syndr. 2002 Oct 1; 31(2):128-136. 12394790
Walworth CM, Ward D, Napolitano L, et al. Optimized antiretroviral drug selection achieves rapid and sustained supression of viral replication, despite transmission of HIV-1 exhibiting resistance to 5-drug classes. International Workshop on HIV and Hepatitis Virus. June 5-9, 2012, Stiges, Spain.
Young B, Fransen S, Greenberg KS, et al. Transmission of integrase strand-transfer inhibitor multidrug-resistant HIV-1: Case report and response to raltegravir-containing antiretroviral therapy. Antivir Ther. 2011; 16(2):253-256. 21447875

Specimen Requirements

Specimen

Plasma, frozen

Volume

5 mL

Collection Instructions

Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more polypropylene screw-capped tube(s) (not "pop-top" or "snap-cap") and freeze. Ship specimen frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Causes for Rejection

PPT™ not centrifuged; quantity not sufficient for analysis; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles; specimen received in "pop-top" or "snap-cap" tube

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551920 PhenoSenseGT(R) plus Integrase 551921 PSGT plus Integrase PDF 51969-4
551920 PhenoSenseGT(R) plus Integrase 551922 PSGT+IN Net Assessment 83326-9
551920 PhenoSenseGT(R) plus Integrase 551923 PSGT+IN Phenotype Results 49665-3
551920 PhenoSenseGT(R) plus Integrase 551924 PSGT+IN Genotype Results 80689-3
551920 PhenoSenseGT(R) plus Integrase 551925 PSGT+IN Interpretation 77202-0
Order Code551920
Order Code NamePhenoSenseGT(R) plus Integrase
Order Loinc
Result Code551921
Result Code NamePSGT plus Integrase PDF
UofM
Result LOINC51969-4
Order Code551920
Order Code NamePhenoSenseGT(R) plus Integrase
Order Loinc
Result Code551922
Result Code NamePSGT+IN Net Assessment
UofM
Result LOINC83326-9
Order Code551920
Order Code NamePhenoSenseGT(R) plus Integrase
Order Loinc
Result Code551923
Result Code NamePSGT+IN Phenotype Results
UofM
Result LOINC49665-3
Order Code551920
Order Code NamePhenoSenseGT(R) plus Integrase
Order Loinc
Result Code551924
Result Code NamePSGT+IN Genotype Results
UofM
Result LOINC80689-3
Order Code551920
Order Code NamePhenoSenseGT(R) plus Integrase
Order Loinc
Result Code551925
Result Code NamePSGT+IN Interpretation
UofM
Result LOINC77202-0
Reflex Table for PSGT+IN Net Assessment
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550504 Quant, RNA PCR 550497 HIV-1 RNA by PCR copies/mL 20447-9
Reflex 1
Order Code550504
Order NameQuant, RNA PCR
Result Code550497
Result NameHIV-1 RNA by PCR
UofMcopies/mL
Result LOINC20447-9
Reflex Table for PSGT+IN Net Assessment
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550504 Quant, RNA PCR 550498 log10 HIV-1 RNA log10copy/mL 29541-0
Reflex 1
Order Code550504
Order NameQuant, RNA PCR
Result Code550498
Result Namelog10 HIV-1 RNA
UofMlog10copy/mL
Result LOINC29541-0