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Human Immunodeficiency Virus 1 (HIV-1) GenoSure PRIme®

Test Number 551700
Test number copied
CPT Call 800-222-7566, ext 6-8400, for coding.
Synonyms
  • GenoSure PRime®
  • HIV-1 Genotype
  • POL Genotype
  • Resistance Testing
  • Retroviral Genotype

Test Details

Methodology

Polymerase chain reaction (PCR) amplification and DNA sequencing.

Result Turnaround Time

11 - 14 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

A Comprehensive Resistance Profile in a Single Test

GenoSure PRIme®: Detection of HIV-1 Protease, Reverse Transcriptase, and Integrase Inhibitor Resistance

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

Supporting Individualized HIV Treatment Decisions

Use

Detect resistance of HIV-1 to protease, reverse transcriptase, and integrase inhibitors.

Special Instructions

Submit a separate frozen specimen for each test requested.

Limitations

This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account's prevailing fee.

References

Boyd SD, Maldarelli F, Sereti I, et al. Transmitted raltegravir resistance in an HIV-1 CRF_AG-infected patient. Antivir Ther. 2011; 16(2):257-261. 21447876
Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Department of Health and Human Services. Washington, DC: Department of Health and Human Services. January 28, 2000. Available at http://aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Accessed February 10, 2012.
Thompson MA, Aberg JA, Cahn P, et al. Antiretroviral treatment of adult HIV infection: 2010 recommendations of the International AIDS Society-USA panel. JAMA. 2010 Jul 21, 304(3):321-333. 20639566
Young B, Fransen S, Greenberg K, et al. Transmission of integrase strand-transfer inhibitor multidrug-resistant HIV-1: Case report and response to raltegravir-containing antiretroviral therapy. Antivir Ther. 2011; 16(2):253-256. 21447875

Specimen Requirements

Specimen

Plasma, frozen

Volume

5 mL

Container

Lavender-top (EDTA) or plasma preparation tubes (PPT™)

Collection Instructions

Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or Pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at - 20° C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Stability Requirements

Temperature

Period

Frozen

2 years

Storage Instructions

Freeze for shipment or long-term storage.

Causes for Rejection

PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551700 GenoSure PRIme(R) 80689-3 551703 PDF 51969-4
551700 GenoSure PRIme(R) 80689-3 551701 HIV GenoSure PRIme(R) 93047-9
551700 GenoSure PRIme(R) 80689-3 551702 HIV GenoSure PRIme(R) 80689-3
551700 GenoSure PRIme(R) 80689-3 551706 HIV GenoSure PRIme(R) Interp 62364-5
Order Code551700
Order Code NameGenoSure PRIme(R)
Order Loinc80689-3
Result Code551703
Result Code NamePDF
UofM
Result LOINC51969-4
Order Code551700
Order Code NameGenoSure PRIme(R)
Order Loinc80689-3
Result Code551701
Result Code NameHIV GenoSure PRIme(R)
UofM
Result LOINC93047-9
Order Code551700
Order Code NameGenoSure PRIme(R)
Order Loinc80689-3
Result Code551702
Result Code NameHIV GenoSure PRIme(R)
UofM
Result LOINC80689-3
Order Code551700
Order Code NameGenoSure PRIme(R)
Order Loinc80689-3
Result Code551706
Result Code NameHIV GenoSure PRIme(R) Interp
UofM
Result LOINC62364-5
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551704 GenoSure PRIme(R) 551704 GenoSure PRIme(R) N/A
Reflex 1
Order Code551704
Order NameGenoSure PRIme(R)
Result Code551704
Result NameGenoSure PRIme(R)
UofM
Result LOINCN/A
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550503 Quant, RNA PCR 550494 HIV-1 RNA by PCR copies/mL 20447-9
Reflex 1
Order Code550503
Order NameQuant, RNA PCR
Result Code550494
Result NameHIV-1 RNA by PCR
UofMcopies/mL
Result LOINC20447-9
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550503 Quant, RNA PCR 550495 log10 HIV-1 RNA log10copy/mL 29541-0
Reflex 1
Order Code550503
Order NameQuant, RNA PCR
Result Code550495
Result Namelog10 HIV-1 RNA
UofMlog10copy/mL
Result LOINC29541-0
Reflex Table for HIV GenoSure PRIme(R)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551707 GenoSure PRIme(R) Interp 551707 GenoSure PRIme(R) Interp N/A
Reflex 1
Order Code551707
Order NameGenoSure PRIme(R) Interp
Result Code551707
Result NameGenoSure PRIme(R) Interp
UofM
Result LOINCN/A