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Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to Trofile®
CPT:
87536
Synonyms
- HIV-1 Plasma Viremia
- HIV-1 RNA by Real-time (RT) PCR, Quantitative
- Reflex to Trofile®
- Viral Load
Expected Turnaround Time
17 - 25 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
For more information, please view the literature below.
HIV-1 Lifecycle, Drugs, and Susceptibility Assays
Specimen Requirements
Specimen
Plasma, frozen
Volume
6 mL
Minimum Volume
4 mL (Note: This volume does not allow for repeat testing.)
Container
Ship in two or more screw-cap polypropylene frozen transport tubes.
Collection
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to screw-cap tubes prior to freezing to avoid false elevation of low viral load samples. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze. Separate frozen sample required for Trofile® portion of the test. Frozen sample is preferred for the viral load assay but is also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™
Test Details
Use
Detect and quantitate HIV-1 in plasma with reflex to phenotype (at additional charge) if viral load is >1000 copies/mL.
Limitations
Assay range: 20−10,000,000 copies/mL
Methodology
Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550390 | RNA,PCR(Graph) rfx/Trofile(R) | 75622-1 | 550445 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| 550390 | RNA,PCR(Graph) rfx/Trofile(R) | 75622-1 | 550446 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| 550390 | RNA,PCR(Graph) rfx/Trofile(R) | 75622-1 | 550465 | Trofile(R) | N/A | |
| 550390 | RNA,PCR(Graph) rfx/Trofile(R) | 75622-1 | 511958 | 80563-0 |
| Reflex Table for Trofile(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551156 | Reflex to Trofile(R) | 829632 | Trofile(R) PDF | 51969-4 | |
| Reflex Table for Trofile(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551156 | Reflex to Trofile(R) | 829666 | Tropotype: | 50790-5 | |
| Reflex Table for Trofile(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551156 | Reflex to Trofile(R) | 829633 | Trofile(R) | 57182-8 | |
| Reflex Table for Trofile(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551156 | Reflex to Trofile(R) | 829634 | Trofile(R) Interpretation | 77202-0 | |
| Reflex Table for Trofile(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551156 | Reflex to Trofile(R) | 829635 | Trofile(R) Methodology | 49549-9 | |
| Reflex Table for Trofile(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551156 | Reflex to Trofile(R) | 829636 | Trofile(R) References | 75608-0 | |
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CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf