Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical)

CPT: 87536
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time PCR, Quantitative

Special Instructions

Submit the patient's Social Security number for serial monitoring. To order this test without the serial monitor service, use test 550430.


Expected Turnaround Time

3 - 5 days



Specimen Requirements


Specimen

Plasma


Volume

3.5 mL


Minimum Volume

1.1 mL (Note: This volume does not allow for repeat testing.)


Container

Lavender-top (EDTA) tube or plasma preparation tube (PPT™)


Collection

Centrifuge sample within 24 hours of collection. Transfer plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.


Storage Instructions

Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for six days. Stable frozen for six weeks.


Causes for Rejection

Incorrect anticoagulant; PPT™ frozen and/or not centrifuged


Test Details


Use

Detect and quantitate HIV-1 in plasma


Limitations

Assay range: 20−10,000,000 copies/mL


Methodology

Cobas® HIV


References

Wirden M, Larrouy L, Mahjoub N, et al. Multicenter comparison of the new Cobas 6800 system with Cobas Ampliprep/Cobas TaqMan and Abbott RealTime for the quantification of HIV, HBV and HCV viral load. J Clin Virol. 2017 Nov;96:49-53.28950186

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550420 RNA, Real Time PCR (Graph) 20447-9 550413 HIV-1 RNA by PCR copies/mL 20447-9
550420 RNA, Real Time PCR (Graph) 20447-9 550414 log10 HIV-1 RNA log10copy/mL 29541-0
550420 RNA, Real Time PCR (Graph) 20447-9 511958 PDF 80563-0

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