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Human Immunodeficiency Virus 1 (HIV-1) DNA Sequencing Protease−Reverse Transcriptase

Test Number 551730
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Synonyms
  • Cell-associated Viral DNA
  • HIV-1 Genotype
  • Resistance Analysis

Test Details

Methodology

Polymerase chain reaction (PCR) amplification and next generation DNA sequencing

Result Turnaround Time

10 - 14 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

Coreceptor Tropism Assays

GenoSure Archive℠: Cell-associated Viral DNA Assay for HIV-1 Protease, Reverse Transcriptase, and Integrase Inhibitors

GenoSure Archive℠: HIV-1 Next Generation DNA Sequencing Assay for Suppression Management

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

Use

Detect resistance of HIV-1 to protease and reverse transcriptase inhibitors in cell-associated viral DNA.

Limitations

This procedure should be used for patients with documented HIV-1 infection and undetectable viral load or low level viremia.

Specimen Requirements

Specimen

Whole blood, frozen

Volume

4 mL

Container

Lavender-top (EDTA) tube

Collection Instructions

Collect specimen in lavender-top (EDTA) tube. Do not centrifuge. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Causes for Rejection

Quantity not sufficient for analysis; heparinized plasma; nonfrozen specimen

References

Günthard HF, Aberg JA, Eron JJ, et al. Antiretroviral treatment of adult HIV infection: 2014 recommendations of the International Antiviral Society-USA Panel. JAMA. 2014 Jul 23-30; 312(4):410-425. 25038359
Panel on Antiretroviral Guidelines for Adults and Adolescents—A Working Group of the Office of AIDS Research Advisory Council (OARAC). Guidelines for the Use of Antiretroviral Agents in HIV-1-Infected Adults and Adolescents. Washington, DC: Department of Health and Human Services; May 1, 2014.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551730 DNA Sequencing PR-RT 80692-7 551733 PDF 80695-0
551730 DNA Sequencing PR-RT 80692-7 551731 HIV DNA Sequencing PR-RT N/A
551730 DNA Sequencing PR-RT 80692-7 551732 HIV DNA Sequencing PR-RT 80692-7
551730 DNA Sequencing PR-RT 80692-7 551729 HIV DNA Sequencing PRRT Interp 80694-3
Order Code551730
Order Code NameDNA Sequencing PR-RT
Order Loinc80692-7
Result Code551733
Result Code NamePDF
UofM
Result LOINC80695-0
Order Code551730
Order Code NameDNA Sequencing PR-RT
Order Loinc80692-7
Result Code551731
Result Code NameHIV DNA Sequencing PR-RT
UofM
Result LOINCN/A
Order Code551730
Order Code NameDNA Sequencing PR-RT
Order Loinc80692-7
Result Code551732
Result Code NameHIV DNA Sequencing PR-RT
UofM
Result LOINC80692-7
Order Code551730
Order Code NameDNA Sequencing PR-RT
Order Loinc80692-7
Result Code551729
Result Code NameHIV DNA Sequencing PRRT Interp
UofM
Result LOINC80694-3
Reflex Table for HIV DNA Sequencing PR-RT
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551734 HIVDNASeq PRRT 551734 HIVDNASeq PRRT N/A
Reflex 1
Order Code551734
Order NameHIVDNASeq PRRT
Result Code551734
Result NameHIVDNASeq PRRT
UofM
Result LOINCN/A
Reflex Table for HIV DNA Sequencing PR-RT
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551743 HIVDNASeq PRRT Interp 551743 HIVDNASeq PRRT Interp N/A
Reflex 1
Order Code551743
Order NameHIVDNASeq PRRT Interp
Result Code551743
Result NameHIVDNASeq PRRT Interp
UofM
Result LOINCN/A