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Human Immunodeficiency Virus 1 (HIV-1) GenoSure® MG Genotyping
CPT:
Call 800-222-7566, ext 6-8400, for coding.
Synonyms
- HIV-1 Genotype
- Resistance Analysis
- Retroviral Genotype
Special Instructions
A separate frozen specimen is required for each frozen test ordered.
Expected Turnaround Time
12 - 15 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
For more information, please view the literature below.
Assays to Support HIV Treatment Decisions
HIV-1 Lifecycle, Drugs, and Susceptibility Assays
Human Immunodeficiency Virus 1/O/2 Cascade: Fourth-generation Antigen/Antibody Detection
Specimen Requirements
Specimen
Plasma, frozen
Volume
5 mL
Container
Lavender-top (EDTA) or plasma preparation tube (PPT™)
Collection
Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). Snap-cap or pop-top tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20°C. Do not place samples on dry ice until completely frozen. Specimens collected in PPT™ need to be centrifuged within six hours and frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Causes for Rejection
PPT™ not centrifuged; insufficient volume; heparinized plasma; nonfrozen specimens; specimens exposed to repeated freeze/thaw cycles
Test Details
Use
Detect resistance in HIV-1 to antiretroviral drugs targeted at the reverse transcriptase (nucleoside and nonnucleoside inhibitors) and protease.
Limitations
This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account's prevailing fee.
Methodology
Polymerase chain reaction (PCR) amplification and DNA sequencing
References
Hirsch MS, Brun-Vézinet F, D'Aquila RT, et al. Antiretroviral drug resistance testing in adult HIV-1 infection. Recommendations of an International AIDS Society-USA panel. JAMA. 2000; 283(18):2417-2426. 10815085
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 551697 | GenoSure(R) MG | 53798-5 | 551693 | 49573-9 | ||
| 551697 | GenoSure(R) MG | 53798-5 | 551656 | HIV GenoSure(R) | 93047-9 | |
| 551697 | GenoSure(R) MG | 53798-5 | 551657 | HIV GenoSure(R) | 53798-5 |
| Reflex Table for HIV GenoSure(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551318 | Quant, RNA PCR | 551311 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| Reflex Table for HIV GenoSure(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551318 | Quant, RNA PCR | 551312 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| Reflex Table for HIV GenoSure(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551624 | HIV GenoSure(R) MG | 551624 | HIV GenoSure(R) MG | 49573-9 | |
| Reflex Table for HIV GenoSure(R) | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551619 | HIV GenoSure(R) MG Interp | 551619 | HIV GenoSure(R) MG Interp | 49573-9 | |
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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf