Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to HIV-1 PhenoSense GT® Plus Integrase

TEST: 550711
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CPT: 87536
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time PCR, Quantitative
  • Reflex to PhenoSense GT® Plus Integrase
  • Viral Load

Special Instructions

A separate frozen specimen is required for the PhenoSense GT® Plus Integrase portion of the test. A frozen sample is preferred for the viral load assay, but it is also stable at room temperature for one day or refrigerated for five days. Freeze specimen for long-term storage.

Expected Turnaround Time

24 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide

HIV-1 Lifecycle, Drugs, and Susceptibility Assays

    Specimen Requirements

    Specimen

    Plasma, frozen

    Volume

    6 mL

    Minimum Volume

    4 mL (Note: This volume does not allow for repeat testing.)

    Container

    Ship in two or more screw-cap polypropylene frozen transport tubes.

    Collection

    Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship frozen (preferred). Do not ship frozen in PPT™. If PPT™ is used to collect plasma, specimen must be transferred to a screw-cap tube prior to freezing to avoid false elevation of low viral load samples. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

    Storage Instructions

    Freeze. Separate frozen sample required for PhenoSense GT® portion of the test. A frozen sample is preferred for the viral load assay, but it is also stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.

    Causes for Rejection

    Hemolysis; green-top (heparin) tube; specimen received in "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; frozen PPT™

    Test Details

    Use

    Detect and quantitate HIV-1 in plasma with reflex to PhenoSense GT® Plus Integrase (at additional charge) if viral load is >500 copies/mL.

    Limitations

    Assay range: 20−10,000,000 copies/mL

    Methodology

    Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0

    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    550711 RNA PCR(Graph)rfx/PSGT+IN 550445 HIV-1 RNA by PCR copies/mL 20447-9
    550711 RNA PCR(Graph)rfx/PSGT+IN 550446 log10 HIV-1 RNA log10copy/mL 29541-0
    550711 RNA PCR(Graph)rfx/PSGT+IN 550626 PhenoSenseGT(R) plus Integrase N/A
    550711 RNA PCR(Graph)rfx/PSGT+IN 511958 PDF 80563-0
    Reflex Table for PhenoSenseGT(R) plus Integrase
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551930 Reflex to PSGT plus Integrase 551921 PSGT plus Integrase PDF 51969-4
    Reflex Table for PhenoSenseGT(R) plus Integrase
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551930 Reflex to PSGT plus Integrase 551922 PSGT+IN Net Assessment 83326-9
    Reflex Table for PhenoSenseGT(R) plus Integrase
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551930 Reflex to PSGT plus Integrase 551923 PSGT+IN Phenotype Results 49665-3
    Reflex Table for PhenoSenseGT(R) plus Integrase
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551930 Reflex to PSGT plus Integrase 551924 PSGT+IN Genotype Results 80689-3
    Reflex Table for PhenoSenseGT(R) plus Integrase
    Order Code Order Name Result Code Result Name UofM Result LOINC
    Reflex 1 551930 Reflex to PSGT plus Integrase 551925 PSGT+IN Interpretation 77202-0

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