Test Details
Methodology
Short-term cell culture; synchronization; chromosome harvest; G-banding; analysis (20 metaphases) and karyotyping; long-term β-mitogen cultures in some B-cell leukemias
Result Turnaround Time
7 - 10 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Short-term and, in some cases, long-term β-mitogen culturing of specimens, chromosome preparation, and analysis of 20 metaphases; multiple karyotypes prepared, including all abnormal clones
Use
Karyotyping, physical localization of copy number changes, and mapping of breakpoints involved in translocations.
Special Instructions
Include tentative diagnosis/treatment on the test request form. This is necessary for proper culturing and result interpretation. If plasma-enriched Multiple Myeloma (MM) Profile, FISH [510325] is ordered concurrently and sample is not sufficient for both tests, FISH will be prioritized. Please direct any questions regarding this test to customer service at 800-345-4363.
Limitations
Use of lithium heparin (or EDTA) will inhibit growth of cell culture.
Specimen Requirements
Specimen
Bone marrow, blood (>4% blasts required − see Collection), lymph node, spleen, bone core, or effusion
Volume
2 mL bone marrow, 3 mL peripheral blood, or 0.5 to 1 cm³ tissue biopsy
Container
Bone marrow or peripheral blood in pediatric sodium heparin green-top tube; lymph node, spleen, bone core, CSF, or effusion in lymph node transport bottle
Collection Instructions
The requirement of >4% blasts for blood does not apply to cases of CLL, hairy cell leukemia, and some cases of small-cell lymphocytic leukemia (SCLL) or lymphoma, which can be β-mitogen stimulated. Submit bone marrow or peripheral blood at room temperature using Leukemia/Lymphoma Specimen Transport Kit (supplied by LabCorp). Submit lymph node, spleen, or bone core at room temperature using Lymph Node Transport Kit (supplied by LabCorp). Effusions may be sent in small sterile containers. Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on the test request form.
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Hemolysis; gross contamination; non-neoplastic cells; clotted specimen; frozen specimen; fixed specimen; improper or too much anticoagulant
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 510999 | Chromosome, Leukemia/Lymphoma | 510678 | Specimen Type | 31208-2 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 510679 | Cells Counted | 62361-1 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 510680 | Cells Analyzed | 62360-3 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 510681 | Cells Karyotyped | 55199-4 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 510682 | GTG Band Resolution Achieved | 62358-7 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 510683 | Cytogenetic Result | 62356-1 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 510992 | Interpretation | 62365-2 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 510993 | Director Review: | 48672-0 | ||
| 510999 | Chromosome, Leukemia/Lymphoma | 512123 | 11502-2 | |||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510678 | |||||
| Result Code Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510679 | |||||
| Result Code Name | Cells Counted | |||||
| UofM | ||||||
| Result LOINC | 62361-1 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510680 | |||||
| Result Code Name | Cells Analyzed | |||||
| UofM | ||||||
| Result LOINC | 62360-3 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510681 | |||||
| Result Code Name | Cells Karyotyped | |||||
| UofM | ||||||
| Result LOINC | 55199-4 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510682 | |||||
| Result Code Name | GTG Band Resolution Achieved | |||||
| UofM | ||||||
| Result LOINC | 62358-7 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510683 | |||||
| Result Code Name | Cytogenetic Result | |||||
| UofM | ||||||
| Result LOINC | 62356-1 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510992 | |||||
| Result Code Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 62365-2 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 510993 | |||||
| Result Code Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 | |||||
| Order Code | 510999 | |||||
| Order Code Name | Chromosome, Leukemia/Lymphoma | |||||
| Order Loinc | ||||||
| Result Code | 512123 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 11502-2 |