Test Details
Methodology
16S gene sequencing; ancillary sequencing and/or biochemical testing as required
Result Turnaround Time
14 - 30 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
If MALDI-TOF based identification has not been performed, test code 183402 should be ordered.
Determine the identification of isolates (ie, bacterial, fungal, mycobacterial, etc) from another laboratory that has performed culture of the clinical specimen. These isolates may include strict aerobes, facultative anaerobes, anaerobes, yeast, filamentous fungi, mycobacteria, Nocardia, and/or actinomycetes.
16S gene sequencing will be the primary identification method. In some situations, additional ancillary testing (ie, biochemicals, alternative sequencing) may also need to be performed. Susceptibility testing may be requested for an additional charge. The client submitting the isolate for testing should provide LabCorp with clinical and testing information regarding test procedures already performed at the submitting laboratory in order to expedite testing upon receipt.
Special Instructions
This test first requires assessment of the kind of organism that will initiate a cascade reflex to the correct sequence primers. If reflex test is performed, additional charges/CPT code(s) may apply.
Limitations
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Specimen Requirements
Specimen
Pure culture isolate of bacteria, yeast, mold, Nocardia, or mycobacteria
Volume
Pure culture isolate (visible colonies should be present).
Minimum Volume
Viable colonies macroscopically visible.
Container
Bacterial swab transport inoculated with the isolate from a pure culture or agar slant in screw-cap container packed as an etiologic agent. Maintain a subculture at the submitting laboratory.
Storage Instructions
Maintain media at room temperature. Ensure that plated media has been securely sealed with parafilm.
Causes for Rejection
Isolate nonviable; unlabeled specimen or name discrepancy between specimen and request label; broken tube or plate; mixed culture
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 183285 | Organism ID by Sequencing | 183286 | Organism ID by Sequencing | 41852-5 | ||
| Order Code | 183285 | |||||
| Order Code Name | Organism ID by Sequencing | |||||
| Order Loinc | ||||||
| Result Code | 183286 | |||||
| Result Code Name | Organism ID by Sequencing | |||||
| UofM | ||||||
| Result LOINC | 41852-5 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 183269 | Aerobic Bacteria, ID by Seq. | 183270 | Aerobic Bacteria, ID by Seq. | 42803-7 | |
| Reflex 1 | ||||||
| Order Code | 183269 | |||||
| Order Name | Aerobic Bacteria, ID by Seq. | |||||
| Result Code | 183270 | |||||
| Result Name | Aerobic Bacteria, ID by Seq. | |||||
| UofM | ||||||
| Result LOINC | 42803-7 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 183301 | Anaerobic Bacteria, ID by Seq. | 183302 | Anaerobic Bacteria, ID by Seq. | 42803-7 | |
| Reflex 1 | ||||||
| Order Code | 183301 | |||||
| Order Name | Anaerobic Bacteria, ID by Seq. | |||||
| Result Code | 183302 | |||||
| Result Name | Anaerobic Bacteria, ID by Seq. | |||||
| UofM | ||||||
| Result LOINC | 42803-7 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 183277 | Nocardia, ID by Sequencing | 183278 | Nocardia, ID by Sequencing | 55096-2 | |
| Reflex 1 | ||||||
| Order Code | 183277 | |||||
| Order Name | Nocardia, ID by Sequencing | |||||
| Result Code | 183278 | |||||
| Result Name | Nocardia, ID by Sequencing | |||||
| UofM | ||||||
| Result LOINC | 55096-2 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 183293 | Mycobacteria, ID by Seq. | 183294 | Mycobacteria, ID by Seq. | 9825-1 | |
| Reflex 1 | ||||||
| Order Code | 183293 | |||||
| Order Name | Mycobacteria, ID by Seq. | |||||
| Result Code | 183294 | |||||
| Result Name | Mycobacteria, ID by Seq. | |||||
| UofM | ||||||
| Result LOINC | 9825-1 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 183319 | Yeast, ID by Sequencing | 183320 | Yeast, ID by Sequencing | 42804-5 | |
| Reflex 1 | ||||||
| Order Code | 183319 | |||||
| Order Name | Yeast, ID by Sequencing | |||||
| Result Code | 183320 | |||||
| Result Name | Yeast, ID by Sequencing | |||||
| UofM | ||||||
| Result LOINC | 42804-5 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 183327 | Mold, ID by Sequencing | 183328 | Mold, ID by Sequencing | 42804-5 | |
| Reflex 1 | ||||||
| Order Code | 183327 | |||||
| Order Name | Mold, ID by Sequencing | |||||
| Result Code | 183328 | |||||
| Result Name | Mold, ID by Sequencing | |||||
| UofM | ||||||
| Result LOINC | 42804-5 | |||||