Organism Identification by Matrix-assisted Laser Desorption/Ionization Time-of-Flight MS With Reflex to Sequence-based Identification

Test Number 183402

CPT Call client services.

Synonyms

MALDI-TOF Organism Identification

Test Details

Methodology

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is the primary identification method. Ancillary testing (ie, 16S gene sequencing, ITS gene sequencing, conventional biochemicals) will be employed as required.

Result Turnaround Time

5 - 15 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Use

Determine the identification of isolates (ie, bacterial, mycobacterial, etc) from another laboratory that has performed culture of the clinical specimen. These isolates may include strict aerobes, facultative anaerobes, anaerobes, yeast, mycobacteria, Nocardia and/or Actinomycetes.

Special Instructions

Susceptibility testing may be requested for an additional charge. The client submitting the isolate for testing should provide Labcorp with clinical and testing information regarding test procedures already performed at the submitting laboratory in order to expedite testing on receipt.

Broth medium containing a pure culture may be submitted for testing; however, additional time will be required in order to perform subculture to solid media prior to initiation of MALDI testing. Testing will reflex to sequencing when ID cannot be determined by MALDI-TOF.

Limitations

Broth medium containing a pure culture may be submitted for testing; however, additional time will be required in order to perform subculture to solid media prior to initiation of MALDI testing.

The technology is limited to what is claimed in the spectral database. If organism is not obtained, the test will reflex to identification by ITS gene sequencing.

Specimen Requirements

Specimen

Pure culture isolate of bacteria, yeast, filamentous fungi, AFB, Nocardia, or aerobic Actinomycetes

Volume

Pure culture (visible colonies should be present). Bacterial culture transport swab with media inoculated with the isolate from a pure culture or agar slant in a screw-cap container packed as an etiologic agent. Maintain a subculture at the submitting laboratory.

Minimum Volume

Viable colonies macroscopically visible on culture medium; one bacterial culture transport swab with media.

Container

Bacterial swab transport inoculated with the isolate from a pure culture or agar slant in screw-cap container packed as an etiologic agent. Maintain a subculture at the submitting laboratory.

Storage Instructions

Room temperature

Causes for Rejection

Isolate nonviable; unlabeled specimen or name discrepancy between the specimen and the name in the computer or on the test request form; isolate transported under inappropriate conditions; broken tube or plate; mixed culture; single specimen identifier

References

Dekker JP, Branda JA. MALDI-TOF mass spectrometry in the clinical microbiology laboratory. Clin Micro Newsletter. 2011 Jun 15; 33(12): 87-93.

Khot PD, Fisher MA. Mass spectrometry in the clinical microbiology laboratory, Part II: MALDI-TOF MS. Clin Micro Newsletter. 2012 Sep 15; 34(18): 143-147.

Lavigne J-P, Espinal P, Dunyach-Remy C, Messad N, Pantel A, Sotto A. Mass spectrometry: A revolution in clinical microbiology? Clin Chem Lab Med. 2013 Feb; 51(2):257-270. 23072853

Patel R. MALDI-TOF mass spectrometry: Transformative proteomics for clinical microbiology. Clin Chem. 2013 Feb; 59(2):340-342. 22665917

Verroken A, Janssens M, Berhin C, et al. Evaluation of matrix-assisted laser desorption ionization−time of flight mass spectrometry for identification of Nocardia species.J Clin Microbiol. 2010 Nov; 48(11):4015-4021. 20861335

El-Khéchine A, Couderc C, Flaudrops C, Raoult D, Drancourt M. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice. PLos One. 2011; 6(9):e24720. 21935444

LOINC® Map

Order Code	Order Code Name	Order Loinc	Result Code	Result Code Name	Result LOINC
183402	Organism ID by MALDI	76346-6	183403	Organism ID by MALDI	76346-6
Order Code	183402
Order Code Name	Organism ID by MALDI
Order Loinc	76346-6
Result Code	183403
Result Code Name	Organism ID by MALDI
UofM
Result LOINC	76346-6

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183404	Aerobic ID by MALDI	183404	Aerobic ID by MALDI	75756-7
Reflex 1
Order Code	183404
Order Name	Aerobic ID by MALDI
Result Code	183404
Result Name	Aerobic ID by MALDI
UofM
Result LOINC	75756-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183404	Aerobic ID by MALDI	183404	Aerobic ID by MALDI	75756-7
Reflex 1
Order Code	183404
Order Name	Aerobic ID by MALDI
Result Code	183404
Result Name	Aerobic ID by MALDI
UofM
Result LOINC	75756-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183404	Aerobic ID by MALDI	183404	Aerobic ID by MALDI	75756-7
Reflex 1
Order Code	183404
Order Name	Aerobic ID by MALDI
Result Code	183404
Result Name	Aerobic ID by MALDI
UofM
Result LOINC	75756-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183404	Aerobic ID by MALDI	183404	Aerobic ID by MALDI	75756-7
Reflex 1
Order Code	183404
Order Name	Aerobic ID by MALDI
Result Code	183404
Result Name	Aerobic ID by MALDI
UofM
Result LOINC	75756-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183404	Aerobic ID by MALDI	183404	Aerobic ID by MALDI	75756-7
Reflex 1
Order Code	183404
Order Name	Aerobic ID by MALDI
Result Code	183404
Result Name	Aerobic ID by MALDI
UofM
Result LOINC	75756-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183404	Aerobic ID by MALDI	183404	Aerobic ID by MALDI	75756-7
Reflex 1
Order Code	183404
Order Name	Aerobic ID by MALDI
Result Code	183404
Result Name	Aerobic ID by MALDI
UofM
Result LOINC	75756-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183405	Anaerobic ID by MALDI	183405	Anaerobic ID by MALDI	75756-7
Reflex 1
Order Code	183405
Order Name	Anaerobic ID by MALDI
Result Code	183405
Result Name	Anaerobic ID by MALDI
UofM
Result LOINC	75756-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183406	Nocardia ID by MALDI	183406	Nocardia ID by MALDI	76346-6
Reflex 1
Order Code	183406
Order Name	Nocardia ID by MALDI
Result Code	183406
Result Name	Nocardia ID by MALDI
UofM
Result LOINC	76346-6

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183407	Mycobacteria ID by MALDI	183407	Mycobacteria ID by MALDI	76346-6
Reflex 1
Order Code	183407
Order Name	Mycobacteria ID by MALDI
Result Code	183407
Result Name	Mycobacteria ID by MALDI
UofM
Result LOINC	76346-6

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183408	Yeast ID by MALDI	183408	Yeast ID by MALDI	76346-6
Reflex 1
Order Code	183408
Order Name	Yeast ID by MALDI
Result Code	183408
Result Name	Yeast ID by MALDI
UofM
Result LOINC	76346-6

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183428	Mold ID by MALDI	183428	Mold ID by MALDI	98404-7
Reflex 1
Order Code	183428
Order Name	Mold ID by MALDI
Result Code	183428
Result Name	Mold ID by MALDI
UofM
Result LOINC	98404-7

Reflex Table for Organism ID by MALDI
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	183327	Mold, ID by Sequencing	183328	Mold, ID by Sequencing	42804-5
Reflex 1
Order Code	183327
Order Name	Mold, ID by Sequencing
Result Code	183328
Result Name	Mold, ID by Sequencing
UofM
Result LOINC	42804-5

Managing Your Health

Diseases & Therapeutic Areas

Treatment Modalities & Methods

Scientific Lab Disciplines

Labs

Industries