Immunofixation (IFE) and Protein Electrophoresis, Random Urine

TEST: 122390
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CPT: 84156; 84166; 86335
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Synonyms

  • Bence Jones Protein Identification, Random Urine
  • Globulins, Random Urine
  • Myeloma Proteins, Random Urine
  • Urine Immunofixation for Myeloma Proteins

Test Includes

α1-Globulin, urine; α2-globulin, urine; β-globulin, urine; γ-globulin, urine; M-spike percentage; protein immunofixation, urine

Special Instructions

The request form should include patient's age and clinical diagnosis.

Expected Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Specimen Requirements

Specimen

Urine (random) (For 24-hour urine specimens, order test 003467.)

Volume

30-mL aliquot of random urine

Minimum Volume

11-mL aliquot of random urine

Container

12-mL plastic transport tube (Labcorp No. 23597) without preservative

Collection

Collect first morning urine; mix well.

Storage Instructions

Refrigerated (preferred) or room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x1

Causes for Rejection

Preservative added

Test Details

Use

Characterize the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ-globulin, γ-globulin fragments (light chains, Bence Jones proteins); of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström, and amyloidosis; work up monoclonal gammopathy (M-spike)

Methodology

Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
122390 IFE and PE, Random Urine 013664 Protein,Total,Urine mg/dL 2888-6
122390 IFE and PE, Random Urine 133751 Albumin, U % 13986-5
122390 IFE and PE, Random Urine 133769 Alpha-1-Globulin, U % 13984-0
122390 IFE and PE, Random Urine 133777 Alpha-2-Globulin, U % 13987-3
122390 IFE and PE, Random Urine 133785 Beta Globulin, U % 13994-9
122390 IFE and PE, Random Urine 133793 Gamma Globulin, U % 13989-9
122390 IFE and PE, Random Urine 133794 M-Spike, % % 42484-6
122390 IFE and PE, Random Urine 013482 Immunofixation Result, Urine 13440-3
122390 IFE and PE, Random Urine 149533 Note: N/A
122390 IFE and PE, Random Urine 003724 PDF 11502-2

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf