Immunofixation (IFE) and Protein Electrophoresis, 24-Hour Urine

TEST: 003467
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CPT: 84156; 84166; 86335
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Synonyms

  • Bence Jones Protein Identification, 24-Hour Urine
  • Globulins, 24-Hour Urine
  • IFE/Protein Electrophoresis, 24-Hour Urine
  • Myeloma Proteins, 24-Hour Urine

Test Includes

Urine immunofixation; qualitative identification of light chains and urine protein electrophoresis

Special Instructions

The request form should include patient's age and clinical diagnosis.

Expected Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

Specimen Requirements

Specimen

Urine (24-hour); use test 122390 if random urine is obtained.

Volume

30-mL aliquot

Minimum Volume

11-mL aliquot (Note: This volume does not allow for repeat testing.)

Container

12-mL plastic transport tube (Labcorp No. 23597) without preservative

Collection

Collect 24-hour urine; mix well.

Storage Instructions

Refrigerated (preferred) or room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x1

Causes for Rejection

Preservative added

Test Details

Use

Useful in characterizing the species of protein or proteins in cases of proteinuria. Techniques can identify albumin, γ globulin, γ globulin fragments (light chains, Bence Jones proteins). Of value in the study of monoclonal gammopathies, lymphoproliferative diseases, including lymphoma; special application to multiple myeloma, macroglobulinemia of Waldenström and amyloidosis. Monoclonal gammopathy (M spike) work-up.

Methodology

Urine immunofixation (IFE) on agarose gel; urine protein electrophoresis on agarose media; visual light-chain typing

Reference Interval

Normal pattern

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 013664 Protein,Total,Urine mg/dL 2888-6
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 013219 Prot,24hr calculated mg/24 hr 2889-4
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133751 Albumin, U % 13986-5
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133769 Alpha-1-Globulin, U % 13984-0
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133777 Alpha-2-Globulin, U % 13987-3
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133785 Beta Globulin, U % 13994-9
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133793 Gamma Globulin, U % 13989-9
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133794 M-Spike, % % 42484-6
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 133796 M-Spike, mg/24 hr mg/24 hr 42482-0
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 013482 Immunofixation Result, Urine 13440-3
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 149533 Note: N/A
003467 IFE+Protein Electro, 24-Hr Ur 95801-7 003724 PDF 11502-2

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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf