T-Cell Receptor γ-Chain Gene Rearrangements

TEST: 480708
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CPT: 81342
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Special Instructions

Please direct any questions regarding this test to customer service at 800-345-4363.

Expected Turnaround Time

5 - 7 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Specimen Requirements

Specimen

Whole blood, bone marrow, formalin-fixed, paraffin-embedded (FFPE) tissue block or slides, fresh or frozen tissue

Volume

3 to 5 mL whole blood, 1 to 2 mL bone marrow, five unstained slides at 10 µM or formalin-fixed, paraffin-embedded tissue block, tissue in RPMI or frozen

Minimum Volume

0.5 mL whole blood, 0.5 mL bone marrow, two unstained slides at 10 µM

Container

Lavender-top (EDTA) tube, yellow-top (ACD) tube, green-top (heparin) tube, tan-top (K2-EDTA) tube and pink-top (K2-EDTA) tube, FFPE tissue block or slides, tissue in RPMI or frozen

Collection

Fresh tissue should be placed in RPMI medium or shipped frozen on dry ice. Small samples (eg, punch biopsies, fine-needle aspirates) will also be accepted. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate specimens for each test requested.

Storage Instructions

Maintain whole blood/bone marrow or tissue in RPMI at 2°C to 8°C; FFPE specimens at room room temperature; frozen tissue at -80°C.

Causes for Rejection

Frozen whole blood/bone marrow; quantity not sufficient for analysis; tumor block containing no tumor tissue; broken or stained slides

Test Details

Use

This assay can be used to detect clonal T-cell receptor γ-chain gene rearrangements in blood, bone marrow, and tissue specimens. The presence of a monoclonal gene rearrangement usually, but not always, reflects the presence of a T-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive condition. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders.

Limitations

This PCR assay is able to detect a clonal DNA population at the sensitivity of 5% in a background of polyclonal DNA.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Methodology

Polymerase chain reaction (PCR)

References

Bruggemann M, White H, Gaulard P, et al. Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936. Leukemia. 2007 Feb;21(2):215-221.17170730
Langerak AW, Groenen PJ, Bruggemann M, et al. EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. Leukemia. 2012 Oct;26(10):2159-2171.22918122

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
480708 T-Cell Gene Rearrangement, PCR 480709 T-Cell PCR Interpretation 21753-9
480708 T-Cell Gene Rearrangement, PCR 480826 T Clonal Size N/A
480708 T-Cell Gene Rearrangement, PCR 481156 Block Number: N/A
480708 T-Cell Gene Rearrangement, PCR 489024 Director Review: 72486-4
480708 T-Cell Gene Rearrangement, PCR 480711 Methodology: 49549-9
480708 T-Cell Gene Rearrangement, PCR 481531 References: N/A

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