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T-Cell Receptor Gene Rearrangements Profile, γ and β

CPT 81340; 81342
Synonyms
  • TCRB Gene Clonality Assay
  • TCRG Gene Clonality Assay

Test Details

Methodology

Polymerase chain reaction (PCR)

Result Turnaround Time

5 - 7 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Use

This profile can be used to detect clonal T-cell receptor γ-chain (TCRG) and β-chain (TCRB) gene rearrangements in blood, bone marrow, and tissue specimens with a combined T-cell clonality detection rate of 94% to 99%. The presence of a monoclonal gene rearrangement usually, but not always, reflects the presence of a T-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive conditions. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders.

Special Instructions

Please direct any questions regarding this test to customer service at 800-345-4363.

Limitations

This PCR assay is able to detect a clonal DNA population at the sensitivity of 5% in a background of polyclonal DNA.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

References

Bruggemann M, White H, Gaulard P, et al. Powerful strategy for polymerase chain reaction-based clonality assessment in T-cell malignancies Report of the BIOMED-2 Concerted Action BHM4 CT98-3936. Leukemia. 2007 Feb;21(2):215-221.17170730
Langerak AW, Groenen PJ, Bruggemann M, et al. EuroClonality/BIOMED-2 guidelines for interpretation and reporting of Ig/TCR clonality testing in suspected lymphoproliferations. Leukemia. 2012 Oct;26(10):2159-2171.22918122

Specimen Requirements

Specimen

Whole blood, bone marrow, formalin-fixed, paraffin-embedded (FFPE) tissue block or slides, fresh or frozen tissue

Volume

3 to 5 mL whole blood, 1 mL bone marrow, five unstained slides at 10 μM or formalin-fixed, paraffin-embedded tissue block, tissue in RPMI or frozen

Minimum Volume

0.5 mL whole blood, 0.5 mL bone marrow, two unstained slides at 10 μM

Container

Lavender-top (EDTA) tube, yellow-top (ACD) tube, green-top (heparin) tube, tan-top (K2-EDTA) tube and pink-top (K2-EDTA) tube, FFPE tissue block or slides, tissue in RPMI or frozen

Collection Instructions

Fresh tissue should be placed in RPMI medium or shipped frozen on dry ice. Small samples (eg, punch biopsies, fine-needle aspirate) will also be accepted. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate specimens for each test requested.

Storage Instructions

Maintain blood, bone marrow or tissue in RPMI at 2°C to 8°C; FFPE specimens at room temperature; tissue at -80°C.

Causes for Rejection

Frozen whole blood/bone marrow; quantity not sufficient for analysis; tumor block containing no tumor tissue; broken or stained slides

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
481080 T Cell Panel (gamma, beta) 480709 T-Cell PCR Interpretation 21753-9
481080 T Cell Panel (gamma, beta) 480826 T Clonal Size N/A
481080 T Cell Panel (gamma, beta) 481156 Block Number: N/A
481080 T Cell Panel (gamma, beta) 489024 Director Review: 72486-4
481080 T Cell Panel (gamma, beta) 480711 Methodology: 49549-9
481080 T Cell Panel (gamma, beta) 481531 References: N/A
481080 T Cell Panel (gamma, beta) 480986 TCRB Gene Clonality Results: 21751-3
481080 T Cell Panel (gamma, beta) 480987 Clonal Size: N/A
481080 T Cell Panel (gamma, beta) 480989 Block Number: N/A
481080 T Cell Panel (gamma, beta) 480993 Director Review: 72486-4
481080 T Cell Panel (gamma, beta) 480984 Methodology: 49549-9
481080 T Cell Panel (gamma, beta) 480994 References: N/A
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480709
Result Code NameT-Cell PCR Interpretation
UofM
Result LOINC21753-9
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480826
Result Code NameT Clonal Size
UofM
Result LOINCN/A
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code481156
Result Code NameBlock Number:
UofM
Result LOINCN/A
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code489024
Result Code NameDirector Review:
UofM
Result LOINC72486-4
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480711
Result Code NameMethodology:
UofM
Result LOINC49549-9
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code481531
Result Code NameReferences:
UofM
Result LOINCN/A
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480986
Result Code NameTCRB Gene Clonality Results:
UofM
Result LOINC21751-3
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480987
Result Code NameClonal Size:
UofM
Result LOINCN/A
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480989
Result Code NameBlock Number:
UofM
Result LOINCN/A
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480993
Result Code NameDirector Review:
UofM
Result LOINC72486-4
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480984
Result Code NameMethodology:
UofM
Result LOINC49549-9
Order Code481080
Order Code NameT Cell Panel (gamma, beta)
Order Loinc
Result Code480994
Result Code NameReferences:
UofM
Result LOINCN/A