UrquhartBEBPA-EU19.pdf

BEBPA EU 2019 -- Because clients have enquired if Covance can perform cell-based 384-well assays, we initiated a proof of concept (POC) study to transfer the TNFa manual 96-well assay to 384-well format using Echo� Liquid Handler. 384-well assays allow increased number of samples or replicates tested/assay. 384-well assays can be implemented during assay development, allowing more variables to be tested/assay, resulting in quicker assay development and progression to QC environment. Due to 4-fold miniaturisation from 96-well format, 384-well assays would benefit from automation to provide consistency in cell and reagent dispense. The manual 96-well TNFa cytotoxicity assay is robust, precise and accurate; therefore, it is an excellent model for a transfer POC study to a small volume 384-well plate assay.