Hepatitis B Virus (HBV) DNA, Quantitative Real-time PCR With Reflex to HBV Genotype

TEST: 551722
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CPT: 87517. If reflex testing is performed, concomitant CPT codes/charges will apply.
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Synonyms

  • HBV Quantitation
  • HBV Viral Load

Expected Turnaround Time

7 - 9 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Specimen Requirements

Specimen

Plasma or serum, frozen

Volume

3 mL

Minimum Volume

1.5 mL

Container

Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or PPT. Do not use green-top (heparin) or yellow-top (ACD) tube

Collection

Centrifuge sample within six hours of collection. Transfer the serum/plasma to a screw-top polypropylene tube and freeze.

Storage Instructions

Freeze.

Causes for Rejection

Green-top (heparin) or yellow-top (ACD) tubes used for collection; serum/plasma not frozen; PPT not centrifuged

Test Details

Use

This test quantitates HBV DNA in serum or plasma with reflex to HBV genotype if ≥500 IU/mL is detected.

Limitations

The quantitative test has a range of 10 IU/mL to 1,000,000,000 IU/mL. The genotype assay is only triggered when the quantitative test result is ≥500 IU/mL.

Methodology

Quantitative test: cobas® HBV for use on the cobas® 6800/8800 system

Genotype test (reflex): Polymerase chain reaction (PCR) amplification and DNA sequencing

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551722 HBV Quant PCR Rfx to Genotype 42595-9 000000
551722 HBV Quant PCR Rfx to Genotype 551585 log10 HBV as IU/mL log10 IU/mL 48398-2
551722 HBV Quant PCR Rfx to Genotype 551651 HBV IU/mL IU/mL 42595-9
551722 HBV Quant PCR Rfx to Genotype 551724 Test Information: 19147-8
551722 HBV Quant PCR Rfx to Genotype 551726 HBV Genotype N/A

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf