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Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Graphical) With Reflex to HIV-1 GenoSure PRIme® or GenoSure Archive®

Test Number 550920
Test number copied
CPT 87536
Synonyms
  • HIV-1 Plasma Viremia
  • HiV-1 RNA Real-time PCR, Quantitative
  • Reflex to GenoSure Archive®
  • Reflex to GenoSure PRIme®
  • Viral Load

Test Details

Methodology

Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0 and polymerase chain reaction (PCR) amplification and DNA sequencing

Result Turnaround Time

4 - 6 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Use

Detect and measure HIV-1 in plasma with reflex to GenoSure PRIme® if viral load is greater than or equal to 500 copies/mL or reflex to GenoSure Archive® if viral load is less than 500 copies/mL. The GenoSure PRIme® and GenoSure Archive® assays detect mutations in the HIV viral genes that encode for HIV protease, reverse transcriptase and integrase. Mutations in these genes have been associated with resistance to antiretroviral drugs.

Special Instructions

If reflex test is performed, additional charges/CPT code(s) may apply.

Limitations

This procedure should only be used for patients with a documented HIV-1 infection.

Specimen Requirements

Specimen

Plasma and whole blood

Volume

5 mL plasma and 4 mL EDTA whole blood

Minimum Volume

3.5 mL plasma and 3 mL EDTA whole blood

Container

Lavender-top (EDTA) tube, screw-capped polypropylene frozen transport tube

Collection Instructions

Both frozen plasma and frozen whole blood are required for this profile. Draw three lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes.

Plasma: Centrifuge two tubes within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship specimen frozen.

Whole blood: Do not centrifuge. Freeze tube immediately after draw. Ship specimen frozen.

To avoid delays in turnaround time, when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Stability Requirements

Frozen: 6 weeks

Storage Instructions

Freeze.

Causes for Rejection

Insufficient volume; heparinized plasma; specimen not frozen

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550920 RNA PCR Graph Rfx PRIme/Arch 20447-9 550445 HIV-1 RNA by PCR copies/mL 20447-9
550920 RNA PCR Graph Rfx PRIme/Arch 20447-9 550446 log10 HIV-1 RNA log10copy/mL 29541-0
550920 RNA PCR Graph Rfx PRIme/Arch 20447-9 550703 Reflex to PRIme or Archive N/A
550920 RNA PCR Graph Rfx PRIme/Arch 20447-9 511958 PDF 80563-0
Order Code550920
Order Code NameRNA PCR Graph Rfx PRIme/Arch
Order Loinc20447-9
Result Code550445
Result Code NameHIV-1 RNA by PCR
UofMcopies/mL
Result LOINC20447-9
Order Code550920
Order Code NameRNA PCR Graph Rfx PRIme/Arch
Order Loinc20447-9
Result Code550446
Result Code Namelog10 HIV-1 RNA
UofMlog10copy/mL
Result LOINC29541-0
Order Code550920
Order Code NameRNA PCR Graph Rfx PRIme/Arch
Order Loinc20447-9
Result Code550703
Result Code NameReflex to PRIme or Archive
UofM
Result LOINCN/A
Order Code550920
Order Code NameRNA PCR Graph Rfx PRIme/Arch
Order Loinc20447-9
Result Code511958
Result Code NamePDF
UofM
Result LOINC80563-0
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 552039 GenoSure PRIme(R) 552019 PDF 51969-4
Reflex 1
Order Code552039
Order NameGenoSure PRIme(R)
Result Code552019
Result NamePDF
UofM
Result LOINC51969-4
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 552039 GenoSure PRIme(R) 552021 HIV GenoSure PRIme(R) 80689-3
Reflex 1
Order Code552039
Order NameGenoSure PRIme(R)
Result Code552021
Result NameHIV GenoSure PRIme(R)
UofM
Result LOINC80689-3
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 552039 GenoSure PRIme(R) 552022 HIV GenoSure PRIme(R) Interp 62364-5
Reflex 1
Order Code552039
Order NameGenoSure PRIme(R)
Result Code552022
Result NameHIV GenoSure PRIme(R) Interp
UofM
Result LOINC62364-5
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551774 PDF 80695-0
Reflex 1
Order Code551798
Order NameGenoSure Archive(R)
Result Code551774
Result NamePDF
UofM
Result LOINC80695-0
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551777 HIV GenoSure Archive 93047-9
Reflex 1
Order Code551798
Order NameGenoSure Archive(R)
Result Code551777
Result NameHIV GenoSure Archive
UofM
Result LOINC93047-9
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551778 HIV GenoSure Archive 80693-5
Reflex 1
Order Code551798
Order NameGenoSure Archive(R)
Result Code551778
Result NameHIV GenoSure Archive
UofM
Result LOINC80693-5
Reflex Table for Reflex to PRIme or Archive
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551798 GenoSure Archive(R) 551779 HIV GenoSure Archive Interp 80694-3
Reflex 1
Order Code551798
Order NameGenoSure Archive(R)
Result Code551779
Result NameHIV GenoSure Archive Interp
UofM
Result LOINC80694-3