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Human Immunodeficiency Virus 1 (HIV-1), Quantitative, RNA (Abbott® RealTime)

CPT 87536
Synonyms
  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time (RT) PCR, Quantitative
  • Viral Load, Abbott, Real-time

Test Details

Methodology

Abbott Alinity m HIV-1 assay

Result Turnaround Time

3 - 7 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Use

Detect and quantitate HIV-1 in plasma

Limitations

Assay range: 20−10,000,000 copies/mL

Specimen Requirements

Specimen

Plasma, frozen

Volume

2 mL

Minimum Volume

1.1 mL (Note: This volume does not allow for repeat testing.)

Container

Ship in a screw-cap polypropylene frozen transport tube or centrifuged PPT™ tubes.

Collection Instructions

Collect specimen in lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Centrifuge specimen. If drawn in EDTA or ACD, the plasma must be transferred to a screw-cap polypropylene transport tube and frozen. If refrigerated, whole blood specimen may be held for 48 hours prior to centrifugation. If room temperature, whole blood specimen may be held for 24 hours prior to centrifugation. Plasma specimens may be stored at -20°C for 60 days. If longer storage is required, plasma specimens may be stored at -70°C or lower for 6 months. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Stability Requirements

Temperature

Period

Room temperature

24 hours

Refrigerated

72 hours

Frozen

-20°C = 60 days

-70°C = 6 months

Freeze/thaw cycles

Stable x0

Storage Instructions

Frozen (preferred)

Causes for Rejection

Hemolysis; plasma other than EDTA or ACD-A; quantity not sufficient for analysis

References

Huang S, Salituro J, Tang N, et al. Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucleic Acids Res. 2007; 35(16):e101.17693434
Tang N, Huang S, Salituro J, et al. A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples. J Virol Methods. 2007; 146(1-2):236-245. 17707519

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550880 RealTime Abbott 75622-1 550881 HIV-1 RNA by PCR copies/mL 20447-9
550880 RealTime Abbott 75622-1 550882 log10 HIV-1 RNA log10copy/mL 29541-0
Order Code550880
Order Code NameRealTime Abbott
Order Loinc75622-1
Result Code550881
Result Code NameHIV-1 RNA by PCR
UofMcopies/mL
Result LOINC20447-9
Order Code550880
Order Code NameRealTime Abbott
Order Loinc75622-1
Result Code550882
Result Code Namelog10 HIV-1 RNA
UofMlog10copy/mL
Result LOINC29541-0