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Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 GenoSure PRIme® or GenoSure Archive®
CPT:
87536
Synonyms
- HIV-1 Plasma Viremia
- HiV-1 RNA Real-time PCR, Quantitative
- Reflex to GenoSure Archive®
- Reflex to GenoSure PRIme®
- Viral Load
Special Instructions
If reflex test is performed, additional charges/CPT code(s) may apply.
Expected Turnaround Time
4 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Plasma and whole blood
Volume
5 mL plasma and 4 mL EDTA whole blood
Minimum Volume
3.5 mL plasma and 3 mL EDTA whole blood
Container
Lavender-top (EDTA) tube, screw-capped polypropylene frozen transport tube
Collection
Both frozen plasma and frozen whole blood are required for this profile. Draw three lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes.
Plasma: Centrifuge two tubes within six hours of collection. Remove plasma, transfer plasma to screw-cap polypropylene transport tubes (not "pop-top" or "snap-cap"), and freeze. Ship specimen frozen.
Whole blood: Do not centrifuge. Freeze tube immediately after draw. Ship specimen frozen.
To avoid delays in turnaround time, when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Stability Requirements
Frozen: 6 weeks
Causes for Rejection
Insufficient volume; heparinized plasma; specimen not frozen
Test Details
Use
Detect and measure HIV-1 in plasma with reflex to GenoSure PRIme® if viral load is greater than or equal to 500 copies/mL or reflex to GenoSure Archive® if viral load is less than 500 copies/mL. The GenoSure PRIme® and GenoSure Archive® assays detect mutations in the HIV viral genes that encode for HIV protease, reverse transcriptase and integrase. Mutations in these genes have been associated with resistance to antiretroviral drugs.
Limitations
This procedure should only be used for patients with a documented HIV-1 infection.
Methodology
Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0 and polymerase chain reaction (PCR) amplification and DNA sequencing
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550675 | RNA PCR NonGraph Rfx PRIme/Arc | Pending | 550445 | HIV-1 RNA by PCR | copies/mL | 20447-9 |
| 550675 | RNA PCR NonGraph Rfx PRIme/Arc | Pending | 550446 | log10 HIV-1 RNA | log10copy/mL | 29541-0 |
| 550675 | RNA PCR NonGraph Rfx PRIme/Arc | Pending | 550703 | Reflex to PRIme or Archive | N/A |
| Reflex Table for Reflex to PRIme or Archive | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 552039 | GenoSure PRIme(R) | 552019 | 51969-4 | ||
| Reflex Table for Reflex to PRIme or Archive | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 552039 | GenoSure PRIme(R) | 552021 | HIV GenoSure PRIme(R) | 80689-3 | |
| Reflex Table for Reflex to PRIme or Archive | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 552039 | GenoSure PRIme(R) | 552022 | HIV GenoSure PRIme(R) Interp | 62364-5 | |
| Reflex Table for Reflex to PRIme or Archive | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551798 | GenoSure Archive(R) | 551774 | 80695-0 | ||
| Reflex Table for Reflex to PRIme or Archive | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551798 | GenoSure Archive(R) | 551777 | HIV GenoSure Archive | 93047-9 | |
| Reflex 2 | 551781 | HIV GSArchive | 551781 | HIV GSArchive | N/A | |
| Reflex Table for Reflex to PRIme or Archive | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551798 | GenoSure Archive(R) | 551778 | HIV GenoSure Archive | 80693-5 | |
| Reflex 2 | 551782 | HIV GSArchive Interp | 551782 | HIV GSArchive Interp | N/A | |
| Reflex Table for Reflex to PRIme or Archive | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 551798 | GenoSure Archive(R) | 551779 | HIV GenoSure Archive Interp | 80694-3 | |
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CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf