Test Details
Methodology
Polymerase chain reaction (PCR) amplification and solid-phase electrochemical detection
Result Turnaround Time
12 - 18 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Genotyping of the six major HCV types and their most common subtypes. Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5A inhibitors. Detects resistance-associated variants at positions 28, 30, 31, and 93 in genotype 1a HCV.
Limitations
The HCV genotype determination may not be successful when the HCV viral load is <1000 IU/mL serum/plasma. The HCV NS5A drug resistance assay will only be performed when the HCV genotype is 1a.
Specimen Requirements
Specimen
Plasma (preferred) or serum, frozen
Volume
3 mL
Minimum Volume
2 mL
Container
Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.
Collection Instructions
Collect specimen in two PPT™ tubes, gel-barrier tubes, yellow-top (ACD) tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; green-top (heparin) tube; specimen not frozen on date of collection; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; specimen shipped at room temperature
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550615 | HCV Genotype Reflex NS5A | 32286-7 | 550617 | Hepatitis C Genotype | 32286-7 | |
| 550615 | HCV Genotype Reflex NS5A | 32286-7 | 550618 | Please note: | 77202-0 | |
| Order Code | 550615 | |||||
| Order Code Name | HCV Genotype Reflex NS5A | |||||
| Order Loinc | 32286-7 | |||||
| Result Code | 550617 | |||||
| Result Code Name | Hepatitis C Genotype | |||||
| UofM | ||||||
| Result LOINC | 32286-7 | |||||
| Order Code | 550615 | |||||
| Order Code Name | HCV Genotype Reflex NS5A | |||||
| Order Loinc | 32286-7 | |||||
| Result Code | 550618 | |||||
| Result Code Name | Please note: | |||||
| UofM | ||||||
| Result LOINC | 77202-0 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550326 | HCV NS5A Drug Resistance Assay | 550316 | HCV NS5A Resistance Assay PDF | 72862-6 | |
| Reflex 1 | ||||||
| Order Code | 550326 | |||||
| Order Name | HCV NS5A Drug Resistance Assay | |||||
| Result Code | 550316 | |||||
| Result Name | HCV NS5A Resistance Assay PDF | |||||
| UofM | ||||||
| Result LOINC | 72862-6 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550326 | HCV NS5A Drug Resistance Assay | 550317 | HCV NS5A Drug Resistance Assay | 73655-3 | |
| Reflex 1 | ||||||
| Order Code | 550326 | |||||
| Order Name | HCV NS5A Drug Resistance Assay | |||||
| Result Code | 550317 | |||||
| Result Name | HCV NS5A Drug Resistance Assay | |||||
| UofM | ||||||
| Result LOINC | 73655-3 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550326 | HCV NS5A Drug Resistance Assay | 550318 | HCV NS5A Resist Assay Interp | 49549-9 | |
| Reflex 1 | ||||||
| Order Code | 550326 | |||||
| Order Name | HCV NS5A Drug Resistance Assay | |||||
| Result Code | 550318 | |||||
| Result Name | HCV NS5A Resist Assay Interp | |||||
| UofM | ||||||
| Result LOINC | 49549-9 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550621 | Reflex Test Information | 550621 | Reflex Test Information | N/A | |
| Reflex 1 | ||||||
| Order Code | 550621 | |||||
| Order Name | Reflex Test Information | |||||
| Result Code | 550621 | |||||
| Result Name | Reflex Test Information | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||