Test Details
Methodology
Cobas® AmpliPrep/Cobas® Taqman® HCV Test v 2.0. See Hepatitis C Virus (HCV) NS5B Drug Resistance Assay [550505] for more information.
Result Turnaround Time
11 - 17 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Reflex to HCV NS5B drug resistance assay if viral load is ≥1000 IU/mL
Use
Quantification of HCV with reflex to HCV NS5B drug resistance assay (at additional charge) if ≥1000 IU/mL.
Special Instructions
Please indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit the patient's Social Security number (or unique identifier) to order serial monitoring.
Limitations
The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.
Specimen Requirements
Specimen
Plasma (preferred) or serum, frozen
Volume
4 mL
Minimum Volume
2 mL
Container
Ship in plasma preparation tube (PPT™) or screw-capped polyproplylene frozen transport tubes.
Collection Instructions
Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Storage Instructions
Freeze.
Causes for Rejection
Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550554 | HCV RNA PCR Qn(Graph) Rfx NS5B | 50023-1 | 550073 | Hepatitis C Quantitation | IU/mL | 11011-4 |
| 550554 | HCV RNA PCR Qn(Graph) Rfx NS5B | 50023-1 | 550074 | HCV log10 | log10 IU/mL | 38180-6 |
| 550554 | HCV RNA PCR Qn(Graph) Rfx NS5B | 50023-1 | 550308 | Test Information: | N/A | |
| 550554 | HCV RNA PCR Qn(Graph) Rfx NS5B | 50023-1 | 550528 | HCV NS5B Drug Resistance Assay | N/A | |
| 550554 | HCV RNA PCR Qn(Graph) Rfx NS5B | 50023-1 | 511958 | 80563-0 | ||
| Order Code | 550554 | |||||
| Order Code Name | HCV RNA PCR Qn(Graph) Rfx NS5B | |||||
| Order Loinc | 50023-1 | |||||
| Result Code | 550073 | |||||
| Result Code Name | Hepatitis C Quantitation | |||||
| UofM | IU/mL | |||||
| Result LOINC | 11011-4 | |||||
| Order Code | 550554 | |||||
| Order Code Name | HCV RNA PCR Qn(Graph) Rfx NS5B | |||||
| Order Loinc | 50023-1 | |||||
| Result Code | 550074 | |||||
| Result Code Name | HCV log10 | |||||
| UofM | log10 IU/mL | |||||
| Result LOINC | 38180-6 | |||||
| Order Code | 550554 | |||||
| Order Code Name | HCV RNA PCR Qn(Graph) Rfx NS5B | |||||
| Order Loinc | 50023-1 | |||||
| Result Code | 550308 | |||||
| Result Code Name | Test Information: | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 550554 | |||||
| Order Code Name | HCV RNA PCR Qn(Graph) Rfx NS5B | |||||
| Order Loinc | 50023-1 | |||||
| Result Code | 550528 | |||||
| Result Code Name | HCV NS5B Drug Resistance Assay | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 550554 | |||||
| Order Code Name | HCV RNA PCR Qn(Graph) Rfx NS5B | |||||
| Order Loinc | 50023-1 | |||||
| Result Code | 511958 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 80563-0 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550075 | HCV RNA (International Units) | 550078 | HCV RNA (International Units) | IU/mL | 11011-4 |
| Reflex 1 | ||||||
| Order Code | 550075 | |||||
| Order Name | HCV RNA (International Units) | |||||
| Result Code | 550078 | |||||
| Result Name | HCV RNA (International Units) | |||||
| UofM | IU/mL | |||||
| Result LOINC | 11011-4 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550075 | HCV RNA (International Units) | 550081 | HCV log10 | log10 IU/mL | 38180-6 |
| Reflex 1 | ||||||
| Order Code | 550075 | |||||
| Order Name | HCV RNA (International Units) | |||||
| Result Code | 550081 | |||||
| Result Name | HCV log10 | |||||
| UofM | log10 IU/mL | |||||
| Result LOINC | 38180-6 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550527 | HCV NS5B Drug Resistance Assay | 550506 | HCV NS5B Resistance Assay PDF | 72862-6 | |
| Reflex 1 | ||||||
| Order Code | 550527 | |||||
| Order Name | HCV NS5B Drug Resistance Assay | |||||
| Result Code | 550506 | |||||
| Result Name | HCV NS5B Resistance Assay PDF | |||||
| UofM | ||||||
| Result LOINC | 72862-6 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550527 | HCV NS5B Drug Resistance Assay | 550507 | HCV NS5B Drug Resistance Assay | 73655-3 | |
| Reflex 1 | ||||||
| Order Code | 550527 | |||||
| Order Name | HCV NS5B Drug Resistance Assay | |||||
| Result Code | 550507 | |||||
| Result Name | HCV NS5B Drug Resistance Assay | |||||
| UofM | ||||||
| Result LOINC | 73655-3 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550527 | HCV NS5B Drug Resistance Assay | 550516 | HCV NS5B Resist Assay Interp | 49549-9 | |
| Reflex 1 | ||||||
| Order Code | 550527 | |||||
| Order Name | HCV NS5B Drug Resistance Assay | |||||
| Result Code | 550516 | |||||
| Result Name | HCV NS5B Resist Assay Interp | |||||
| UofM | ||||||
| Result LOINC | 49549-9 | |||||