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Hepatitis C Virus (HCV), Quantitative, RNA PCR (Graphical) With Reflex to Hepatitis C Virus (HCV) NS5A Drug Resistance Assay

Test Number 550333
Test number copied
CPT 87522. If reflex testing is performed, concomitant CPT codes/charges will apply.
Synonyms
  • HCV Real-time PCR With Reflex to HCV NS5A Drug Resistance Assay
  • HCV RNA Quantitation With Reflex to HCV NS5A Drug Resistance Assay

Test Details

Methodology

cobas® HCV (Roche Molecular Systems). See Hepatitis C Virus (HCV) NS5A Drug Resistance Assay [550325] for more information.

Result Turnaround Time

11 - 17 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Test Includes

Reflex to HCV NS5A drug resistance assay if viral load is ≥500 IU/mL.

Use

Quantification of HCV with reflex to HCV NS5A drug resistance assay (at additional charge) if ≥500 IU/mL.

Special Instructions

Please indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit the patient's Social Security number (or unique identifier) to order serial monitoring.

Limitations

The HCV real-time PCR assay has a quantitative range of 15 IU/mL to 100,000,000 IU/mL. This assay requires an HCV genotype of either 1a or 1b.

Specimen Requirements

Specimen

Plasma (preferred) or serum, frozen

Volume

4 mL

Minimum Volume

2 mL

Container

Ship in plasma preparation tubes (PPT™) or screw-capped polypropylene frozen transport tubes.

Collection Instructions

Collect specimen in two PPT™ tubes, gel-barrier tubes, or lavender-top (EDTA) tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection, remove serum/plasma, transfer specimen to polypropylene screw-capped tubes, and freeze. Specimens drawn into PPT™ tubes need to be centrifuged within six hours and frozen. Ship frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze.

Causes for Rejection

Hemolysis; heparinized plasma; nonfrozen specimen; PPT™ not centrifuged; specimen received in "pop-top" or "snap-cap" tube; whole blood

References

Yao JD, Young S, Heilek GM, et al. Diagnosis and monitoring of HCV infection using the cobas® HCV test for use on the cobas® 6800/8800 systems. J Clin Virol. 2018 May;102:63-69.29518694

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550333 HCV RNA PCR Qn(Graph) Rfx NS5A 11011-4 550073 Hepatitis C Quantitation IU/mL 11011-4
550333 HCV RNA PCR Qn(Graph) Rfx NS5A 11011-4 550074 HCV log10 log10 IU/mL 38180-6
550333 HCV RNA PCR Qn(Graph) Rfx NS5A 11011-4 550308 Test Information: N/A
550333 HCV RNA PCR Qn(Graph) Rfx NS5A 11011-4 550332 HCV NS5A Drug Resistance Assay N/A
550333 HCV RNA PCR Qn(Graph) Rfx NS5A 11011-4 511958 PDF 80563-0
Order Code550333
Order Code NameHCV RNA PCR Qn(Graph) Rfx NS5A
Order Loinc11011-4
Result Code550073
Result Code NameHepatitis C Quantitation
UofMIU/mL
Result LOINC11011-4
Order Code550333
Order Code NameHCV RNA PCR Qn(Graph) Rfx NS5A
Order Loinc11011-4
Result Code550074
Result Code NameHCV log10
UofMlog10 IU/mL
Result LOINC38180-6
Order Code550333
Order Code NameHCV RNA PCR Qn(Graph) Rfx NS5A
Order Loinc11011-4
Result Code550308
Result Code NameTest Information:
UofM
Result LOINCN/A
Order Code550333
Order Code NameHCV RNA PCR Qn(Graph) Rfx NS5A
Order Loinc11011-4
Result Code550332
Result Code NameHCV NS5A Drug Resistance Assay
UofM
Result LOINCN/A
Order Code550333
Order Code NameHCV RNA PCR Qn(Graph) Rfx NS5A
Order Loinc11011-4
Result Code511958
Result Code NamePDF
UofM
Result LOINC80563-0
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550075 HCV RNA (International Units) 550078 HCV RNA (International Units) IU/mL 11011-4
Reflex 1
Order Code550075
Order NameHCV RNA (International Units)
Result Code550078
Result NameHCV RNA (International Units)
UofMIU/mL
Result LOINC11011-4
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550075 HCV RNA (International Units) 550081 HCV log10 log10 IU/mL 38180-6
Reflex 1
Order Code550075
Order NameHCV RNA (International Units)
Result Code550081
Result NameHCV log10
UofMlog10 IU/mL
Result LOINC38180-6
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550316 HCV NS5A Resistance Assay PDF 72862-6
Reflex 1
Order Code550326
Order NameHCV NS5A Drug Resistance Assay
Result Code550316
Result NameHCV NS5A Resistance Assay PDF
UofM
Result LOINC72862-6
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550317 HCV NS5A Drug Resistance Assay 73655-3
Reflex 1
Order Code550326
Order NameHCV NS5A Drug Resistance Assay
Result Code550317
Result NameHCV NS5A Drug Resistance Assay
UofM
Result LOINC73655-3
Reflex Table for HCV NS5A Drug Resistance Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550326 HCV NS5A Drug Resistance Assay 550318 HCV NS5A Resist Assay Interp 49549-9
Reflex 1
Order Code550326
Order NameHCV NS5A Drug Resistance Assay
Result Code550318
Result NameHCV NS5A Resist Assay Interp
UofM
Result LOINC49549-9