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Anti-ENA6 Plus DFS70Ab Profile

CPT 83516; 86235(x6)
Synonyms
  • Anti-Extractable Nuclear Antigens (ENA) 6 Plus Anti-Dense Fine Speckled 70 Antibodies Profile

Test Details

Methodology

See individual test components.

Result Turnaround Time

14 - 21 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Test Includes

Anti-DFS70; Anti-Sm; Anti-U1 RNP; Anti-Ro (SS-A); Anti-La (SS-B); Anti-Scl-70; Anti-Jo-1

Use

Anti-ENA6 Plus DFS70 is designed to facilitate the proper triage of patients with positive Anti-Nuclear Antibodies (ANA) by identifying those most likely to benefit from a rheumatology specialist. It detects the novel Anti-DFS70 along with 6 other clinically useful autoantibodies to ENA, which may help identify systemic lupus erythematous (SLE), mixed connective tissue disease (MCTD), Sjogren's, systemic sclerosis, and idiopathic inflammatory myopathy (IIM). Positive Anti-DFS70 antibodies in isolation may help identify individuals who do not have an AARD (ANA-associated rheumatic disease), especially in the absence of significant clinical findings.

Anti-DFS70 Abs confer a likelihood ratio for the absence of systemic autoimmune rheumatic disease of 10.9 and are detectable in 2% to 22% of healthy individuals and in less than 1% of patients with AARD.

Limitations

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

References

Conrad K, Röber N, Andrade LE, Mahler M. The Clinical Relevance of Anti-DFS70 Autoantibodies. Clin Rev Allergy Immunol. 2017 Apr;52(2):202-216.27350273
Fitch-Rogalsky C, Steber W, Mahler M, et al. Clinical and Serological Features of Patients Referred through a Rheumatology Triage System because of Positive Antinuclear Antibodies. PLoS One. 2014 Apr 4;9(4):e93812.24705829

Specimen Requirements

Specimen

Serum

Volume

3 mL

Minimum Volume

1.5 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection Instructions

Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.

Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

14 days

Frozen

60 days

Freeze/thaw cycles

Stable x1

Storage Instructions

Refrigerate.

Causes for Rejection

Grossly hemolyzed; bacterial contamination; lipemic, icteric, non-serum specimen types

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
520301 Anti-ENA6 Plus DFS70Ab Profile 520316 Anti-DFS70 Ab Units 95129-3
520301 Anti-ENA6 Plus DFS70Ab Profile 520216 Anti-Sm Ab (RDL) Units 11090-8
520301 Anti-ENA6 Plus DFS70Ab Profile 520217 Anti-U1 RNP Ab (RDL) Units 57662-9
520301 Anti-ENA6 Plus DFS70Ab Profile 520218 Anti-Ro (SS-A) Ab (RDL) Units 33569-5
520301 Anti-ENA6 Plus DFS70Ab Profile 520225 Anti-La (SS-B) Ab (RDL) Units 45142-7
520301 Anti-ENA6 Plus DFS70Ab Profile 520221 Anti-Scl-70 Ab (RDL) Units 26975-3
520301 Anti-ENA6 Plus DFS70Ab Profile 520259 Anti-Jo-1 Ab (RDL) Units 35333-4
Order Code520301
Order Code NameAnti-ENA6 Plus DFS70Ab Profile
Order Loinc
Result Code520316
Result Code NameAnti-DFS70 Ab
UofMUnits
Result LOINC95129-3
Order Code520301
Order Code NameAnti-ENA6 Plus DFS70Ab Profile
Order Loinc
Result Code520216
Result Code NameAnti-Sm Ab (RDL)
UofMUnits
Result LOINC11090-8
Order Code520301
Order Code NameAnti-ENA6 Plus DFS70Ab Profile
Order Loinc
Result Code520217
Result Code NameAnti-U1 RNP Ab (RDL)
UofMUnits
Result LOINC57662-9
Order Code520301
Order Code NameAnti-ENA6 Plus DFS70Ab Profile
Order Loinc
Result Code520218
Result Code NameAnti-Ro (SS-A) Ab (RDL)
UofMUnits
Result LOINC33569-5
Order Code520301
Order Code NameAnti-ENA6 Plus DFS70Ab Profile
Order Loinc
Result Code520225
Result Code NameAnti-La (SS-B) Ab (RDL)
UofMUnits
Result LOINC45142-7
Order Code520301
Order Code NameAnti-ENA6 Plus DFS70Ab Profile
Order Loinc
Result Code520221
Result Code NameAnti-Scl-70 Ab (RDL)
UofMUnits
Result LOINC26975-3
Order Code520301
Order Code NameAnti-ENA6 Plus DFS70Ab Profile
Order Loinc
Result Code520259
Result Code NameAnti-Jo-1 Ab (RDL)
UofMUnits
Result LOINC35333-4
Reflex Table for Anti-Scl-70 Ab (RDL)
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 520341 Repeat Anti-Scl-70 520341 Repeat Anti-Scl-70 5348-8
Reflex 1
Order Code520341
Order NameRepeat Anti-Scl-70
Result Code520341
Result NameRepeat Anti-Scl-70
UofM
Result LOINC5348-8