Test Details
Methodology
See individual test components.
Result Turnaround Time
10 - 12 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
This profile is composed of Anti-Nuclear Ab (ANA) by IFA, Anti-dsDNA Ab by Farr, Anti-Glomerular Basement Membrane Ab (GBM), Anti-Neutrophil Cytoplasmic Ab (ANCA), Anti-Myeloperoxidase Ab (MPO), Anti-Proteinase 3 Ab (PR-3), C3 Complement, C4 Complement.
ANA performed if ANCA positive to rule out false positive ANCA due to presence of ANA.
Use
ANA are commonly present in a variety of autoimmune diseases. ANA frequency also increases with age in apparently healthy people. ANA patterns on HEp-2 slides provide general clues about particles (chromatin, nucleosomes, and spliceosomes).
The Farr assay is the most specific method for detecting dsDNA autoantibodies. Significant elevations in dsDNA autoantibody concentrations confirm the diagnosis of systemic lupus erythematosus (SLE). Serial studies of elevated values of dsDNA auto antibodies are useful for predicting activity of SLE.
Anti-MPO autoantibodies aid in assessment of certain autoimmune vasculitides such as microscopic polyarteritis, and crescentic glomerulonephritis.
Elevated levels of Anti-PR-3 antibodies are classically observed in patients with Wegener granulomatosis (WG), particularly with active disease, and less frequently in other forms of necrotizing vasculitis.
Anti-GBM autoantibodies are recognized as being important in the pathogenesis of the rapidly progressive glomerulonephritis of Goodpasture's syndrome. Measurements of C3 and C4 are used to detect individuals with inborn deficiency of this factor or those with immunologic disease in whom complement is consumed at an increased rate. These include lupus erythematosus, chronic active hepatitis, certain chronic infections, post-streptococcal and membranoproliferative glomerulonephritis, and others.
Specimen Requirements
Specimen
Serum
Volume
4 mL
Minimum Volume
1.5 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Stability Requirements
Temperature | Period |
---|---|
Room temperature | 2 days |
Refrigerated | 8 days |
Frozen | 8 days |
Storage Instructions
Refrigerate or freeze.
LOINC® Map
Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
---|---|---|---|---|---|---|
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520073 | Anti-Nuclear Ab by IFA (RDL) | 42254-3 | |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520150 | ANCA by IFA (RDL) | 29641-8 | |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520211 | Anti-MPO Ab (RDL) | Units | 46266-3 |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520215 | Anti-PR-3 Ab (RDL) | Units | 46267-1 |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520249 | Anti-GBM Ab (RDL) | Units | 31406-2 |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520060 | Anti-dsDNA Ab by Farr(RDL) | IU/mL | 47299-3 |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520169 | C3 Complement (RDL) | mg/dL | 4485-9 |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520167 | C4 Complement (RDL) | mg/dL | 4498-2 |
520194 | Glomerulonephritis (GNP) Prof | 97564-9 | 520260 | Interpretation: | N/A | |
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520073 | |||||
Result Code Name | Anti-Nuclear Ab by IFA (RDL) | |||||
UofM | ||||||
Result LOINC | 42254-3 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520150 | |||||
Result Code Name | ANCA by IFA (RDL) | |||||
UofM | ||||||
Result LOINC | 29641-8 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520211 | |||||
Result Code Name | Anti-MPO Ab (RDL) | |||||
UofM | Units | |||||
Result LOINC | 46266-3 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520215 | |||||
Result Code Name | Anti-PR-3 Ab (RDL) | |||||
UofM | Units | |||||
Result LOINC | 46267-1 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520249 | |||||
Result Code Name | Anti-GBM Ab (RDL) | |||||
UofM | Units | |||||
Result LOINC | 31406-2 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520060 | |||||
Result Code Name | Anti-dsDNA Ab by Farr(RDL) | |||||
UofM | IU/mL | |||||
Result LOINC | 47299-3 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520169 | |||||
Result Code Name | C3 Complement (RDL) | |||||
UofM | mg/dL | |||||
Result LOINC | 4485-9 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520167 | |||||
Result Code Name | C4 Complement (RDL) | |||||
UofM | mg/dL | |||||
Result LOINC | 4498-2 | |||||
Order Code | 520194 | |||||
Order Code Name | Glomerulonephritis (GNP) Prof | |||||
Order Loinc | 97564-9 | |||||
Result Code | 520260 | |||||
Result Code Name | Interpretation: | |||||
UofM | ||||||
Result LOINC | N/A |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520074 | Homogeneous Pattern | 20398-4 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520074 | |||||
Result Name | Homogeneous Pattern | |||||
UofM | ||||||
Result LOINC | 20398-4 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520076 | Nucleolar Pattern | 20399-2 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520076 | |||||
Result Name | Nucleolar Pattern | |||||
UofM | ||||||
Result LOINC | 20399-2 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520233 | Speckled Pattern | 20401-6 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520233 | |||||
Result Name | Speckled Pattern | |||||
UofM | ||||||
Result LOINC | 20401-6 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520234 | Centromere Pattern | 53993-2 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520234 | |||||
Result Name | Centromere Pattern | |||||
UofM | ||||||
Result LOINC | 53993-2 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520235 | Spindle Apparatus Pattern | 53003-0 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520235 | |||||
Result Name | Spindle Apparatus Pattern | |||||
UofM | ||||||
Result LOINC | 53003-0 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520236 | Nuclear Membrane Pattern | 54006-2 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520236 | |||||
Result Name | Nuclear Membrane Pattern | |||||
UofM | ||||||
Result LOINC | 54006-2 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520237 | Midbody Pattern | 54155-7 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520237 | |||||
Result Name | Midbody Pattern | |||||
UofM | ||||||
Result LOINC | 54155-7 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520238 | Nuclear Dot Pattern | 53998-1 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520238 | |||||
Result Name | Nuclear Dot Pattern | |||||
UofM | ||||||
Result LOINC | 53998-1 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520239 | PCNA Pattern | 53006-3 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520239 | |||||
Result Name | PCNA Pattern | |||||
UofM | ||||||
Result LOINC | 53006-3 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520240 | Centriole Pattern | 53993-2 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520240 | |||||
Result Name | Centriole Pattern | |||||
UofM | ||||||
Result LOINC | 53993-2 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520077 | ANA Titer and Pattern | 520241 | Note: | 77202-0 | |
Reflex 1 | ||||||
Order Code | 520077 | |||||
Order Name | ANA Titer and Pattern | |||||
Result Code | 520241 | |||||
Result Name | Note: | |||||
UofM | ||||||
Result LOINC | 77202-0 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520257 | Positive ANCA Reflex | 520187 | C-ANCA (IFA-ETOH) | 14277-8 | |
Reflex 1 | ||||||
Order Code | 520257 | |||||
Order Name | Positive ANCA Reflex | |||||
Result Code | 520187 | |||||
Result Name | C-ANCA (IFA-ETOH) | |||||
UofM | ||||||
Result LOINC | 14277-8 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520257 | Positive ANCA Reflex | 520189 | P-ANCA (IFA-ETOH) | 14278-6 | |
Reflex 1 | ||||||
Order Code | 520257 | |||||
Order Name | Positive ANCA Reflex | |||||
Result Code | 520189 | |||||
Result Name | P-ANCA (IFA-ETOH) | |||||
UofM | ||||||
Result LOINC | 14278-6 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 520257 | Positive ANCA Reflex | 520191 | P-ANCA (IFA-Formalin) | 17357-5 | |
Reflex 1 | ||||||
Order Code | 520257 | |||||
Order Name | Positive ANCA Reflex | |||||
Result Code | 520191 | |||||
Result Name | P-ANCA (IFA-Formalin) | |||||
UofM | ||||||
Result LOINC | 17357-5 |