Rheumatoid Factor Isotypes, IgG, IgA, and IgM by EIA (RDL)

CPT: 86431(x3)
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Test Includes

The tests in this profile are not Rheumatoid Factor by Turbidimetry.


Expected Turnaround Time

14 - 21 days


Related Documents

For more information, please view the literature below.

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Specimen Requirements


Specimen

Serum


Volume

4 mL


Minimum Volume

3 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Collection

Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.


Storage Instructions

Refrigerate.


Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

14 days

Frozen

60 days

Freeze/thaw cycles

Stable x1


Causes for Rejection

Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types


Test Details


Use

EIA methods have the added advantage of being able to simultaneously detect RF of IgG and IgA subclasses in addition to RF IgM and are not susceptible to prozone. It has become apparent that the specificity and predictive value of the RF test is substantially increased by the detection of all three RF isotypes.


Methodology

Enzyme-linked immunosorbent assay (ELISA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
520178 RF Isotypes IgG, IgA, IgM EIA 520100 RF, IgG by EIA (RDL) U 33314-6
520178 RF Isotypes IgG, IgA, IgM EIA 520102 RF, IgA by EIA (RDL) U 33313-8
520178 RF Isotypes IgG, IgA, IgM EIA 520098 RF, IgM by EIA (RDL) U 9338-5

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