Test Details
Methodology
Enzyme-linked immunosorbent assay (ELISA)
Result Turnaround Time
14 - 21 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
The tests in this profile are not Rheumatoid Factor by Turbidimetry.
Use
EIA methods have the added advantage of being able to simultaneously detect RF of IgG and IgA subclasses in addition to RF IgM and are not susceptible to prozone. It has become apparent that the specificity and predictive value of the RF test is substantially increased by the detection of all three RF isotypes.
Specimen Requirements
Specimen
Serum
Volume
4 mL
Minimum Volume
3 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 7 days |
Refrigerated | 14 days |
Frozen | 60 days |
Freeze/thaw cycles | Stable x1 |
Storage Instructions
Refrigerate.
Causes for Rejection
Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 520178 | RF Isotypes IgG, IgA, IgM EIA | 520100 | RF, IgG by EIA (RDL) | U | 33314-6 | |
| 520178 | RF Isotypes IgG, IgA, IgM EIA | 520102 | RF, IgA by EIA (RDL) | U | 33313-8 | |
| 520178 | RF Isotypes IgG, IgA, IgM EIA | 520098 | RF, IgM by EIA (RDL) | U | 9338-5 | |
| Order Code | 520178 | |||||
| Order Code Name | RF Isotypes IgG, IgA, IgM EIA | |||||
| Order Loinc | ||||||
| Result Code | 520100 | |||||
| Result Code Name | RF, IgG by EIA (RDL) | |||||
| UofM | U | |||||
| Result LOINC | 33314-6 | |||||
| Order Code | 520178 | |||||
| Order Code Name | RF Isotypes IgG, IgA, IgM EIA | |||||
| Order Loinc | ||||||
| Result Code | 520102 | |||||
| Result Code Name | RF, IgA by EIA (RDL) | |||||
| UofM | U | |||||
| Result LOINC | 33313-8 | |||||
| Order Code | 520178 | |||||
| Order Code Name | RF Isotypes IgG, IgA, IgM EIA | |||||
| Order Loinc | ||||||
| Result Code | 520098 | |||||
| Result Code Name | RF, IgM by EIA (RDL) | |||||
| UofM | U | |||||
| Result LOINC | 9338-5 |