Test Details
Methodology
Enzyme-linked immunosorbent assay (ELISA)
Result Turnaround Time
14 - 21 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
The LKM-1 reactivity is characterized by staining of the hepatocyte cytoplasm and the proximal, but not the distal kidney tubules. Patients with AIH, type 2a disease tend to be young, female, have severe disease, have low IgA levels, have a good response to immunosuppressive therapy and are hepatitis C virus (HCV) negative. The major target antigen of LKM-1 antibodies has been identified as cytochrome P450 2D6, a microsomal protein found in the endoplasmic reticulum. LKM-1 antibodies have been reported in up to 8% of patients with chronic HCV infection.
Specimen Requirements
Specimen
Serum
Volume
1 mL
Minimum Volume
0.3 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Collection Instructions
Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 7 days |
Refrigerated | 14 days |
Frozen | 60 days |
Freeze/thaw cycles | Stable x1 |
Storage Instructions
Refrigerate or freeze.
Causes for Rejection
Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 520087 | Anti-Liver/Kidney Ab (RDL) | 32220-6 | 520116 | Anti-Liver/Kidney Ab (RDL) | Units | 32220-6 |
| Order Code | 520087 | |||||
| Order Code Name | Anti-Liver/Kidney Ab (RDL) | |||||
| Order Loinc | 32220-6 | |||||
| Result Code | 520116 | |||||
| Result Code Name | Anti-Liver/Kidney Ab (RDL) | |||||
| UofM | Units | |||||
| Result LOINC | 32220-6 |