505750: T-Cell Activation Profile, CD8 Subsets

Test Details

Methodology

Flow cytometry

Result Turnaround Time

1 - 3 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Test Includes

Percentage and absolute values CD3⁺, CD3⁺CD4⁺, CD3⁺CD8⁺, CD3⁺CD25⁺, CD8⁺CD38⁺, CD8⁺HLA-DR⁺, CD4:CD8 ratio; CBC with differential

Use

This test is an expanded profile for AIDS monitoring and prognosis. An increase in activation markers is associated with poor prognosis.

Limitations

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

References

Landay AL, Jessop C, Lennette ET, Levy JA. Chronic fatigue syndrome: Clinical condition associated with immune activation. Lancet. 1991 Sep 21; 338(8769):707-712. 1679864

Sadler D, Keren DF. Surface marker assays in immunodeficiency diseases. In: Keren DF, Hanson CA, Hurtubise PE, eds. Flow Cytometry and Clinical Diseases. Chicago, Ill: ASCP Press;1994:chap 9.

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection Instructions

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Stability Requirements

Temperature	Period
Room temperature	Yellow-top (ACD-A) or (ACD-B) tube: 2 days Lavender-top (EDTA) tube: 1 day
Refrigerated	Unstable
Frozen	Unstable
Freeze/thaw cycles	Unstable

Reference Range

Reference intervals are established by the laboratory. See table.

CD	95% Confidence Interval
	Percentage		Cells/mm³
	Minimum	Maximum	Minimum	Maximum
CD3⁺	57.5	86.2	622	2402
CD3⁺CD4⁺	30.8	58.5	359	1519
CD3⁺CD8⁺	12.0	35.5	109	897
CD3⁺CD25⁺	4.9	25.9	79	535
T4:T8 ratio	0.92	3.72
CD8⁺CD38⁺	0	17.7	0	381
CD8⁺HLA-DR⁺	0	4.9	0	117

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

LOINC® Map

Order Code	Order Code Name	Order Loinc	Result Code	Result Code Name	UofM	Result LOINC
505750	T-Cell Activation, CD8 Subsets	65758-5	505350	Absolute CD 3	/uL	8122-4
505750	T-Cell Activation, CD8 Subsets	65758-5	505324	% CD 3 Pos. Lymph.	%	8124-0
505750	T-Cell Activation, CD8 Subsets	65758-5	505351	Absolute CD 4 Helper	/uL	24467-3
505750	T-Cell Activation, CD8 Subsets	65758-5	505326	% CD 4 Pos. Lymph.	%	8123-2
505750	T-Cell Activation, CD8 Subsets	65758-5	505352	Absolute CD 8 (Supp)	/uL	14135-8
505750	T-Cell Activation, CD8 Subsets	65758-5	505328	% CD 8 Pos. Lymph.	%	8101-8
505750	T-Cell Activation, CD8 Subsets	65758-5	505333	CD4/CD8 Ratio		54218-3
505750	T-Cell Activation, CD8 Subsets	65758-5	505162	Abs.CD8+HLA-DR+Lymph	/uL	16107-5
505750	T-Cell Activation, CD8 Subsets	65758-5	505161	% CD8+HLA-DR+ Lymphs	%	13337-1
505750	T-Cell Activation, CD8 Subsets	65758-5	505157	% CD3+CD25+ Lymphs	%	13333-0
505750	T-Cell Activation, CD8 Subsets	65758-5	505158	Abs.CD3+CD25+ Lymphs	/uL	26997-7
505750	T-Cell Activation, CD8 Subsets	65758-5	505151	% CD8+CD38+ Lymphs	%	13344-7
505750	T-Cell Activation, CD8 Subsets	65758-5	505153	Abs.CD8+CD38+ Lymphs	/uL	21374-4
505750	T-Cell Activation, CD8 Subsets	65758-5	005025	WBC	x10E3/uL	6690-2
505750	T-Cell Activation, CD8 Subsets	65758-5	005033	RBC	x10E6/uL	789-8
505750	T-Cell Activation, CD8 Subsets	65758-5	005041	Hemoglobin	g/dL	718-7
505750	T-Cell Activation, CD8 Subsets	65758-5	005058	Hematocrit	%	4544-3
505750	T-Cell Activation, CD8 Subsets	65758-5	015065	MCV	fL	787-2
505750	T-Cell Activation, CD8 Subsets	65758-5	015073	MCH	pg	785-6
505750	T-Cell Activation, CD8 Subsets	65758-5	015081	MCHC	g/dL	786-4
505750	T-Cell Activation, CD8 Subsets	65758-5	105007	RDW	%	788-0
505750	T-Cell Activation, CD8 Subsets	65758-5	015172	Platelets	x10E3/uL	777-3
505750	T-Cell Activation, CD8 Subsets	65758-5	015107	Neutrophils	%	770-8
505750	T-Cell Activation, CD8 Subsets	65758-5	015123	Lymphs	%	736-9
505750	T-Cell Activation, CD8 Subsets	65758-5	015131	Monocytes	%	5905-5
505750	T-Cell Activation, CD8 Subsets	65758-5	015149	Eos	%	713-8
505750	T-Cell Activation, CD8 Subsets	65758-5	015156	Basos	%	706-2
505750	T-Cell Activation, CD8 Subsets	65758-5	115398	Immature Cells		N/A
505750	T-Cell Activation, CD8 Subsets	65758-5	015909	Neutrophils (Absolute)	x10E3/uL	751-8
505750	T-Cell Activation, CD8 Subsets	65758-5	015917	Lymphs (Absolute)	x10E3/uL	731-0
505750	T-Cell Activation, CD8 Subsets	65758-5	015925	Monocytes(Absolute)	x10E3/uL	742-7
505750	T-Cell Activation, CD8 Subsets	65758-5	015933	Eos (Absolute)	x10E3/uL	711-2
505750	T-Cell Activation, CD8 Subsets	65758-5	015941	Baso (Absolute)	x10E3/uL	704-7
505750	T-Cell Activation, CD8 Subsets	65758-5	015108	Immature Granulocytes	%	71695-1
505750	T-Cell Activation, CD8 Subsets	65758-5	015911	Immature Grans (Abs)	x10E3/uL	53115-2
505750	T-Cell Activation, CD8 Subsets	65758-5	015945	NRBC	%	58413-6
505750	T-Cell Activation, CD8 Subsets	65758-5	015180	Hematology Comments:		18314-5
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505350
Result Code Name	Absolute CD 3
UofM	/uL
Result LOINC	8122-4
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505324
Result Code Name	% CD 3 Pos. Lymph.
UofM	%
Result LOINC	8124-0
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505351
Result Code Name	Absolute CD 4 Helper
UofM	/uL
Result LOINC	24467-3
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505326
Result Code Name	% CD 4 Pos. Lymph.
UofM	%
Result LOINC	8123-2
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505352
Result Code Name	Absolute CD 8 (Supp)
UofM	/uL
Result LOINC	14135-8
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505328
Result Code Name	% CD 8 Pos. Lymph.
UofM	%
Result LOINC	8101-8
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505333
Result Code Name	CD4/CD8 Ratio
UofM
Result LOINC	54218-3
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505162
Result Code Name	Abs.CD8+HLA-DR+Lymph
UofM	/uL
Result LOINC	16107-5
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505161
Result Code Name	% CD8+HLA-DR+ Lymphs
UofM	%
Result LOINC	13337-1
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505157
Result Code Name	% CD3+CD25+ Lymphs
UofM	%
Result LOINC	13333-0
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505158
Result Code Name	Abs.CD3+CD25+ Lymphs
UofM	/uL
Result LOINC	26997-7
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505151
Result Code Name	% CD8+CD38+ Lymphs
UofM	%
Result LOINC	13344-7
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	505153
Result Code Name	Abs.CD8+CD38+ Lymphs
UofM	/uL
Result LOINC	21374-4
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	005025
Result Code Name	WBC
UofM	x10E3/uL
Result LOINC	6690-2
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	005033
Result Code Name	RBC
UofM	x10E6/uL
Result LOINC	789-8
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	005041
Result Code Name	Hemoglobin
UofM	g/dL
Result LOINC	718-7
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	005058
Result Code Name	Hematocrit
UofM	%
Result LOINC	4544-3
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015065
Result Code Name	MCV
UofM	fL
Result LOINC	787-2
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015073
Result Code Name	MCH
UofM	pg
Result LOINC	785-6
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015081
Result Code Name	MCHC
UofM	g/dL
Result LOINC	786-4
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	105007
Result Code Name	RDW
UofM	%
Result LOINC	788-0
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015172
Result Code Name	Platelets
UofM	x10E3/uL
Result LOINC	777-3
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015107
Result Code Name	Neutrophils
UofM	%
Result LOINC	770-8
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015123
Result Code Name	Lymphs
UofM	%
Result LOINC	736-9
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015131
Result Code Name	Monocytes
UofM	%
Result LOINC	5905-5
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015149
Result Code Name	Eos
UofM	%
Result LOINC	713-8
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015156
Result Code Name	Basos
UofM	%
Result LOINC	706-2
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	115398
Result Code Name	Immature Cells
UofM
Result LOINC	N/A
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015909
Result Code Name	Neutrophils (Absolute)
UofM	x10E3/uL
Result LOINC	751-8
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015917
Result Code Name	Lymphs (Absolute)
UofM	x10E3/uL
Result LOINC	731-0
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015925
Result Code Name	Monocytes(Absolute)
UofM	x10E3/uL
Result LOINC	742-7
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015933
Result Code Name	Eos (Absolute)
UofM	x10E3/uL
Result LOINC	711-2
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015941
Result Code Name	Baso (Absolute)
UofM	x10E3/uL
Result LOINC	704-7
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015108
Result Code Name	Immature Granulocytes
UofM	%
Result LOINC	71695-1
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015911
Result Code Name	Immature Grans (Abs)
UofM	x10E3/uL
Result LOINC	53115-2
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015945
Result Code Name	NRBC
UofM	%
Result LOINC	58413-6
Order Code	505750
Order Code Name	T-Cell Activation, CD8 Subsets
Order Loinc	65758-5
Result Code	015180
Result Code Name	Hematology Comments:
UofM
Result LOINC	18314-5

Reflex Table for Immature Cells
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	115399	Immature Cells	115400	Bands	%	35332-6
Reflex 1
Order Code	115399
Order Name	Immature Cells
Result Code	115400
Result Name	Bands
UofM	%
Result LOINC	35332-6

Reflex Table for Immature Cells
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	115399	Immature Cells	115401	Metamyelocytes	%	28541-1
Reflex 1
Order Code	115399
Order Name	Immature Cells
Result Code	115401
Result Name	Metamyelocytes
UofM	%
Result LOINC	28541-1

Reflex Table for Immature Cells
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	115399	Immature Cells	115403	Myelocytes	%	26498-6
Reflex 1
Order Code	115399
Order Name	Immature Cells
Result Code	115403
Result Name	Myelocytes
UofM	%
Result LOINC	26498-6

Reflex Table for Immature Cells
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	115399	Immature Cells	115404	Promyelocytes	%	26498-6
Reflex 1
Order Code	115399
Order Name	Immature Cells
Result Code	115404
Result Name	Promyelocytes
UofM	%
Result LOINC	26498-6

Reflex Table for Immature Cells
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	115399	Immature Cells	115405	Blasts/blast like cells	%	26498-6
Reflex 1
Order Code	115399
Order Name	Immature Cells
Result Code	115405
Result Name	Blasts/blast like cells
UofM	%
Result LOINC	26498-6

Reflex Table for Immature Cells
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	115399	Immature Cells	115406	Megakaryocytes	%	19252-6
Reflex 1
Order Code	115399
Order Name	Immature Cells
Result Code	115406
Result Name	Megakaryocytes
UofM	%
Result LOINC	19252-6

Reflex Table for Immature Cells
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	115399	Immature Cells	115265	Other, Lineage Uncertain	%	55433-7
Reflex 1
Order Code	115399
Order Name	Immature Cells
Result Code	115265
Result Name	Other, Lineage Uncertain
UofM	%
Result LOINC	55433-7

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