Test Details
Methodology
Flow cytometry
Result Turnaround Time
1 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Percentage CD4+; absolute CD4+; percentage CD8+; absolute CD8+; CD4:CD8 ratio; CBC with differential
References
Custom Additional Information
HIV-1 infection results in a decrease of CD4 T cells, an increase of CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. In HIV-1 seropositive patients, enumeration of CD4 T cells may be used for prognostic purposes and to monitor disease progression and antiretroviral therapy.
Specimen Requirements
Specimen
Whole blood
Volume
Fill tube(s) to capacity.
Container
Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube
Collection Instructions
Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.
Stability Requirements
Temperature | Period |
---|---|
Room temperature | Yellow-top (ACD-A) or (ACD-B) tube: 2 days Lavender-top (EDTA) tube: 1 day |
Refrigerated | Unstable |
Frozen | Unstable |
Freeze/thaw cycles | Unstable |
Reference Range
Reference intervals for adults have been established by the laboratory. See table.
CD | Age | 95% Confidence Interval | |||
---|---|---|---|---|---|
Percentage | Cells/mm3 | ||||
Minimum | Maximum | Minimum | Maximum | ||
Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393. | |||||
CD4+ | Neonates | 17 | 52 | 400 | 3500 |
1 wk to 2 m | 41 | 68 | 1700 | 5300 | |
2 to 5 m | 33 | 58 | 1500 | 5000 | |
5 to 9 m | 33 | 58 | 1400 | 5100 | |
9 to 15 m | 31 | 54 | 1000 | 4600 | |
15 to 24 m | 25 | 50 | 900 | 5500 | |
2 to 5 y | 23 | 48 | 500 | 2400 | |
5 to 10 y | 27 | 53 | 300 | 2000 | |
10 to 16 y | 25 | 48 | 400 | 2100 | |
Adults | 31 | 59 | 359 | 1519 | |
CD8+ | Neonates | 10 | 41 | 200 | 1900 |
1 wk to 2 m | 9 | 23 | 400 | 1700 | |
2 to 5 m | 11 | 25 | 500 | 1600 | |
5 to 9 m | 13 | 26 | 600 | 2200 | |
9 to 15 m | 12 | 28 | 400 | 2100 | |
15 to 24 m | 11 | 32 | 400 | 2300 | |
2 to 5 y | 14 | 33 | 300 | 1600 | |
5 to 10 y | 19 | 34 | 300 | 1800 | |
10 to 16 y | 9 | 35 | 200 | 1200 | |
Adults | 12 | 36 | 109 | 897 | |
T4:T8 ratio | Adults | 0.92 | 3.72 |
Storage Instructions
Maintain specimen at room temperature.
Causes for Rejection
Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen
LOINC® Map
Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
---|---|---|---|---|---|---|
505271 | CD4/CD8 Ratio Profile | 65758-5 | 505010 | Absolute CD 4 Helper | /uL | 24467-3 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 505009 | % CD 4 Pos. Lymph. | % | 8123-2 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 505579 | Abs. CD 8 Suppressor | /uL | 14135-8 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 505580 | % CD 8 Pos. Lymph. | % | 8101-8 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 505273 | CD4/CD8 Ratio | 54218-3 | |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 005025 | WBC | x10E3/uL | 6690-2 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 005033 | RBC | x10E6/uL | 789-8 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 005041 | Hemoglobin | g/dL | 718-7 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 005058 | Hematocrit | % | 4544-3 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015065 | MCV | fL | 787-2 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015073 | MCH | pg | 785-6 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015081 | MCHC | g/dL | 786-4 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 105007 | RDW | % | 788-0 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015172 | Platelets | x10E3/uL | 777-3 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015107 | Neutrophils | % | 770-8 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015123 | Lymphs | % | 736-9 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015131 | Monocytes | % | 5905-5 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015149 | Eos | % | 713-8 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015156 | Basos | % | 706-2 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 115398 | Immature Cells | N/A | |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015909 | Neutrophils (Absolute) | x10E3/uL | 751-8 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015917 | Lymphs (Absolute) | x10E3/uL | 731-0 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015925 | Monocytes(Absolute) | x10E3/uL | 742-7 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015933 | Eos (Absolute) | x10E3/uL | 711-2 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015941 | Baso (Absolute) | x10E3/uL | 704-7 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015108 | Immature Granulocytes | % | 71695-1 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015911 | Immature Grans (Abs) | x10E3/uL | 53115-2 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015945 | NRBC | % | 58413-6 |
505271 | CD4/CD8 Ratio Profile | 65758-5 | 015180 | Hematology Comments: | 18314-5 | |
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 505010 | |||||
Result Code Name | Absolute CD 4 Helper | |||||
UofM | /uL | |||||
Result LOINC | 24467-3 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 505009 | |||||
Result Code Name | % CD 4 Pos. Lymph. | |||||
UofM | % | |||||
Result LOINC | 8123-2 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 505579 | |||||
Result Code Name | Abs. CD 8 Suppressor | |||||
UofM | /uL | |||||
Result LOINC | 14135-8 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 505580 | |||||
Result Code Name | % CD 8 Pos. Lymph. | |||||
UofM | % | |||||
Result LOINC | 8101-8 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 505273 | |||||
Result Code Name | CD4/CD8 Ratio | |||||
UofM | ||||||
Result LOINC | 54218-3 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 005025 | |||||
Result Code Name | WBC | |||||
UofM | x10E3/uL | |||||
Result LOINC | 6690-2 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 005033 | |||||
Result Code Name | RBC | |||||
UofM | x10E6/uL | |||||
Result LOINC | 789-8 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 005041 | |||||
Result Code Name | Hemoglobin | |||||
UofM | g/dL | |||||
Result LOINC | 718-7 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 005058 | |||||
Result Code Name | Hematocrit | |||||
UofM | % | |||||
Result LOINC | 4544-3 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015065 | |||||
Result Code Name | MCV | |||||
UofM | fL | |||||
Result LOINC | 787-2 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015073 | |||||
Result Code Name | MCH | |||||
UofM | pg | |||||
Result LOINC | 785-6 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015081 | |||||
Result Code Name | MCHC | |||||
UofM | g/dL | |||||
Result LOINC | 786-4 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 105007 | |||||
Result Code Name | RDW | |||||
UofM | % | |||||
Result LOINC | 788-0 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015172 | |||||
Result Code Name | Platelets | |||||
UofM | x10E3/uL | |||||
Result LOINC | 777-3 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015107 | |||||
Result Code Name | Neutrophils | |||||
UofM | % | |||||
Result LOINC | 770-8 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015123 | |||||
Result Code Name | Lymphs | |||||
UofM | % | |||||
Result LOINC | 736-9 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015131 | |||||
Result Code Name | Monocytes | |||||
UofM | % | |||||
Result LOINC | 5905-5 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015149 | |||||
Result Code Name | Eos | |||||
UofM | % | |||||
Result LOINC | 713-8 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015156 | |||||
Result Code Name | Basos | |||||
UofM | % | |||||
Result LOINC | 706-2 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 115398 | |||||
Result Code Name | Immature Cells | |||||
UofM | ||||||
Result LOINC | N/A | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015909 | |||||
Result Code Name | Neutrophils (Absolute) | |||||
UofM | x10E3/uL | |||||
Result LOINC | 751-8 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015917 | |||||
Result Code Name | Lymphs (Absolute) | |||||
UofM | x10E3/uL | |||||
Result LOINC | 731-0 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015925 | |||||
Result Code Name | Monocytes(Absolute) | |||||
UofM | x10E3/uL | |||||
Result LOINC | 742-7 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015933 | |||||
Result Code Name | Eos (Absolute) | |||||
UofM | x10E3/uL | |||||
Result LOINC | 711-2 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015941 | |||||
Result Code Name | Baso (Absolute) | |||||
UofM | x10E3/uL | |||||
Result LOINC | 704-7 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015108 | |||||
Result Code Name | Immature Granulocytes | |||||
UofM | % | |||||
Result LOINC | 71695-1 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015911 | |||||
Result Code Name | Immature Grans (Abs) | |||||
UofM | x10E3/uL | |||||
Result LOINC | 53115-2 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015945 | |||||
Result Code Name | NRBC | |||||
UofM | % | |||||
Result LOINC | 58413-6 | |||||
Order Code | 505271 | |||||
Order Code Name | CD4/CD8 Ratio Profile | |||||
Order Loinc | 65758-5 | |||||
Result Code | 015180 | |||||
Result Code Name | Hematology Comments: | |||||
UofM | ||||||
Result LOINC | 18314-5 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 115399 | Immature Cells | 115400 | Bands | % | 35332-6 |
Reflex 1 | ||||||
Order Code | 115399 | |||||
Order Name | Immature Cells | |||||
Result Code | 115400 | |||||
Result Name | Bands | |||||
UofM | % | |||||
Result LOINC | 35332-6 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 115399 | Immature Cells | 115401 | Metamyelocytes | % | 28541-1 |
Reflex 1 | ||||||
Order Code | 115399 | |||||
Order Name | Immature Cells | |||||
Result Code | 115401 | |||||
Result Name | Metamyelocytes | |||||
UofM | % | |||||
Result LOINC | 28541-1 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 115399 | Immature Cells | 115403 | Myelocytes | % | 26498-6 |
Reflex 1 | ||||||
Order Code | 115399 | |||||
Order Name | Immature Cells | |||||
Result Code | 115403 | |||||
Result Name | Myelocytes | |||||
UofM | % | |||||
Result LOINC | 26498-6 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 115399 | Immature Cells | 115404 | Promyelocytes | % | 26498-6 |
Reflex 1 | ||||||
Order Code | 115399 | |||||
Order Name | Immature Cells | |||||
Result Code | 115404 | |||||
Result Name | Promyelocytes | |||||
UofM | % | |||||
Result LOINC | 26498-6 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 115399 | Immature Cells | 115405 | Blasts/blast like cells | % | 26498-6 |
Reflex 1 | ||||||
Order Code | 115399 | |||||
Order Name | Immature Cells | |||||
Result Code | 115405 | |||||
Result Name | Blasts/blast like cells | |||||
UofM | % | |||||
Result LOINC | 26498-6 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 115399 | Immature Cells | 115406 | Megakaryocytes | % | 19252-6 |
Reflex 1 | ||||||
Order Code | 115399 | |||||
Order Name | Immature Cells | |||||
Result Code | 115406 | |||||
Result Name | Megakaryocytes | |||||
UofM | % | |||||
Result LOINC | 19252-6 |
Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
---|---|---|---|---|---|---|
Reflex 1 | 115399 | Immature Cells | 115265 | Other, Lineage Uncertain | % | 55433-7 |
Reflex 1 | ||||||
Order Code | 115399 | |||||
Order Name | Immature Cells | |||||
Result Code | 115265 | |||||
Result Name | Other, Lineage Uncertain | |||||
UofM | % | |||||
Result LOINC | 55433-7 |