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Natural Killer Cell Surface Antigen (CD3-CD56+ Marker Analysis)
CPT:
86357
Test Includes
Percentage CD3-CD56+ natural killers (NK+); absolute CD3-CD56+ natural killers (NK+); absolute lymphocyte count
Expected Turnaround Time
1 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Specimen Requirements
Specimen
Whole blood
Volume
Fill tube(s) to capacity.
Container
Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube
Collection
Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.
Storage Instructions
Maintain specimen at room temperature.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | Yellow-top (ACD-A) or (ACD-B) tube: 2 days Lavender-top (EDTA) tube: 1 day |
Refrigerated | Unstable |
Frozen | Unstable |
Freeze/thaw cycles | Unstable |
Causes for Rejection
Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen
Test Details
Use
This test is used to determine levels of natural killer cells in circulation and monitor immune stimulation therapy.
Methodology
Flow cytometry
Reference Interval
Reference intervals for adults have been established by the laboratory. See table.
Age | 95% Confidence Interval | |||
|---|---|---|---|---|
Percentage | Cells/mm3 | |||
Minimum | Maximum | Minimum | Maximum | |
Pediatric reference intervals are from Comans-Bitter WM, de Groot R, van den Beemd R, et al. Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatrics. 1997 Mar; 130(3):388-393. | ||||
Neonates | 6 | 58 | 100 | 1900 |
1 wk to 2 m | 3 | 23 | 200 | 1400 |
2 to 5 m | 2 | 14 | 100 | 1300 |
5 to 9 m | 2 | 13 | 100 | 1000 |
9 to 15 m | 3 | 17 | 200 | 1200 |
15 to 24 m | 3 | 16 | 100 | 1400 |
2 to 5 y | 4 | 23 | 100 | 1000 |
5 to 10 y | 4 | 26 | 90 | 900 |
10 to 16 y | 6 | 27 | 70 | 1200 |
Adults | 1 | 19 | 24 | 406 |
Additional Information
Natural killer (NK) cells are large granular lymphocytes that mediate MHC-unrestricted cytotoxicity against virus-infected and malignant cells and manufacture a number of cytokines following stimulation of the immune system.
References
Centers for Disease Control and Prevention (CDC). 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV).MMWR. 1997 Jan 10; 46:RR-2.
Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 505016 | Natural Killer Cell Surface Ag | 506730 | Ab NK (CD56/16) | /uL | 9728-7 | |
| 505016 | Natural Killer Cell Surface Ag | 506729 | % NK (CD56/16) | % | 8112-5 | |
| 505016 | Natural Killer Cell Surface Ag | 005025 | WBC | x10E3/uL | 6690-2 | |
| 505016 | Natural Killer Cell Surface Ag | 005033 | RBC | x10E6/uL | 789-8 | |
| 505016 | Natural Killer Cell Surface Ag | 005041 | Hemoglobin | g/dL | 718-7 | |
| 505016 | Natural Killer Cell Surface Ag | 005058 | Hematocrit | % | 4544-3 | |
| 505016 | Natural Killer Cell Surface Ag | 015065 | MCV | fL | 787-2 | |
| 505016 | Natural Killer Cell Surface Ag | 015073 | MCH | pg | 785-6 | |
| 505016 | Natural Killer Cell Surface Ag | 015081 | MCHC | g/dL | 786-4 | |
| 505016 | Natural Killer Cell Surface Ag | 105007 | RDW | % | 788-0 | |
| 505016 | Natural Killer Cell Surface Ag | 015172 | Platelets | x10E3/uL | 777-3 | |
| 505016 | Natural Killer Cell Surface Ag | 015107 | Neutrophils | % | 770-8 | |
| 505016 | Natural Killer Cell Surface Ag | 015123 | Lymphs | % | 736-9 | |
| 505016 | Natural Killer Cell Surface Ag | 015131 | Monocytes | % | 5905-5 | |
| 505016 | Natural Killer Cell Surface Ag | 015149 | Eos | % | 713-8 | |
| 505016 | Natural Killer Cell Surface Ag | 015156 | Basos | % | 706-2 | |
| 505016 | Natural Killer Cell Surface Ag | 115398 | Immature Cells | N/A | ||
| 505016 | Natural Killer Cell Surface Ag | 015909 | Neutrophils (Absolute) | x10E3/uL | 751-8 | |
| 505016 | Natural Killer Cell Surface Ag | 015917 | Lymphs (Absolute) | x10E3/uL | 731-0 | |
| 505016 | Natural Killer Cell Surface Ag | 015925 | Monocytes(Absolute) | x10E3/uL | 742-7 | |
| 505016 | Natural Killer Cell Surface Ag | 015933 | Eos (Absolute) | x10E3/uL | 711-2 | |
| 505016 | Natural Killer Cell Surface Ag | 015941 | Baso (Absolute) | x10E3/uL | 704-7 | |
| 505016 | Natural Killer Cell Surface Ag | 015108 | Immature Granulocytes | % | 71695-1 | |
| 505016 | Natural Killer Cell Surface Ag | 015911 | Immature Grans (Abs) | x10E3/uL | 53115-2 | |
| 505016 | Natural Killer Cell Surface Ag | 015945 | NRBC | % | 58413-6 | |
| 505016 | Natural Killer Cell Surface Ag | 015180 | Hematology Comments: | 18314-5 |
| Reflex Table for Immature Cells | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 115399 | Immature Cells | 115400 | Bands | % | 35332-6 |
| Reflex Table for Immature Cells | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 115399 | Immature Cells | 115401 | Metamyelocytes | % | 28541-1 |
| Reflex Table for Immature Cells | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 115399 | Immature Cells | 115403 | Myelocytes | % | 26498-6 |
| Reflex Table for Immature Cells | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 115399 | Immature Cells | 115404 | Promyelocytes | % | 26498-6 |
| Reflex Table for Immature Cells | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 115399 | Immature Cells | 115405 | Blasts/blast like cells | % | 26498-6 |
| Reflex Table for Immature Cells | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 115399 | Immature Cells | 115406 | Megakaryocytes | % | 19252-6 |
| Reflex Table for Immature Cells | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 115399 | Immature Cells | 115265 | Other, Lineage Uncertain | % | 55433-7 |
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