Dihydrotestosterone (DHT), Free, LC/MS/Dialysis

TEST: 504026
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CPT: 84999
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Synonyms

  • Free 5-α-dihydrotestoterone

Expected Turnaround Time

9 - 15 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements

Specimen

Serum (preferred) or plasma, frozen

Volume

3 mL

Minimum Volume

1.2 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube, gel-barrier tube, or green-top (heparin) tube

Collection

Separate serum or plasma from cells within four hours of collection. Send serum or plasma in a plastic transport tube. Transfer specimen to a plastic transport tube before freezing. To avoid delays in turnaround time when requesting multiple test on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze. Stable at room temperature, refrigerated, or frozen for 14 days. Freeze/thaw cycles: stable x6

Causes for Rejection

EDTA plasma; grossly hemolyzed, icteric, or lipemic sample

Test Details

Use

Dihydrotestosterone measurement is used to diagnose 5α-reductase deficiency and for the evaluation of androgen utilization. Dihydrotestosterone is produced by the reduction of testosterone by 5α-reductase in the target organs. Circulating dihydrotestosterone is tightly bound to sex hormone-binding globulin. The free fraction represents the dihydrotestosterone available to act on tissues.

Limitations

This test was developed, and the performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

Methodology

Equilibrium dialysis, high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS-MS)

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
504026 DHT, Free, LCMS/Dialysis 500143 Dihydrotestosterone ng/dL 1848-1
504026 DHT, Free, LCMS/Dialysis 504028 DHT, Percent Free Dialysis % 13882-6
504026 DHT, Free, LCMS/Dialysis 504029 DHT, Free pg/mL 14047-5

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