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Progesterone, Free, With Total Progesterone, LC/MS-MS (Endocrine Sciences)

CPT 84144; 84999

Test Details

Methodology

High-pressure liquid chromatography (HPLC)/tandem mass spectrometry, equilibrium dialysis, calculation

Result Turnaround Time

8 - 12 days

Use

Progesterone plays an important role in the secretory transformation of the endometrium, maintenance of gestation, and relaxation of the myometrium. Progesterone is also a marker for ovulation and luteal phase of the menstrual cycle. Steroid hormones (like progesterone) circulate in the blood in protein-bound and free forms, and it is the free form that exhibits biological activity. The free form of progesterone is expected to reflect the biological activity of progesterone.

Specimen Requirements

Limitations

The test is not accurate for hemolyzed samples. Cortisol and other hormones that bind transcortin (corticosteroid-binding globulin) may affect the percentage of free progesterone.

Results of this test are for investigational purposes only. The performance characteristics of this assay have been determined by LabCorp. The result should not be used as a diagnostic procedure without confirmation of the diagnosis by another medically established diagnostic product or procedure.

References

Batra S, Bengtsson IP, Grundsell H, Sjöberg NO. Levels of free and protein-bound progesterone in plasma during late pregnancy. J Clin Endocrinol Metab. 1976 Jun; 42(6):1041-1047. 932171
Shintani K, Yoshida N, Sekiba K. Changes in free progesterone in the serum, peritoneal fluid and follicular fluid through the menstrual cycle and pregnancy. Endocrinol Jpn. 1987 Apr; 34(2):221-230. 3622390

Specimen

Serum (preferred) or plasma

Volume

1.6 mL

Minimum Volume

0.8 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (sodium heparin) tube

Storage Instructions

Freeze (preferred) or refrigerate.

Collection Instructions

To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Stability Requirements

Temperature

Period

Room temperature

6 days

Refrigerated

6 days

Frozen

6 days

Freeze/thaw cycles

Stable x6

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
503658 Total and Free Progesterone 500168 Progesterone, Serum ng/dL 2839-9
503658 Total and Free Progesterone 503661 % Free Progesterone (Dialysis) % 72890-7
503658 Total and Free Progesterone 503659 Free Progesterone ng/dL 72889-9
Order Code503658
Order Code NameTotal and Free Progesterone
Order Loinc
Result Code500168
Result Code NameProgesterone, Serum
UofMng/dL
Result LOINC2839-9
Order Code503658
Order Code NameTotal and Free Progesterone
Order Loinc
Result Code503661
Result Code Name% Free Progesterone (Dialysis)
UofM%
Result LOINC72890-7
Order Code503658
Order Code NameTotal and Free Progesterone
Order Loinc
Result Code503659
Result Code NameFree Progesterone
UofMng/dL
Result LOINC72889-9