Progesterone, Free, With Total Progesterone, LC/MS-MS (Endocrine Sciences)

TEST: 503658
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CPT: 84144; 84999
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Expected Turnaround Time

8 - 12 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements

Specimen

Serum (preferred) or plasma

Volume

1.6 mL

Minimum Volume

0.8 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (sodium heparin) tube

Collection

To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Freeze (preferred) or refrigerate.

Stability Requirements

Temperature

Period

Room temperature

6 days

Refrigerated

6 days

Frozen

6 days

Freeze/thaw cycles

Stable x6

Test Details

Use

Progesterone plays an important role in the secretory transformation of the endometrium, maintenance of gestation, and relaxation of the myometrium. Progesterone is also a marker for ovulation and luteal phase of the menstrual cycle. Steroid hormones (like progesterone) circulate in the blood in protein-bound and free forms, and it is the free form that exhibits biological activity. The free form of progesterone is expected to reflect the biological activity of progesterone.

Limitations

The test is not accurate for hemolyzed samples. Cortisol and other hormones that bind transcortin (corticosteroid-binding globulin) may affect the percentage of free progesterone.

Results of this test are for investigational purposes only. The performance characteristics of this assay have been determined by LabCorp. The result should not be used as a diagnostic procedure without confirmation of the diagnosis by another medically established diagnostic product or procedure.

Methodology

High-pressure liquid chromatography (HPLC)/tandem mass spectrometry, equilibrium dialysis, calculation

References

Batra S, Bengtsson IP, Grundsell H, Sjöberg NO. Levels of free and protein-bound progesterone in plasma during late pregnancy. J Clin Endocrinol Metab. 1976 Jun; 42(6):1041-1047. 932171
Shintani K, Yoshida N, Sekiba K. Changes in free progesterone in the serum, peritoneal fluid and follicular fluid through the menstrual cycle and pregnancy. Endocrinol Jpn. 1987 Apr; 34(2):221-230. 3622390

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
503658 Total and Free Progesterone 500168 Progesterone, Serum ng/dL 2839-9
503658 Total and Free Progesterone 503661 % Free Progesterone (Dialysis) % 72890-7
503658 Total and Free Progesterone 503659 Free Progesterone ng/dL 72889-9

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