Skip to main content

Natural Killer Cell and Activated T-Cell Profile/IL-2r

Test Number 502500
Test number copied
CPT 86356; 86357; 86359
Synonyms
  • CD25
  • Interleukin-2 Receptor
  • TAC Antigen

Test Details

Methodology

Flow cytometry

Result Turnaround Time

1 - 3 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Percentage CD3+; absolute CD3+; percentage CD3+CD25+ (IL-2 receptor); absolute CD3+CD25+; percentage CD3-CD56+ (NK); total CD3-CD56+ (NK); CBC with differential; total lymphocyte count

Use

This test is used to assess changes in T-lymphocyte surface markers that may indicate immune stimulation.

Limitations

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Custom Additional Information

Natural killer (NK) cells are large granular lymphocytes that mediate MHC-unrestricted cytotoxicity against virus-infected and malignant cells and manufacture a number of cytokines following stimulation of the immune system.

The combination of IL-2 and T-cell markers can be used to assess the state of activation of T cells. NK cells are responsible for the destruction of virally infected cells, tumor cells, and antibody-dependent cellular cytotoxicity.

Specimen Requirements

Specimen

Whole blood

Volume

Fill tube(s) to capacity.

Container

Lavender-top (EDTA) tube and yellow-top (ACD-A) or (ACD-B) tube

Collection Instructions

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Stability Requirements

Temperature

Period

Room temperature

Yellow-top (ACD-A) or (ACD-B) tube: 2 days

Lavender-top (EDTA) tube: 1 day

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable

Reference Range

Reference intervals for adults have been established by the laboratory. See table.

Adult Reference Intervals for Lymphocyte Immunophenotyping

CD

95% Confidence Interval

Percentage

Cells/mm3

Minimum

Maximum

Minimum

Maximum

CD3+

58

86

622

2402

CD3+CD25+

5

26

79

535

CD3-CD56+

1

19

24

406

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

References

Caligiuri M, Murray C, Buchwald D, et al. Phenotypic and functional deficiency of natural killer cells in patients with chronic fatigue syndrome. J Immunol. 1987 Nov 15; 139(10):3306-3313. 2824604
Landay AL, Jessop C, Lennette ET, Levy JA. Chronic fatigue syndrome: Clinical condition associated with immune activation. Lancet. 1991 Sep 21; 338(8769):707-712.1679864
Lanier L, Jackson A. Monoclonal antibodies: Differentiation antigens expressed on leukocytes. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 23.
Rich RR, ed. Clinical Immunology: Principles and Practice. St Louis, Mo: Mosby-Year Book;1996:chap 87.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
502500 NK and Activated T Cells/IL2r 502546 Ab NK (CD56/16) /uL 42188-3
502500 NK and Activated T Cells/IL2r 502545 % NK (CD56/16) % 42189-1
502500 NK and Activated T Cells/IL2r 505350 Absolute CD 3 /uL 8122-4
502500 NK and Activated T Cells/IL2r 505324 % CD 3 Pos. Lymph. % 8124-0
502500 NK and Activated T Cells/IL2r 505158 Abs.CD3+CD25+ Lymphs /uL 26997-7
502500 NK and Activated T Cells/IL2r 505157 % CD3+CD25+ Lymphs % 13333-0
502500 NK and Activated T Cells/IL2r 005025 WBC x10E3/uL 6690-2
502500 NK and Activated T Cells/IL2r 005033 RBC x10E6/uL 789-8
502500 NK and Activated T Cells/IL2r 005041 Hemoglobin g/dL 718-7
502500 NK and Activated T Cells/IL2r 005058 Hematocrit % 4544-3
502500 NK and Activated T Cells/IL2r 015065 MCV fL 787-2
502500 NK and Activated T Cells/IL2r 015073 MCH pg 785-6
502500 NK and Activated T Cells/IL2r 015081 MCHC g/dL 786-4
502500 NK and Activated T Cells/IL2r 105007 RDW % 788-0
502500 NK and Activated T Cells/IL2r 015172 Platelets x10E3/uL 777-3
502500 NK and Activated T Cells/IL2r 015107 Neutrophils % 770-8
502500 NK and Activated T Cells/IL2r 015123 Lymphs % 736-9
502500 NK and Activated T Cells/IL2r 015131 Monocytes % 5905-5
502500 NK and Activated T Cells/IL2r 015149 Eos % 713-8
502500 NK and Activated T Cells/IL2r 015156 Basos % 706-2
502500 NK and Activated T Cells/IL2r 115398 Immature Cells N/A
502500 NK and Activated T Cells/IL2r 015909 Neutrophils (Absolute) x10E3/uL 751-8
502500 NK and Activated T Cells/IL2r 015917 Lymphs (Absolute) x10E3/uL 731-0
502500 NK and Activated T Cells/IL2r 015925 Monocytes(Absolute) x10E3/uL 742-7
502500 NK and Activated T Cells/IL2r 015933 Eos (Absolute) x10E3/uL 711-2
502500 NK and Activated T Cells/IL2r 015941 Baso (Absolute) x10E3/uL 704-7
502500 NK and Activated T Cells/IL2r 015108 Immature Granulocytes % 71695-1
502500 NK and Activated T Cells/IL2r 015911 Immature Grans (Abs) x10E3/uL 53115-2
502500 NK and Activated T Cells/IL2r 015945 NRBC % 58413-6
502500 NK and Activated T Cells/IL2r 015180 Hematology Comments: 18314-5
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code502546
Result Code NameAb NK (CD56/16)
UofM/uL
Result LOINC42188-3
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code502545
Result Code Name% NK (CD56/16)
UofM%
Result LOINC42189-1
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code505350
Result Code NameAbsolute CD 3
UofM/uL
Result LOINC8122-4
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code505324
Result Code Name% CD 3 Pos. Lymph.
UofM%
Result LOINC8124-0
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code505158
Result Code NameAbs.CD3+CD25+ Lymphs
UofM/uL
Result LOINC26997-7
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code505157
Result Code Name% CD3+CD25+ Lymphs
UofM%
Result LOINC13333-0
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code005025
Result Code NameWBC
UofMx10E3/uL
Result LOINC6690-2
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code005033
Result Code NameRBC
UofMx10E6/uL
Result LOINC789-8
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code005041
Result Code NameHemoglobin
UofMg/dL
Result LOINC718-7
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code005058
Result Code NameHematocrit
UofM%
Result LOINC4544-3
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015065
Result Code NameMCV
UofMfL
Result LOINC787-2
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015073
Result Code NameMCH
UofMpg
Result LOINC785-6
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015081
Result Code NameMCHC
UofMg/dL
Result LOINC786-4
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code105007
Result Code NameRDW
UofM%
Result LOINC788-0
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015172
Result Code NamePlatelets
UofMx10E3/uL
Result LOINC777-3
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015107
Result Code NameNeutrophils
UofM%
Result LOINC770-8
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015123
Result Code NameLymphs
UofM%
Result LOINC736-9
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015131
Result Code NameMonocytes
UofM%
Result LOINC5905-5
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015149
Result Code NameEos
UofM%
Result LOINC713-8
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015156
Result Code NameBasos
UofM%
Result LOINC706-2
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code115398
Result Code NameImmature Cells
UofM
Result LOINCN/A
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015909
Result Code NameNeutrophils (Absolute)
UofMx10E3/uL
Result LOINC751-8
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015917
Result Code NameLymphs (Absolute)
UofMx10E3/uL
Result LOINC731-0
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015925
Result Code NameMonocytes(Absolute)
UofMx10E3/uL
Result LOINC742-7
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015933
Result Code NameEos (Absolute)
UofMx10E3/uL
Result LOINC711-2
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015941
Result Code NameBaso (Absolute)
UofMx10E3/uL
Result LOINC704-7
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015108
Result Code NameImmature Granulocytes
UofM%
Result LOINC71695-1
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015911
Result Code NameImmature Grans (Abs)
UofMx10E3/uL
Result LOINC53115-2
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015945
Result Code NameNRBC
UofM%
Result LOINC58413-6
Order Code502500
Order Code NameNK and Activated T Cells/IL2r
Order Loinc
Result Code015180
Result Code NameHematology Comments:
UofM
Result LOINC18314-5
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115400 Bands % 35332-6
Reflex 1
Order Code115399
Order NameImmature Cells
Result Code115400
Result NameBands
UofM%
Result LOINC35332-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115401 Metamyelocytes % 28541-1
Reflex 1
Order Code115399
Order NameImmature Cells
Result Code115401
Result NameMetamyelocytes
UofM%
Result LOINC28541-1
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115403 Myelocytes % 26498-6
Reflex 1
Order Code115399
Order NameImmature Cells
Result Code115403
Result NameMyelocytes
UofM%
Result LOINC26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115404 Promyelocytes % 26498-6
Reflex 1
Order Code115399
Order NameImmature Cells
Result Code115404
Result NamePromyelocytes
UofM%
Result LOINC26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115405 Blasts/blast like cells % 26498-6
Reflex 1
Order Code115399
Order NameImmature Cells
Result Code115405
Result NameBlasts/blast like cells
UofM%
Result LOINC26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115406 Megakaryocytes % 19252-6
Reflex 1
Order Code115399
Order NameImmature Cells
Result Code115406
Result NameMegakaryocytes
UofM%
Result LOINC19252-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115265 Other, Lineage Uncertain % 55433-7
Reflex 1
Order Code115399
Order NameImmature Cells
Result Code115265
Result NameOther, Lineage Uncertain
UofM%
Result LOINC55433-7