Test Details
Methodology
Flow cytometry
Result Turnaround Time
2 - 7 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
The following markers are utilized to test neutrophils and/or monocytes: CD24, CD15, CD45, FLAER, CD64, CD14. If a PNH clone is identified, the following markers are used to test red blood cells: glycophorin A, CD59.
Special Instructions
Collect Monday through Thursday only. Specimens must arrive at Integrated Oncology—Brentwood or Phoenix—within 48 hours of collection.
Limitations
This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Specimen Requirements
Specimen
Peripheral blood
Volume
4 mL
Minimum Volume
2 mL (Note: This volume does not allow for repeat testing.)
Container
Green-top (sodium heparin) tube (preferred) or lavender-top (EDTA)
Collection Instructions
Note: Draw Monday through Thursday only. Specimens must arrive at Integrated Oncology within 48 hours of collection.
Ship Immediately. Do not freeze. Ship with a cool pak.
Storage Instructions
Refrigerate (stable at room temperature up to 48 hours; refrigerated up to 48 hours).
Causes for Rejection
Samples with less than 1,000 WBC/ul may only have RBC analysis reported; specimens received for testing greater than 48 hours old and viability less than 80%; frozen specimen; broken container; fixed tissue; bone marrow; insufficient sample; sample greater than 48 hours old.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 502251 | Flow Cytometry PNH | 502253 | Interpretation: | 33664-4 | ||
| 502251 | Flow Cytometry PNH | 502254 | Comment: | N/A | ||
| 502251 | Flow Cytometry PNH | 502255 | Specimen: | 31208-2 | ||
| 502251 | Flow Cytometry PNH | 502256 | Submitted Dx: | N/A | ||
| 502251 | Flow Cytometry PNH | 502257 | Viability: | 33194-2 | ||
| 502251 | Flow Cytometry PNH | 502259 | Cell Population | N/A | ||
| 502251 | Flow Cytometry PNH | 502260 | Granulocytes: | 53831-4 | ||
| 502251 | Flow Cytometry PNH | 502262 | Monocytes: | 60554-3 | ||
| 502251 | Flow Cytometry PNH | 502261 | Red Blood Cells: | 49031-8 | ||
| 502251 | Flow Cytometry PNH | 502263 | Representative Histograms: | N/A | ||
| 502251 | Flow Cytometry PNH | 502264 | Antibodies Performed: | N/A | ||
| 502251 | Flow Cytometry PNH | 502274 | Comment: | N/A | ||
| 502251 | Flow Cytometry PNH | 503332 | Director Review | 72486-4 | ||
| 502251 | Flow Cytometry PNH | 481841 | 80563-0 | |||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502253 | |||||
| Result Code Name | Interpretation: | |||||
| UofM | ||||||
| Result LOINC | 33664-4 | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502254 | |||||
| Result Code Name | Comment: | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502255 | |||||
| Result Code Name | Specimen: | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502256 | |||||
| Result Code Name | Submitted Dx: | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502257 | |||||
| Result Code Name | Viability: | |||||
| UofM | ||||||
| Result LOINC | 33194-2 | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502259 | |||||
| Result Code Name | Cell Population | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502260 | |||||
| Result Code Name | Granulocytes: | |||||
| UofM | ||||||
| Result LOINC | 53831-4 | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502262 | |||||
| Result Code Name | Monocytes: | |||||
| UofM | ||||||
| Result LOINC | 60554-3 | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502261 | |||||
| Result Code Name | Red Blood Cells: | |||||
| UofM | ||||||
| Result LOINC | 49031-8 | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502263 | |||||
| Result Code Name | Representative Histograms: | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502264 | |||||
| Result Code Name | Antibodies Performed: | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 502274 | |||||
| Result Code Name | Comment: | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 503332 | |||||
| Result Code Name | Director Review | |||||
| UofM | ||||||
| Result LOINC | 72486-4 | |||||
| Order Code | 502251 | |||||
| Order Code Name | Flow Cytometry PNH | |||||
| Order Loinc | ||||||
| Result Code | 481841 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 80563-0 |