Find Locations
For hours, walk-ins and appointments.Paroxysmal Nocturnal Hemoglobinuria (PNH)
CPT:
Negative: 88184; 88185(x5); 88187; Positive: 88184; 88185(x7); 88187
Synonyms
- PNH
Special Instructions
Collect Monday through Thursday only. Specimens must arrive at Integrated Oncology—Brentwood or Phoenix—within 48 hours of collection.
Expected Turnaround Time
2 - 7 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Peripheral blood
Volume
4 mL
Minimum Volume
2 mL (Note: This volume does not allow for repeat testing.)
Container
Green-top (sodium heparin) tube (preferred) or lavender-top (EDTA)
Collection
Note: Draw Monday through Thursday only. Specimens must arrive at Integrated Oncology within 48 hours of collection.
Ship Immediately. Do not freeze. Ship with a cool pak.
Storage Instructions
Refrigerate (stable at room temperature up to 48 hours; refrigerated up to 48 hours).
Causes for Rejection
Samples with less than 1,000 WBC/ul may only have RBC analysis reported; specimens received for testing greater than 48 hours old and viability less than 80%; frozen specimen; broken container; fixed tissue; bone marrow; insufficient sample; sample greater than 48 hours old.
Test Details
Use
The following markers are utilized to test neutrophils and/or monocytes: CD24, CD15, CD45, FLAER, CD64, CD14. If a PNH clone is identified, the following markers are used to test red blood cells: glycophorin A, CD59.
Limitations
This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Methodology
Flow cytometry
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 502251 | Flow Cytometry PNH | 502253 | Interpretation: | 33664-4 | ||
| 502251 | Flow Cytometry PNH | 502254 | Comment: | N/A | ||
| 502251 | Flow Cytometry PNH | 502255 | Specimen: | 31208-2 | ||
| 502251 | Flow Cytometry PNH | 502256 | Submitted Dx: | N/A | ||
| 502251 | Flow Cytometry PNH | 502257 | Viability: | 33194-2 | ||
| 502251 | Flow Cytometry PNH | 502259 | Cell Population | N/A | ||
| 502251 | Flow Cytometry PNH | 502260 | Granulocytes: | 53831-4 | ||
| 502251 | Flow Cytometry PNH | 502262 | Monocytes: | 60554-3 | ||
| 502251 | Flow Cytometry PNH | 502261 | Red Blood Cells: | 49031-8 | ||
| 502251 | Flow Cytometry PNH | 502263 | Representative Histograms: | N/A | ||
| 502251 | Flow Cytometry PNH | 502264 | Antibodies Performed: | N/A | ||
| 502251 | Flow Cytometry PNH | 502274 | Comment: | N/A | ||
| 502251 | Flow Cytometry PNH | 503332 | Director Review | 72486-4 | ||
| 502251 | Flow Cytometry PNH | 481841 | 80563-0 |
© 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.
CPT Statement/Profile Statement
The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf