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17-Hydroxypregnenolone, Pre- and Post-ACTH Stimulation

CPT 84143(x2)
Synonyms
  • 17-Hydroxypregnenolone (Two Specimens)
  • Pregnenolone, 17-Hydroxy (Two Specimens)

Test Details

Methodology

High-pressure liquid chromatography (HPLC) tandem mass spectrometry

Result Turnaround Time

7 - 12 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

Adrenal Steroid Response to ACTH: Pediatrics

Use

This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient involved. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.

Special Instructions

Draw blood for baseline 17-OH pregnenolone. Inject cosyntropin 250 μg IM or IV (If IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.

Specimen Requirements

Specimen

Serum (preferred) or plasma, frozen

Volume

1 mL (each specimen)

Minimum Volume

0.4 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube

Collection Instructions

Transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Stability Requirements

Temperature

Period

Refrigerated

1 day

Frozen

2 years

Storage Instructions

Freeze

Causes for Rejection

Non-EDTA/heparin plasma; recently administered isotopes

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
500770 17-OH Pregnenolone, ACTH Stim. 500771 17-OH Pregnenolone Baseline ng/dL 6765-2
500770 17-OH Pregnenolone, ACTH Stim. 500772 17-OH Pregnenolone Stimulated ng/dL 13604-4
Order Code500770
Order Code Name17-OH Pregnenolone, ACTH Stim.
Order Loinc
Result Code500771
Result Code Name17-OH Pregnenolone Baseline
UofMng/dL
Result LOINC6765-2
Order Code500770
Order Code Name17-OH Pregnenolone, ACTH Stim.
Order Loinc
Result Code500772
Result Code Name17-OH Pregnenolone Stimulated
UofMng/dL
Result LOINC13604-4