Test Details
Methodology
High-pressure liquid chromatography (HPLC) tandem mass spectrometry
Result Turnaround Time
7 - 12 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient involved. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.
Special Instructions
Draw blood for baseline 17-OH pregnenolone. Inject cosyntropin 250 μg IM or IV (If IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.
Specimen Requirements
Specimen
Serum (preferred) or plasma, frozen
Volume
1 mL (each specimen)
Minimum Volume
0.4 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube
Collection Instructions
Transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Stability Requirements
| Temperature | Period |
|---|---|
| Refrigerated | 1 day |
| Frozen | 2 years |
Storage Instructions
Freeze
Causes for Rejection
Non-EDTA/heparin plasma; recently administered isotopes
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 500770 | 17-OH Pregnenolone, ACTH Stim. | 500771 | 17-OH Pregnenolone Baseline | ng/dL | 6765-2 | |
| 500770 | 17-OH Pregnenolone, ACTH Stim. | 500772 | 17-OH Pregnenolone Stimulated | ng/dL | 13604-4 | |
| Order Code | 500770 | |||||
| Order Code Name | 17-OH Pregnenolone, ACTH Stim. | |||||
| Order Loinc | ||||||
| Result Code | 500771 | |||||
| Result Code Name | 17-OH Pregnenolone Baseline | |||||
| UofM | ng/dL | |||||
| Result LOINC | 6765-2 | |||||
| Order Code | 500770 | |||||
| Order Code Name | 17-OH Pregnenolone, ACTH Stim. | |||||
| Order Loinc | ||||||
| Result Code | 500772 | |||||
| Result Code Name | 17-OH Pregnenolone Stimulated | |||||
| UofM | ng/dL | |||||
| Result LOINC | 13604-4 |