Test Details
Methodology
Liquid chromatography/tandem mass spectrometry (LC/MS-MS) after chromatography and solvent extraction
Result Turnaround Time
6 - 15 days
Use
Evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve. 11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol.
Special Instructions
For information on metyrapone testing, consult the Metyrapone Stimulation section of the Endocrine Appendix.
Specimen Requirements
Custom Additional Information
A primary use of 11-desoxycortisol testing is to detect congenital adrenal hyperplasia due to steroid 11-β-hydroxylase deficiency. This is a genetic disease caused by a deficiency of the CYP11B1 gene. 11-β-hydroxylase deficiency is transmitted as an autosomal recessive trait. It is associated with low-renin hypertension, hypokalemia, excess androgens (especially androstenedione), and genital ambiguity in affected females. The differential biochemical diagnosis of congenital adrenal hyperplasia caused by 11-β-hydroxylase deficiency includes finding increased serum concentrations of deoxycorticosterone, 11-desoxycortisol and androstenedione, and suppressed plasma renin concentrations. The disorder may be treated by administration of glucocorticoids. An additional use is to evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve. 11-deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol.
Specimen
Serum (preferred) or plasma
Volume
1 mL
Minimum Volume
0.2 mL
Container
Gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube
Storage Instructions
Freeze.
Collection Instructions
Serum/plasma must be separated from cells within 45 minutes of venipuncture. Send serum/plasma in a plastic transport tube.
Stability Requirements
Temperature | Period |
---|---|
Room temperature | 1 day |
Refrigerated | 1 day |
Frozen | 2 years |
Freeze/thaw cycles | Stable x3 |
Reference Range
See table.
Premature Infants | |
---|---|
26 to 28 wk, day 4 | 110−1376 ng/dL |
31 to 35 wk, day 4 | 48−579 ng/dL |
Full-term Infants | |
3 d | 13−147 ng/dL |
31 d to 11 m | <10−156 ng/dL |
Prepubertal Children | |
8 AM | 20−155 ng/dL |
Pubertal Children and Adults | |
8 AM | 12−158 ng/dL |
LOINC® Map
Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
---|---|---|---|---|---|---|
500171 | 11-Desoxycortisol | 1657-6 | 504584 | 11-Desoxycortisol | ng/dL | 1657-6 |
Order Code | 500171 | |||||
Order Code Name | 11-Desoxycortisol | |||||
Order Loinc | 1657-6 | |||||
Result Code | 504584 | |||||
Result Code Name | 11-Desoxycortisol | |||||
UofM | ng/dL | |||||
Result LOINC | 1657-6 |