Test Details
Methodology
Enzyme-linked immunosorbent assay (ELISA)
Result Turnaround Time
4 - 8 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Measure the quantity of PAI-1 in plasma, which may be useful in the determination of PAI-1 deficiency.6
Limitations
Samples containing platelets prior to freezing will give erroneously high results due to the release of latent PAI-1 from platelet granules.
Custom Additional Information
As PAI-1 is an acute phase reactant and also demonstrates diurnal variation, interpretation of results is difficult. Levels are highest in the morning. Deep vein thrombosis (DVT) and coronary artery disease (CAD) are associated with increased PAI-1 levels. Elevations have also been reported with diabetes mellitus, hypertension, obesity, insulin resistance, pregnancy, and with certain malignancies. Deficiency of PAI-1, an extremely rare and difficult diagnosis to make, has been associated with a bleeding diathesis. The PAI-1 antigen levels rise three- to sixfold during the third trimester of normal pregnancy. This is more pronounced in preëclamptic pregnancies. PAI-1 is the primary inhibitor of tissue plasminogen activator (tPA), a key enzyme in fibrinolysis. An increased plasma level of PAI-1 is associated with impaired fibrinolytic function. PAI-1 is found in several forms: active, inactive (latent), and complexed to tPA and urokinase plasminogen activator (uPA), all of which are measured in the PAI-1 antigen assay.
Specimen Requirements
Specimen
Plasma, frozen
Volume
0.5 mL
Container
Blue-top (sodium citrate) tube
Collection Instructions
Citrated plasma samples should be collected by double centrifugation. Platelet-poor plasma is needed. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.1 Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.2,3 The sample should be mixed immediately by gentle inversion at least six times to ensure adequate mixing of the anticoagulant with the blood. A discard tube is not required prior to collection of coagulation samples.4,5 When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tube. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and carefully remove 2/3 of the plasma using a plastic transfer pipette, being careful not to disturb the cells. Deliver to a plastic transport tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a Labcorp PP transpak frozen purple tube with screw cap (Labcorp No. 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
Please print and use the Volume Guide for Coagulation Testing to ensure proper draw volume.
Storage Instructions
Freeze.
Patient Preparation
The patient should be in a resting state and the specimen collected in the morning to avoid diurnal variation of results.
Causes for Rejection
Hemolysis; clotted specimen; specimen contaminated with heparin (ie, drawn with blood gases); specimens containing fibrin clots; specimen thawed
Footnotes
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 500057 | PAI-1 Antigen | 500059 | PAI-1 Antigen | ng/mL | 22758-7 | |
| Order Code | 500057 | |||||
| Order Code Name | PAI-1 Antigen | |||||
| Order Loinc | ||||||
| Result Code | 500059 | |||||
| Result Code Name | PAI-1 Antigen | |||||
| UofM | ng/mL | |||||
| Result LOINC | 22758-7 |