Test Details
Methodology
Immunohistochemistry (IHC). This is a semiquantitative measurement of ER/PR positive tumor nuclei in stained histologic tissue sections.
Result Turnaround Time
5 - 9 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Estrogen receptor analysis; progesterone receptor analysis
Use
Cancer prognosis: ER and PR positivity is a favorable prognostic factor in breast and endometrial carcinoma and meningioma.
Response to endocrine therapy: Combined ER and PR positivity is associated with increased response to antiestrogen therapies.
Special Instructions
Please direct any questions regarding this test to oncology customer service at 800-345-4363.
Limitations
In the absence of internal positive control staining, a false-negative result cannot be excluded. The presence or absence of positive internal control staining is indicated on all negative reports. Use of fixatives other than 10% formalin may not yield equivalent or satisfactory results.
Custom Additional Information
Estrogen receptors (ER) are cellular proteins that bind estrogens with a high affinity and specificity. They are a necessary component for estrogen-mediated cellular activity. The presence of progesterone receptors (PR) demonstrates an active ER mechanism for the induction of PR expression. Immunohistochemical staining permits the detection and localization of ER/PR within sections from formalin-fixed, paraffin-embedded tissue.
Clinical utilities for the measurement of ER/PR include patient prognosis and patient response to adjuvant endocrine therapy. Individuals with receptor-positive tumors generally have a better prognosis, as indicated by a longer interval to disease recurrence and a longer overall survival, than patients with receptor-negative tumors. Studies have also shown that the determination of receptor status by immunohistochemistry may be useful in predicting patient response to hormonal therapy.
Specimen Requirements
Specimen
Formalin-fixed, paraffin-embedded (FFPE) tumor
Volume
One paraffin block or seven unstained, positively-charged tissue slides cut at 4 to 5 microns and dried for one hour at 60°C
Container
Paraffin block transport pouch or slide mailer
Collection Instructions
Specimen should be fixed in 10% neutral-buffered formalin within one hour of collection. Optimum fixation time is six to 72 hours.
Storage Instructions
Maintain specimen at room temperature.
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 480277 | ER/PR,Immunohistochem,Paraffin | 480278 | Estrogen Receptor IHC | % | 14228-1 | |
| 480277 | ER/PR,Immunohistochem,Paraffin | 480279 | Progesterone Rec. IHC | % | 14230-7 | |
| 480277 | ER/PR,Immunohistochem,Paraffin | 480276 | Comment | 8251-1 | ||
| 480277 | ER/PR,Immunohistochem,Paraffin | 480268 | Fixative | N/A | ||
| 480277 | ER/PR,Immunohistochem,Paraffin | 480299 | Fixation Time | N/A | ||
| 480277 | ER/PR,Immunohistochem,Paraffin | 480298 | Additional Information | 19147-8 | ||
| Order Code | 480277 | |||||
| Order Code Name | ER/PR,Immunohistochem,Paraffin | |||||
| Order Loinc | ||||||
| Result Code | 480278 | |||||
| Result Code Name | Estrogen Receptor IHC | |||||
| UofM | % | |||||
| Result LOINC | 14228-1 | |||||
| Order Code | 480277 | |||||
| Order Code Name | ER/PR,Immunohistochem,Paraffin | |||||
| Order Loinc | ||||||
| Result Code | 480279 | |||||
| Result Code Name | Progesterone Rec. IHC | |||||
| UofM | % | |||||
| Result LOINC | 14230-7 | |||||
| Order Code | 480277 | |||||
| Order Code Name | ER/PR,Immunohistochem,Paraffin | |||||
| Order Loinc | ||||||
| Result Code | 480276 | |||||
| Result Code Name | Comment | |||||
| UofM | ||||||
| Result LOINC | 8251-1 | |||||
| Order Code | 480277 | |||||
| Order Code Name | ER/PR,Immunohistochem,Paraffin | |||||
| Order Loinc | ||||||
| Result Code | 480268 | |||||
| Result Code Name | Fixative | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 480277 | |||||
| Order Code Name | ER/PR,Immunohistochem,Paraffin | |||||
| Order Loinc | ||||||
| Result Code | 480299 | |||||
| Result Code Name | Fixation Time | |||||
| UofM | ||||||
| Result LOINC | N/A | |||||
| Order Code | 480277 | |||||
| Order Code Name | ER/PR,Immunohistochem,Paraffin | |||||
| Order Loinc | ||||||
| Result Code | 480298 | |||||
| Result Code Name | Additional Information | |||||
| UofM | ||||||
| Result LOINC | 19147-8 |