ANA by IFA, Reflex to 11-biomarker Profile, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B, Sm/RNP, Ribosomal P by Multiplex Immunoassay

Test Number 382965

Test number copied

CPT 86038

Synonyms

ANA by IFA Reflex

Test Details

Methodology

Indirect Immunofluorescence Assay (IFA) and Multiplex Flow Immunoassay

Result Turnaround Time

2 - 4 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Use

Assay for screening for ANAs in patients suspected of having connective tissue disease. The indirect immunofluorescent test has three elements to consider in the result:

1. Positive or negative fluorescence. A negative test is strong evidence against a diagnosis of SLE but not conclusive.

2. The titer (dilution) to which fluorescence remains positive (provides a reflection of the concentration or avidity of the antibody). Many individuals, particularly the elderly, may have low titer ANA without significant disease substantiated after work-up.

3. The pattern of nuclear fluorescence (reflecting specificity for various diseases).

Cytoplasmic (non-nuclear) staining patterns may also be noted with the IFA methodology.

Multiplex ANA detects up to 11 specific antibodies of the 100+ antibodies that may be found in the ANA IFA.

Special Instructions

If reflex test is performed, additional charges/CPT code(s) may apply.

Limitations

Males and females older than 80 years of age have a 50% incidence of low titer ANA. Various medications can induce a "lupoid" condition and elevated ANA titers. Usually the titer decreases following removal of the drug. Low antibody levels may be found in diseases other than connective tissue disease.

Specimen Requirements

Specimen

Serum

Volume

1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)

Container

Gel-barrier tube

Stability Requirements

Temperature	Period
Room temperature	7 days
Refrigerated	7 days
Frozen	7 days
Freeze/thaw cycles	Stable x2

Storage Instructions

Room temperature

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination

LOINC® Map

Order Code	Order Code Name	Result Code	Result Code Name	Result LOINC
382965	ANA, IFA Rfx 11 Mark Multiplex	164947	ANA by IFA Rfx Titer/Pattern	42254-3
Order Code	382965
Order Code Name	ANA, IFA Rfx 11 Mark Multiplex
Order Loinc
Result Code	164947
Result Code Name	ANA by IFA Rfx Titer/Pattern
UofM
Result LOINC	42254-3

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016279	Homogeneous Pattern	20398-4
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016279
Result Name	Homogeneous Pattern
UofM
Result LOINC	20398-4

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016287	Nucleolar Pattern	20399-2
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016287
Result Name	Nucleolar Pattern
UofM
Result LOINC	20399-2

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016295	Speckled Pattern	20401-6
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016295
Result Name	Speckled Pattern
UofM
Result LOINC	20401-6

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016303	Centromere Pattern	5077-3
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016303
Result Name	Centromere Pattern
UofM
Result LOINC	5077-3

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016264	Spindle Apparatus Pattern	53003-0
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016264
Result Name	Spindle Apparatus Pattern
UofM
Result LOINC	53003-0

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016265	Nuclear Membrane Pattern	54006-2
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016265
Result Name	Nuclear Membrane Pattern
UofM
Result LOINC	54006-2

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016266	Midbody Pattern	54155-7
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016266
Result Name	Midbody Pattern
UofM
Result LOINC	54155-7

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016267	Nuclear Dot Pattern	53998-1
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016267
Result Name	Nuclear Dot Pattern
UofM
Result LOINC	53998-1

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016268	PCNA Pattern	53006-3
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016268
Result Name	PCNA Pattern
UofM
Result LOINC	53006-3

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016269	Centriole Pattern	54151-6
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016269
Result Name	Centriole Pattern
UofM
Result LOINC	54151-6

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	012625	FANA Staining Patterns	016271	Note:	N/A
Reflex 1
Order Code	012625
Order Name	FANA Staining Patterns
Result Code	016271
Result Name	Note:
UofM
Result LOINC	N/A

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016279	Homogeneous Pattern	20398-4
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016279
Result Name	Homogeneous Pattern
UofM
Result LOINC	20398-4

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016287	Nucleolar Pattern	20399-2
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016287
Result Name	Nucleolar Pattern
UofM
Result LOINC	20399-2

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016295	Speckled Pattern	20401-6
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016295
Result Name	Speckled Pattern
UofM
Result LOINC	20401-6

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016303	Centromere Pattern	5077-3
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016303
Result Name	Centromere Pattern
UofM
Result LOINC	5077-3

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016264	Spindle Apparatus Pattern	53003-0
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016264
Result Name	Spindle Apparatus Pattern
UofM
Result LOINC	53003-0

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016265	Nuclear Membrane Pattern	54006-2
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016265
Result Name	Nuclear Membrane Pattern
UofM
Result LOINC	54006-2

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016266	Midbody Pattern	54155-7
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016266
Result Name	Midbody Pattern
UofM
Result LOINC	54155-7

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016267	Nuclear Dot Pattern	53998-1
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016267
Result Name	Nuclear Dot Pattern
UofM
Result LOINC	53998-1

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016268	PCNA Pattern	53006-3
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016268
Result Name	PCNA Pattern
UofM
Result LOINC	53006-3

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016269	Centriole Pattern	54151-6
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016269
Result Name	Centriole Pattern
UofM
Result LOINC	54151-6

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016271	Note:	N/A
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016271
Result Name	Note:
UofM
Result LOINC	N/A

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	096343	Anti-DNA (DS) Ab Qn	IU/mL	5130-0
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	096343
Result Name	Anti-DNA (DS) Ab Qn
UofM	IU/mL
Result LOINC	5130-0

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016354	RNP Antibodies	AI	29374-6
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016354
Result Name	RNP Antibodies
UofM	AI
Result LOINC	29374-6

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016362	Smith Antibodies	AI	11090-8
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016362
Result Name	Smith Antibodies
UofM	AI
Result LOINC	11090-8

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	016376	Smith/RNP Antibodies	AI	27048-8
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	016376
Result Name	Smith/RNP Antibodies
UofM	AI
Result LOINC	27048-8

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	018707	Antiscleroderma-70 Antibodies	AI	27416-7
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	018707
Result Name	Antiscleroderma-70 Antibodies
UofM	AI
Result LOINC	27416-7

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	012684	Sjogren's Anti-SS-A	AI	17792-3
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	012684
Result Name	Sjogren's Anti-SS-A
UofM	AI
Result LOINC	17792-3

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	012692	Sjogren's Anti-SS-B	AI	17791-5
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	012692
Result Name	Sjogren's Anti-SS-B
UofM	AI
Result LOINC	17791-5

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	012581	Antichromatin Antibodies	AI	51775-5
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	012581
Result Name	Antichromatin Antibodies
UofM	AI
Result LOINC	51775-5

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	012701	Antiribosomal P Antibodies	AI	13636-6
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	012701
Result Name	Antiribosomal P Antibodies
UofM	AI
Result LOINC	13636-6

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	161457	Anti-Jo-1	AI	11565-9
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	161457
Result Name	Anti-Jo-1
UofM	AI
Result LOINC	11565-9

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	UofM	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	164816	Anti-Centromere B Antibodies	AI	53982-5
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	164816
Result Name	Anti-Centromere B Antibodies
UofM	AI
Result LOINC	53982-5

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	164879	See below:	N/A
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	164879
Result Name	See below:
UofM
Result LOINC	N/A

Reflex Table for ANA by IFA Rfx Titer/Pattern
	Order Code	Order Name	Result Code	Result Name	Result LOINC
Reflex 1	160065	ENA+DNA/DS+Antich+Centro+FA...	164949	ANA IFA Tracking to BioPlex	N/A
Reflex 1
Order Code	160065
Order Name	ENA+DNA/DS+Antich+Centro+FA...
Result Code	164949
Result Name	ANA IFA Tracking to BioPlex
UofM
Result LOINC	N/A

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