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ANA by IFA, Reflex to 9-biomarker profile, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B by Multiplex Immunoassay

Test Number 340897
Test number copied
CPT 86038
Synonyms
  • ANA IFA Reflex

Test Details

Methodology

Indirect immunofluorescence assay (IFA) and multiplex flow immunoassay

Result Turnaround Time

2 - 4 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Information

Related Documents

For more information, please view the literature below.

Antinuclear Antibodies Initial Screening Profiles Flyer

Lupus Comprehensive Testing Brochure

Test Includes

Detect antibodies to nuclear antigens by IFA with patterns and titers with reflex if positive to centromere B; chromatin; dsDNA; Jo-1; RNP; scl-70; Sjögren's A; Sjögren's B; Smith (Sm). If reflex testing is performed, additional charges/CPT code(s) may apply.

Use

Assay for screening for ANAs in patients suspected of having connective tissue disease.

Limitations

Males and females older than 80 years of age have a 50% incidence of low titer ANA. Various medications can induce a "lupoid" condition and elevated ANA titers. Usually the titer decreases following removal of the drug. Low antibody levels may be found in diseases other than connective tissue disease.

Custom Additional Information

The indirect immunofluorescent test has three elements to consider in the result:

1. Positive or negative fluorescence. A negative test is strong evidence against a diagnosis of SLE but not conclusive.

2. The titer (dilution) to which fluorescence remains positive (provides a reflection of the concentration or avidity of the antibody). Many individuals, particularly the elderly, may have low titer ANA without significant disease substantiated after work-up.

3. The pattern of nuclear fluorescence (reflecting specificity for various diseases).

Cytoplasmic (non-nuclear) staining patterns may also be noted with the IFA methodology.

Multiplex ANA detects up to 11 specific antibodies of the 100+ antibodies that may be found in the ANA IFA.

Specimen Requirements

Specimen

Serum

Volume

1 mL

Minimum Volume

0.5 mL

Container

Red-top tube or gel-barrier tube

Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

7 days

Frozen

7 days

Freeze/thaw cycles

Stable x2

Storage Instructions

Room temperature

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
340897 ANA, IFA Rfx 9 Mark Multiplex 5048-4 164947 ANA by IFA Rfx Titer/Pattern 42254-3
340897 ANA, IFA Rfx 9 Mark Multiplex 5048-4 164945 Please Note: 77202-0
Order Code340897
Order Code NameANA, IFA Rfx 9 Mark Multiplex
Order Loinc5048-4
Result Code164947
Result Code NameANA by IFA Rfx Titer/Pattern
UofM
Result LOINC42254-3
Order Code340897
Order Code NameANA, IFA Rfx 9 Mark Multiplex
Order Loinc5048-4
Result Code164945
Result Code NamePlease Note:
UofM
Result LOINC77202-0
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016279 Homogeneous Pattern 20398-4
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016279
Result NameHomogeneous Pattern
UofM
Result LOINC20398-4
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016287 Nucleolar Pattern 20399-2
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016287
Result NameNucleolar Pattern
UofM
Result LOINC20399-2
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016295 Speckled Pattern 20401-6
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016295
Result NameSpeckled Pattern
UofM
Result LOINC20401-6
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016303 Centromere Pattern 5077-3
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016303
Result NameCentromere Pattern
UofM
Result LOINC5077-3
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016264 Spindle Apparatus Pattern 53003-0
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016264
Result NameSpindle Apparatus Pattern
UofM
Result LOINC53003-0
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016265 Nuclear Membrane Pattern 54006-2
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016265
Result NameNuclear Membrane Pattern
UofM
Result LOINC54006-2
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016266 Midbody Pattern 54155-7
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016266
Result NameMidbody Pattern
UofM
Result LOINC54155-7
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016267 Nuclear Dot Pattern 53998-1
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016267
Result NameNuclear Dot Pattern
UofM
Result LOINC53998-1
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016268 PCNA Pattern 53006-3
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016268
Result NamePCNA Pattern
UofM
Result LOINC53006-3
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016269 Centriole Pattern 54151-6
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016269
Result NameCentriole Pattern
UofM
Result LOINC54151-6
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016271 Note: N/A
Reflex 1
Order Code012625
Order NameFANA Staining Patterns
Result Code016271
Result NameNote:
UofM
Result LOINCN/A
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016279 Homogeneous Pattern 20398-4
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016279
Result NameHomogeneous Pattern
UofM
Result LOINC20398-4
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016287 Nucleolar Pattern 20399-2
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016287
Result NameNucleolar Pattern
UofM
Result LOINC20399-2
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016295 Speckled Pattern 20401-6
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016295
Result NameSpeckled Pattern
UofM
Result LOINC20401-6
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016303 Centromere Pattern 5077-3
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016303
Result NameCentromere Pattern
UofM
Result LOINC5077-3
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016264 Spindle Apparatus Pattern 53003-0
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016264
Result NameSpindle Apparatus Pattern
UofM
Result LOINC53003-0
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016265 Nuclear Membrane Pattern 54006-2
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016265
Result NameNuclear Membrane Pattern
UofM
Result LOINC54006-2
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016266 Midbody Pattern 54155-7
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016266
Result NameMidbody Pattern
UofM
Result LOINC54155-7
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016267 Nuclear Dot Pattern 53998-1
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016267
Result NameNuclear Dot Pattern
UofM
Result LOINC53998-1
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016268 PCNA Pattern 53006-3
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016268
Result NamePCNA Pattern
UofM
Result LOINC53006-3
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016269 Centriole Pattern 54151-6
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016269
Result NameCentriole Pattern
UofM
Result LOINC54151-6
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016271 Note: N/A
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016271
Result NameNote:
UofM
Result LOINCN/A
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 096343 Anti-DNA (DS) Ab Qn IU/mL 5130-0
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code096343
Result NameAnti-DNA (DS) Ab Qn
UofMIU/mL
Result LOINC5130-0
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016354 RNP Antibodies AI 29374-6
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016354
Result NameRNP Antibodies
UofMAI
Result LOINC29374-6
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016362 Smith Antibodies AI 11090-8
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code016362
Result NameSmith Antibodies
UofMAI
Result LOINC11090-8
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 018707 Antiscleroderma-70 Antibodies AI 27416-7
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code018707
Result NameAntiscleroderma-70 Antibodies
UofMAI
Result LOINC27416-7
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 012684 Sjogren's Anti-SS-A AI 17792-3
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code012684
Result NameSjogren's Anti-SS-A
UofMAI
Result LOINC17792-3
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 012692 Sjogren's Anti-SS-B AI 17791-5
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code012692
Result NameSjogren's Anti-SS-B
UofMAI
Result LOINC17791-5
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 012581 Antichromatin Antibodies AI 51775-5
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code012581
Result NameAntichromatin Antibodies
UofMAI
Result LOINC51775-5
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 161457 Anti-Jo-1 AI 11565-9
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code161457
Result NameAnti-Jo-1
UofMAI
Result LOINC11565-9
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 164816 Anti-Centromere B Antibodies AI 53982-5
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code164816
Result NameAnti-Centromere B Antibodies
UofMAI
Result LOINC53982-5
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 164869 See below: N/A
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code164869
Result NameSee below:
UofM
Result LOINCN/A
Reflex Table for ANA by IFA Rfx Titer/Pattern
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 164949 ANA IFA Tracking to BioPlex N/A
Reflex 1
Order Code160024
Order NameENA+DNA/DS+Antich+Centro+FA...
Result Code164949
Result NameANA IFA Tracking to BioPlex
UofM
Result LOINCN/A