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For hours, walk-ins and appointments.Protein Electrophoresis With Interpretation, 24-Hour Urine
CPT:
84156; 84166
Synonyms
- Interpretation, Protein Electrophoresis, Urine
Test Includes
Evaluation of protein electrophoretic bands; protein electrophoresis, urine; quantitation of M-spike if present
Special Instructions
State 24-hour volume on the request form.
Expected Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
Specimen Requirements
Specimen
Urine (24-hour)
Volume
20-mL aliquot
Minimum Volume
6-mL aliquot
Container
12-mL plastic transport tube (Labcorp No. 23597) without preservative
Collection
Mix well.
Storage Instructions
Refrigerated (preferred) or room temperature
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x1 |
Causes for Rejection
Preservative added
Test Details
Use
Work up myeloma, macroglobulinemia of Waldenström, lymphoma, amyloidosis; differentiate between normal renal function, glomerular proteinuria, and tubular proteinuria. Increased glomerular permeability leads to higher concentrations of large proteins in the glomerular filtrate. Diminished tubular reabsorptive capacity results in a marked increase in urinary excretion of low molecular weight problems.
Methodology
Electrophoresis on agarose media
Reference Interval
Normal pattern. See table.
Pattern Description | Electrophoretic Zones | |||||
|---|---|---|---|---|---|---|
Total Protein (mg/d) | Albumin | α1 | α2 | β | γ | |
*Looks like serum protein electrophoretogram. | ||||||
+ = mildly elevated. | ||||||
++ = moderately elevated. | ||||||
+++ = markedly elevated. | ||||||
+/– = may or may not be present. | ||||||
Normal | <150 | +/– | +/– | +/– | ||
Selective glomerular proteinuria | ||||||
mild | <1500 | ++ | + | + | ||
severe | >1500 | +++ | ++ | ++ | ||
Nonselective glomerular proteinuria* | >3000 | +++ | ++ | ++ | ++ | ++ |
Tubular proteinuria | <1500 | Trace to + | Trace | ++ | ++ | Trace of light chain |
Mixed glomerular and tubular proteinuria | +++ | + | ++ | + | + | |
Albuminuria | >150 | ++ | ||||
Overflow proteinuria from acute phase response | Trace or present | ++ | ++ | Trace | Trace | |
Overflow proteinuria with monoclonal spike | (spike) or ++ | (spike) or ++ | ||||
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 261511 | Prot Electro+Interp, 24-Hr Ur | 013664 | Protein,Total,Urine | mg/dL | 2888-6 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 013219 | Prot,24hr calculated | mg/24 hr | 2889-4 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133686 | Albumin, U | % | 13986-5 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133694 | Alpha-1-Globulin, U | % | 13984-0 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133702 | Alpha-2-Globulin, U | % | 13987-3 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133710 | Beta Globulin, U | % | 13988-1 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133728 | Gamma Globulin, U | % | 13989-9 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133729 | M-Spike, % | % | 42484-6 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133734 | M-Spike, mg/24 hr | mg/24 hr | 42482-0 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133800 | Please note: | 77202-0 | ||
| 261511 | Prot Electro+Interp, 24-Hr Ur | 003724 | 11502-2 | |||
| 261511 | Prot Electro+Interp, 24-Hr Ur | 120030 | P E Interpretation, U | 49301-5 |
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