Test Details
Methodology
Electrophoresis on agarose media
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Evaluation of protein electrophoretic bands; protein electrophoresis, urine; quantitation of M-spike if present
Use
Work up myeloma, macroglobulinemia of Waldenström, lymphoma, amyloidosis; differentiate between normal renal function, glomerular proteinuria, and tubular proteinuria. Increased glomerular permeability leads to higher concentrations of large proteins in the glomerular filtrate. Diminished tubular reabsorptive capacity results in a marked increase in urinary excretion of low molecular weight problems.
Special Instructions
State 24-hour volume on the request form.
Specimen Requirements
Specimen
Urine (24-hour)
Volume
20-mL aliquot
Minimum Volume
6-mL aliquot
Container
12-mL plastic transport tube (Labcorp No. 23597) without preservative
Collection Instructions
Mix well.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x1 |
Reference Range
Normal pattern. See table.
Pattern Description | Electrophoretic Zones | |||||
|---|---|---|---|---|---|---|
Total Protein (mg/d) | Albumin | α1 | α2 | β | γ | |
*Looks like serum protein electrophoretogram. | ||||||
+ = mildly elevated. | ||||||
++ = moderately elevated. | ||||||
+++ = markedly elevated. | ||||||
+/– = may or may not be present. | ||||||
Normal | <150 | +/– | +/– | +/– | ||
Selective glomerular proteinuria | ||||||
mild | <1500 | ++ | + | + | ||
severe | >1500 | +++ | ++ | ++ | ||
Nonselective glomerular proteinuria* | >3000 | +++ | ++ | ++ | ++ | ++ |
Tubular proteinuria | <1500 | Trace to + | Trace | ++ | ++ | Trace of light chain |
Mixed glomerular and tubular proteinuria | +++ | + | ++ | + | + | |
Albuminuria | >150 | ++ | ||||
Overflow proteinuria from acute phase response | Trace or present | ++ | ++ | Trace | Trace | |
Overflow proteinuria with monoclonal spike | (spike) or ++ | (spike) or ++ | ||||
Storage Instructions
Refrigerated (preferred) or room temperature
Causes for Rejection
Preservative added
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 261511 | Prot Electro+Interp, 24-Hr Ur | 013664 | Protein,Total,Urine | mg/dL | 2888-6 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 013219 | Prot,24hr calculated | mg/24 hr | 2889-4 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133686 | Albumin, U | % | 13986-5 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133694 | Alpha-1-Globulin, U | % | 13984-0 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133702 | Alpha-2-Globulin, U | % | 13987-3 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133710 | Beta Globulin, U | % | 13988-1 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133728 | Gamma Globulin, U | % | 13989-9 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133729 | M-Spike, % | % | 42484-6 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133734 | M-Spike, mg/24 hr | mg/24 hr | 42482-0 | |
| 261511 | Prot Electro+Interp, 24-Hr Ur | 133800 | Please note: | 77202-0 | ||
| 261511 | Prot Electro+Interp, 24-Hr Ur | 003724 | 11502-2 | |||
| 261511 | Prot Electro+Interp, 24-Hr Ur | 120030 | P E Interpretation, U | 49301-5 | ||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 013664 | |||||
| Result Code Name | Protein,Total,Urine | |||||
| UofM | mg/dL | |||||
| Result LOINC | 2888-6 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 013219 | |||||
| Result Code Name | Prot,24hr calculated | |||||
| UofM | mg/24 hr | |||||
| Result LOINC | 2889-4 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133686 | |||||
| Result Code Name | Albumin, U | |||||
| UofM | % | |||||
| Result LOINC | 13986-5 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133694 | |||||
| Result Code Name | Alpha-1-Globulin, U | |||||
| UofM | % | |||||
| Result LOINC | 13984-0 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133702 | |||||
| Result Code Name | Alpha-2-Globulin, U | |||||
| UofM | % | |||||
| Result LOINC | 13987-3 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133710 | |||||
| Result Code Name | Beta Globulin, U | |||||
| UofM | % | |||||
| Result LOINC | 13988-1 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133728 | |||||
| Result Code Name | Gamma Globulin, U | |||||
| UofM | % | |||||
| Result LOINC | 13989-9 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133729 | |||||
| Result Code Name | M-Spike, % | |||||
| UofM | % | |||||
| Result LOINC | 42484-6 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133734 | |||||
| Result Code Name | M-Spike, mg/24 hr | |||||
| UofM | mg/24 hr | |||||
| Result LOINC | 42482-0 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 133800 | |||||
| Result Code Name | Please note: | |||||
| UofM | ||||||
| Result LOINC | 77202-0 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 003724 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 11502-2 | |||||
| Order Code | 261511 | |||||
| Order Code Name | Prot Electro+Interp, 24-Hr Ur | |||||
| Order Loinc | ||||||
| Result Code | 120030 | |||||
| Result Code Name | P E Interpretation, U | |||||
| UofM | ||||||
| Result LOINC | 49301-5 |