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Epstein-Barr Virus (EBV) Antibody Profile

CPT 86664; 86665(x2)
Synonyms
  • EBV
  • Mononucleosis

Test Details

Methodology

Chemiluminescent Immunoassay (CLIA)

Result Turnaround Time

1 - 2 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

EBV-VCA, IgG; EBV-VCA, IgM; Epstein-Barr nuclear antigen antibodies (EBNA); interpretation

Use

Aid in determining past or present infection with EBV as well as susceptibility to future infection.

Limitations

The results from this panel are not by themselves diagnostic and should be considered in association with other clinical data and patient symptoms. This test is intended for qualitative determination only. The numeric value of the final result above the cutoff is not indicative of the amount of antibodies present.

Custom Additional Information

Epstein-Barr (EB) virus is a herpes group virus that is ubiquitous. It is the cause of classic infectious mononucleosis and is causally implicated in the pathogenesis of Burkitt lymphoma, some nasopharyngeal carcinomas, and rare hereditary lymphoproliferative disorders. The serologic response to EB virus includes antibody to early antigen, IgM and IgG antibodies to viral capsid antigen (VCA), and antibodies to Epstein-Barr nuclear antigen (EBNA).

Although most cases of infectious mononucleosis can be diagnosed on the basis of clinical findings, blood count and morphology, and a positive test for heterophile antibody, as many as 20% may be heterophile-negative, at least at presentation (Heterophile may become positive when repeated in a few days). In some of these cases, a test for Epstein-Barr virus antibodies may be useful.

The most controversial use of EBV serology is in chronic fatigue syndrome, a complaint predominantly (but not exclusively) of young to middle-aged women, characterized by long persistent debilitating fatigue and a panoply of usually mild somatic complaints. The high levels of EBV antibodies in the general population, their long persistence, and the poor correlation of antibody titers with symptoms combine to make EBV serology useless in diagnosing, following, or ruling out chronic fatigue syndrome. See table.

EBV Interpretation Table

Interpretation

VCA-IgM

VCA-IgG

EBNA-IgG

Key — Antibody present: (+); Antibody absent: (−)

No previous infection/Susceptibility

Primary infection (new or recent)

+

+

Past infection

±

+

+

See comment below*

+

*Results indicate infection with EBV at some time however cannot predict the timing of the infection since antibodies to EBNA usually develop after primary infection or, alternatively, approximately 5-10% of patients with EBV never develop antibodies to EBNA.

Specimen Requirements

Specimen

Serum

Volume

2 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x4

Storage Instructions

Room temperature

Causes for Rejection

Hemolysis; lipemia; grossly icteric; visible particulate matter; gross bacterial contamination

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
240610 EBV Antibody Profile 87554-2 096739 EBV Ab VCA, IgM U/mL 5159-9
240610 EBV Antibody Profile 87554-2 096235 EBV Ab VCA, IgG U/mL 5157-3
240610 EBV Antibody Profile 87554-2 010276 EBV Nuclear Antigen Ab, IgG U/mL 30083-0
240610 EBV Antibody Profile 87554-2 169993 Interpretation: 8251-1
Order Code240610
Order Code NameEBV Antibody Profile
Order Loinc87554-2
Result Code096739
Result Code NameEBV Ab VCA, IgM
UofMU/mL
Result LOINC5159-9
Order Code240610
Order Code NameEBV Antibody Profile
Order Loinc87554-2
Result Code096235
Result Code NameEBV Ab VCA, IgG
UofMU/mL
Result LOINC5157-3
Order Code240610
Order Code NameEBV Antibody Profile
Order Loinc87554-2
Result Code010276
Result Code NameEBV Nuclear Antigen Ab, IgG
UofMU/mL
Result LOINC30083-0
Order Code240610
Order Code NameEBV Antibody Profile
Order Loinc87554-2
Result Code169993
Result Code NameInterpretation:
UofM
Result LOINC8251-1