Herpes Simplex Virus (HSV) Types 1 and 2, NAA

CPT: 87529(x2)
Print Share

Expected Turnaround Time

2 - 4 days



Specimen Requirements


Specimen

Vesicular fluid, ulcerated lesions, vaginal, or endocervical cells collected by one of the methods described below


Volume

One swab or entire liquid cytology vial


Minimum Volume

One swab or entire liquid cytology vial


Container

Aptima® unisex or Aptima® vaginal swab transport or swab in Universal or Viral Transport Media (UTM/VTM) or ThinPrep® liquid cytology vial


Collection

Lesion/vesicle swab: Unroof or scrape the lesion with an Aptima® swab (for Aptima® collections) or with a synthetic swab for UTM/VTM collections.

Vaginal swab: Collect vaginal fluid sample using the Aptima® vaginal swab kit by contacting the swab to the lower third of the vaginal wall and rotating the swab for 10 to 30 seconds to absorb fluid. Immediately place the swab into the transport tube and carefully break the swab shaft against the side of the tube. Tightly screw on the cap.

Endocervical swab in Aptima®: Remove excess mucus from the cervical os and surrounding mucosa using the cleaning swab (white-shafted swab in the package with red printing). Discard this swab. Insert the specimen collection swab (blue-shafted swab in the package with green printing) into the endocervical canal. Gently rotate the swab clockwise for 10 to 30 seconds in the endocervical canal to ensure adequate sampling. Withdraw the swab carefully; avoid contact with the vaginal mucosa. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Carefully break the swab shaft at the scoreline using care to avoid splashing of the contents. Recap the swab specimen transport tube tightly.

ThinPrep® Vial − Broom or Brush/Spatula

Broom-like collection technique: Obtain a sample from the cervix using a broom-like device by inserting the brush portion into the cervical os and then rotating the brush five times. Rinse the collection device in the PreservCyt® solution by pushing the brush into the bottom of the vial 10 times, forcing the bristles to bend apart to release the cervical material. As a final step, twirl the brush between the thumb and forefinger vigorously to release additional cellular material. Discard the collection device. Tighten the cap on the ThinPrep® vial so that the torque line on the cap passes the torque line on the vial.

Brush/spatula technique: Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Slowly rotate the brush ¼ to ½ turn in one direction. Do not over-rotate the brush. Then, rotate the brush in the PreservCyt® solution 10 times while pushing against the wall of the ThinPrep® vial. Swirl the brush vigorously to release additional material. Discard the brush. Obtain an adequate sample from the ectocervix using a plastic spatula. Swirl vigorously in the ThinPrep® vial 10 times and discard the spatula. Tighten the cap on the ThinPrep® container so that the torque line on the cap passes the torque line on the vial.


Storage Instructions

Maintain specimen at ambient or refrigerated temperature.


Causes for Rejection

Urine specimen; specimen transported under inappropriate conditions; bacterial swabs; unlabeled specimen or discrepancy between specimen label and test request form; Aptima® COMBO 2 (AC2) swab specimen transport tube with two swabs or swab not supplied by Hologic or no swab; ProbeTec™ swabs; female urethral swab; SurePath™ vial; Aptima® swab transports >7 days from collection; specimens submitted in non-FDA cleared UTM or VTM


Test Details


Use

This test is used to detect and type active HSV shedding.


Limitations

This test is intended for use as an aid in the diagnosis of herpes simplex virus (HSV) infections with active viral shedding; it also differentiates HSV-1 from HSV-2. Negative HSV NAA results indicate lack of viral shedding but do not confirm absence of previous infection.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.


Methodology

Nucleic acid amplification (NAA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
188056 HSV NAA 188057 HSV 1 NAA 16130-7
188056 HSV NAA 188058 HSV 2 NAA 16131-5

For Providers

Please login to order a test

Order a Test

© 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf