Test Details
Methodology
Chromogenic enzyme activity test
Result Turnaround Time
2 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Enzyme activity test for use in the detection of vaginal fluid specimens for sialidase activity, an enzyme produced by bacteria associated with bacterial vaginitis, including Bacteroides spp, Prevotella spp, and Mobiluncus spp and some G vaginalis. In a recent study, Bradshaw et al demonstrated the test to be 88% sensitive and 95% specific.
Limitations
Collecting specimens from the cervix should be avoided because (a) it might increase risk to OB patients, and (b) cervical sialidase activity is usually higher than vaginal sialidase activity. Do not collect specimens from patients who have (a) used a vaginal cream or ointment product, (b) douched, or (c) used spermicides, vaginal lubricants, or feminine sprays within 72 hours of testing. If insufficient sample is collected or collected from a patient undergoing antimicrobial therapy, the test may give a false-negative result.
References
Specimen Requirements
Specimen
Vaginal fluid
Volume
Swab
Container
Copan double swab in Amies liquid medium
Collection Instructions
Using transport swabs provided, obtain a sample by contacting the lower one-third of the vaginal wall, rotating 10 to 20 seconds to absorb fluid. Immediately place the swabs into the transport tube.
Storage Instructions
Maintain specimen at room temperature for up to 48 hours.
Causes for Rejection
Specimen collected or transported other than previously described
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 180034 | BV, Sialidase Activity | 41477-1 | 180035 | BV, Sialidase Activity | 41477-1 | |
| Order Code | 180034 | |||||
| Order Code Name | BV, Sialidase Activity | |||||
| Order Loinc | 41477-1 | |||||
| Result Code | 180035 | |||||
| Result Code Name | BV, Sialidase Activity | |||||
| UofM | ||||||
| Result LOINC | 41477-1 |