Test Details
Methodology
PHA stimulation ATP luciferase assay. ImmuKnow® is a trademark of Viracor-IBT Laboratories Inc, Lee's Summit, Mo.
Result Turnaround Time
2 - 4 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is a transplant physician's means of obtaining and monitoring information about immune status. ImmuKnow® measures the adenosine triphosphate (ATP) response of stimulated peripheral blood lymphocytes.
Special Instructions
Prior to initial submission of specimens for this test, contact HLA customer service (800-533-1037) to establish special specimen collection and transportation arrangements. These specimen collection and transportation arrangements must be observed in order to obtain acceptable results.
Specimen Requirements
Specimen
Whole blood (sodium heparin only)
Volume
6 to 8 mL
Minimum Volume
2 to 4 mL
Container
Green-top (sodium heparin) tube only
Collection Instructions
Do not use lithium heparin, ACD, or EDTA tubes. Assay must be set up within 30 hours of specimen collection. Specimens received after 30 hours are not acceptable for testing.
Storage Instructions
Maintain specimen at room temperature (ie, 18°C to 28°C) only. Specimen stable 30 hours; >30 hours specimen not viable. Do not refrigerate or freeze specimen.
Causes for Rejection
Specimen chilled, refrigerated, or frozen; >30 hours since collection; clotted or hemolyzed specimen; lithium heparin used
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 176120 | ImmuKnow (R) | 53939-5 | 176101 | ImmuKnow (R) | ng/mL ATP | 53939-5 |
| Order Code | 176120 | |||||
| Order Code Name | ImmuKnow (R) | |||||
| Order Loinc | 53939-5 | |||||
| Result Code | 176101 | |||||
| Result Code Name | ImmuKnow (R) | |||||
| UofM | ng/mL ATP | |||||
| Result LOINC | 53939-5 |