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HLA Class I Antibody and HLA Class II Antibody Screen

CPT 86828
Synonyms
  • HLA Antibody, PRA
  • HLA Class 1/2 Antibody, White Cell Antibody

Test Details

Methodology

Solid phase (Luminex)

Result Turnaround Time

3 - 5 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Use

Solid organ transplantation

Special Instructions

Collect specimen prior to dialysis. Do not collect if patient has received antithymocyte globulin, OKT3, or alemtuzumab (Campath®) recently. These drugs may cause false-positive results.

Limitations

This test provides limited information as to the HLA specificities of the antibodies. Detects antibodies of the IgG isotype and not IgM antibodies, which are usually indicative of autoantibodies.

Specimen Requirements

Specimen

Serum

Volume

7 mL

Minimum Volume

3 mL

Container

Red-top tube or gel-barrier tube

Collection Instructions

To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Stability Requirements

Temperature

Period

Room temperature

2 days

Refrigerated

1 month

Frozen

2 years

Storage Instructions

Maintain specimen at room temperature. Protect from extreme heat or cold. Serum may be frozen.

Causes for Rejection

Highly lipemic or hemolyzed specimen

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
168050 HLA Class 1,2 Antibody Screen 167104 Class 1 Ab Screen 50968-7
168050 HLA Class 1,2 Antibody Screen 167105 Class II Ab Screen 50968-7
168050 HLA Class 1,2 Antibody Screen 167106 Comment: 49549-9
Order Code168050
Order Code NameHLA Class 1,2 Antibody Screen
Order Loinc
Result Code167104
Result Code NameClass 1 Ab Screen
UofM
Result LOINC50968-7
Order Code168050
Order Code NameHLA Class 1,2 Antibody Screen
Order Loinc
Result Code167105
Result Code NameClass II Ab Screen
UofM
Result LOINC50968-7
Order Code168050
Order Code NameHLA Class 1,2 Antibody Screen
Order Loinc
Result Code167106
Result Code NameComment:
UofM
Result LOINC49549-9