Test Details
Methodology
HLA results are obtained using Next Generation Sequencing (NGS). Supplemental procedures based on Sanger sequence based typing (SBT) may be used as needed to obtain the required resolution.
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HLA results are obtained using Next Generation Sequencing (NGS). Supplemental procedures based on Sanger sequence based typing (SBT) may be used as needed to obtain the required resolution. |
Result Turnaround Time
3 - 7 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Test provides data for clients to use for matching transplant donors and recipients. Specific HLA A alleles may be correlated with certain disease states or other clinical conditions. This test may be used to identify specific alleles (except for HLA A*02:01 [167107]).
CWD resolution typing for HLA will meet the following criteria:
1. The typing must contain only one unambiguously assigned genotype, or
2. The typing may contain multiple alternative genotypes if one includes two Common and Well Documented (CWD) alleles and the others do not include any CWD allele. Alleles that encode identical protein sequences in the antigen recognition site (ARS) will not be resolved even if they are CWD.
3. Rare or unusual allele combinations may not be ruled out.
4. Allele codes will be reported to include those alleles that are not ruled out.
Common and well documented alleles are defined by Cano et al1 and Mack et al.2
Special Instructions
If you have questions, please call 800-533-1037 (HLA customer service) for assistance in selecting the proper HLA test for the patient.
Limitations
This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Specimen Requirements
Specimen
Whole blood or buccal swabs
Volume
7 mL whole blood or four buccal swabs
Minimum Volume
3 mL whole blood or four buccal swabs
Container
Lavender-top (EDTA) tube or four buccal swabs in a sealed envelope (buccal swab kit). When submitting buccal swabs, please use a buccal swab kit provided by LabCorp. To obtain the buccal swab kit, please telephone 800-533-1037.
Storage Instructions
Maintain specimen at room temperature; protect from extreme heat or cold. Do not freeze.
Causes for Rejection
Hemolyzed specimen; clotted specimen; wrong specimen container
Footnotes
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 167331 | HLA-A (CWD) | 019414 | HLA-A | 13298-5 | ||
| 167331 | HLA-A (CWD) | 019415 | HLA-A | 13298-5 | ||
| 167331 | HLA-A (CWD) | 167384 | HLA Methodology | 49549-9 | ||
| Order Code | 167331 | |||||
| Order Code Name | HLA-A (CWD) | |||||
| Order Loinc | ||||||
| Result Code | 019414 | |||||
| Result Code Name | HLA-A | |||||
| UofM | ||||||
| Result LOINC | 13298-5 | |||||
| Order Code | 167331 | |||||
| Order Code Name | HLA-A (CWD) | |||||
| Order Loinc | ||||||
| Result Code | 019415 | |||||
| Result Code Name | HLA-A | |||||
| UofM | ||||||
| Result LOINC | 13298-5 | |||||
| Order Code | 167331 | |||||
| Order Code Name | HLA-A (CWD) | |||||
| Order Loinc | ||||||
| Result Code | 167384 | |||||
| Result Code Name | HLA Methodology | |||||
| UofM | ||||||
| Result LOINC | 49549-9 |