Test Details
Methodology
Presence of antibody bound to human lymphocytes detected by immunofluorescence and flow cytometry
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Detection of the presence of antidonor antibodies (HLA) in a potential solid organ recipient, T- and B-cell crossmatch detects the presence of anti-Class I/Class II HLA antibodies in the recipient that would recognize donor-specific antigens. The presence of antibodies against donor HLA antigens is a contraindication for transplantation and could lead to immediate/acute organ rejection.
Special Instructions
To schedule this test, you must telephone 800-533-1037, HLA customer service.
Limitations
Although the primary antibodies that are detected by this assay are to HLA, other non-HLA-specific antibodies could cause positive T- and/or B-cell crossmatch. This testing only demonstrates antibodies that recognize donor antigens and cannot distinguish clinical relevant antibodies (eg, complement binding ability) from nonclinical antibodies. This assay only detects the presence of IgG antidonor antibodies and does not detect IgM or other immunoglobulin subclasses.
This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Specimen Requirements
Specimen
Donor sample: Whole blood; Recipient sample: Serum
Volume
Donor sample: 12 mL; Recipient sample: 2 mL
Minimum Volume
Donor sample: 8 mL; Recipient sample: 2 mL
Container
Donor sample: Yellow-top (ACD) tube (solution A or B). Recipient sample: Red-top tube or gel-barrier tube
Storage Instructions
Maintain whole blood and serum at room temperature. Donor specimens should be analyzed within 72 hours of collection. Recipient serum may be stored at 4°C for one week or at -20°C indefinitely.
Patient Preparation
None
Causes for Rejection
EDTA or heparin tube; hemolysis; clotted specimen; donor samples exceeding 72 hours
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 167160 | Allo-Crossmatch by Flow | 167191 | AlloCrossmatch T-Cell(ALTCELL) | 48409-7 | ||
| 167160 | Allo-Crossmatch by Flow | 167192 | AlloCrossmatch B-Cell(ALBCELL) | 48390-9 | ||
| Order Code | 167160 | |||||
| Order Code Name | Allo-Crossmatch by Flow | |||||
| Order Loinc | ||||||
| Result Code | 167191 | |||||
| Result Code Name | AlloCrossmatch T-Cell(ALTCELL) | |||||
| UofM | ||||||
| Result LOINC | 48409-7 | |||||
| Order Code | 167160 | |||||
| Order Code Name | Allo-Crossmatch by Flow | |||||
| Order Loinc | ||||||
| Result Code | 167192 | |||||
| Result Code Name | AlloCrossmatch B-Cell(ALBCELL) | |||||
| UofM | ||||||
| Result LOINC | 48390-9 |