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Antinuclear Ab Profile, 11-biomarker Profile, by Multiplex Immunoassay, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B, Sm/RNP, Ribosomal P
CPT:
83516; 86225; 86235(x9)
Synonyms
- ANA Comprehensive Plus Profile
Test Includes
Centromere B; chromatin; dsDNA; Jo-1; ribosomal P; RNP; scl-70; Sjögren's A; Sjögren's B; Sm/RNP; Smith (Sm)
Special Instructions
Test is intended to be used as a follow-up to Antinuclear Antibodies (ANA) Direct [164855].
Expected Turnaround Time
2 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Documents
Specimen Requirements
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Storage Instructions
Room temperature
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination; icterus
Test Details
Use
Detect antibodies associated with SLE and mixed connective tissue disease. Sm antibodies are highly specific for SLE. RNP antibodies are found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells. Autoantibodies to the ribosomal P protein represent a highly specific marker for the diagnosis of SLE, where they have been associated with certain clinical manifestations. The native protein SmRNP contains two different regions: Sm and RNP that are different regions of the same macromolecule. SmRNP is the best antigen for detection of antibodies to either Sm or RNP but cannot discriminate between the antibody specificities. Inclusion of SmRNP increases sensitivity while inclusion of Sm and RNP increases specificity.
Methodology
Multiplex flow immunoassay
References
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LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 164920 | Antinuclear Ab 11 by Multiplex | 096343 | Anti-DNA (DS) Ab Qn | IU/mL | 5130-0 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 016354 | RNP Antibodies | AI | 29374-6 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 016362 | Smith Antibodies | AI | 11090-8 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 016376 | Smith/RNP Antibodies | AI | 27048-8 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 018707 | Antiscleroderma-70 Antibodies | AI | 27416-7 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012684 | Sjogren's Anti-SS-A | AI | 17792-3 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012692 | Sjogren's Anti-SS-B | AI | 17791-5 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012581 | Antichromatin Antibodies | AI | 51775-5 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012701 | Antiribosomal P Antibodies | AI | 13636-6 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 161457 | Anti-Jo-1 | AI | 11565-9 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 164816 | Anti-Centromere B Antibodies | AI | 53982-5 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 164879 | See below: | N/A |
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