Test Details
Methodology
Multiplex flow immunoassay
Result Turnaround Time
2 - 3 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Centromere B; chromatin; dsDNA; Jo-1; ribosomal P; RNP; scl-70; Sjögren's A; Sjögren's B; Sm/RNP; Smith (Sm)
Use
Detect antibodies associated with SLE and mixed connective tissue disease. Sm antibodies are highly specific for SLE. RNP antibodies are found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells. Autoantibodies to the ribosomal P protein represent a highly specific marker for the diagnosis of SLE, where they have been associated with certain clinical manifestations. The native protein SmRNP contains two different regions: Sm and RNP that are different regions of the same macromolecule. SmRNP is the best antigen for detection of antibodies to either Sm or RNP but cannot discriminate between the antibody specificities. Inclusion of SmRNP increases sensitivity while inclusion of Sm and RNP increases specificity.
Special Instructions
Test is intended to be used as a follow-up to Antinuclear Antibodies (ANA) Direct [164855].
References
Astion ML, Wener MH, Hutchinson K. Autoantibody testing: The use of immunofluorescence and other assays. Clin Lab News. 2000; (7):30-34.
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Eriksson C, Engstrand S, Sundqvist KG, Rantapää-Dahlqvist S. Autoantibody formation in patients with rheumatoid arthritis treated with anti-TNF alpha. Ann Rheum Dis. 2005 Mar; 64(3):403-407. 15297281
Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997 Sep; 43(9):1749-1756. 9299971
Golightly M, Golightly C. Laboratory diagnosis of autoimmune diseases. MLO Med Lab Obs. 2002 Jul; 34(7):12-16, 20. 12134551
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Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med. 2000 Jan; 124(1):71-81. 10629135
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Specimen Requirements
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 14 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Storage Instructions
Room temperature
Causes for Rejection
Hemolysis; lipemia; gross bacterial contamination; icterus
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 164920 | Antinuclear Ab 11 by Multiplex | 096343 | Anti-DNA (DS) Ab Qn | IU/mL | 5130-0 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 016354 | RNP Antibodies | AI | 29374-6 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 016362 | Smith Antibodies | AI | 11090-8 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 016376 | Smith/RNP Antibodies | AI | 27048-8 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 018707 | Antiscleroderma-70 Antibodies | AI | 27416-7 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012684 | Sjogren's Anti-SS-A | AI | 17792-3 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012692 | Sjogren's Anti-SS-B | AI | 17791-5 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012581 | Antichromatin Antibodies | AI | 51775-5 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 012701 | Antiribosomal P Antibodies | AI | 13636-6 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 161457 | Anti-Jo-1 | AI | 11565-9 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 164816 | Anti-Centromere B Antibodies | AI | 53982-5 | |
| 164920 | Antinuclear Ab 11 by Multiplex | 164879 | See below: | N/A | ||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 096343 | |||||
| Result Code Name | Anti-DNA (DS) Ab Qn | |||||
| UofM | IU/mL | |||||
| Result LOINC | 5130-0 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 016354 | |||||
| Result Code Name | RNP Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 29374-6 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 016362 | |||||
| Result Code Name | Smith Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 11090-8 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 016376 | |||||
| Result Code Name | Smith/RNP Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 27048-8 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 018707 | |||||
| Result Code Name | Antiscleroderma-70 Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 27416-7 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 012684 | |||||
| Result Code Name | Sjogren's Anti-SS-A | |||||
| UofM | AI | |||||
| Result LOINC | 17792-3 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 012692 | |||||
| Result Code Name | Sjogren's Anti-SS-B | |||||
| UofM | AI | |||||
| Result LOINC | 17791-5 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 012581 | |||||
| Result Code Name | Antichromatin Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 51775-5 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 012701 | |||||
| Result Code Name | Antiribosomal P Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 13636-6 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 161457 | |||||
| Result Code Name | Anti-Jo-1 | |||||
| UofM | AI | |||||
| Result LOINC | 11565-9 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 164816 | |||||
| Result Code Name | Anti-Centromere B Antibodies | |||||
| UofM | AI | |||||
| Result LOINC | 53982-5 | |||||
| Order Code | 164920 | |||||
| Order Code Name | Antinuclear Ab 11 by Multiplex | |||||
| Order Loinc | ||||||
| Result Code | 164879 | |||||
| Result Code Name | See below: | |||||
| UofM | ||||||
| Result LOINC | N/A |