Anaplasma phagocytophilum (HGE) Antibody, IgG and IgM, IFA

TEST: 164672
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CPT: 86666(x2)
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Synonyms

  • Anaplasma phagocytophilum
  • Ehrlichia
  • HGE
  • Tickborne

Expected Turnaround Time

1 - 4 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

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Specimen Requirements

Specimen

Serum

Volume

0.4 mL

Minimum Volume

0.2 mL

Container

Red-top tube or gel-barrier tube or serum transfer tube

Collection

Specimen should be free of bacterial contamination, hemolysis, and lipemia.

Storage Instructions

Refrigerate.

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination

Test Details

Use

This test is used for detection and semiquantitation of serum IgG and IgM antibodies to Anaplasma phagocytophilum (HGE) to aid in the diagnosis of human granulocytic ehrlichiosis.

Confirmation of diagnosis is based on laboratory testing, but antibiotic therapy should not be delayed in patients with a suggestive clinical presentation. The diagnosis of acute anaplasmosis cannot be confirmed solely by the presence of antibodies in a serum sample collected at a single timepoint. DNA PCR can be used to diagnose acute infection. Demonstration of a 4-fold rise in IgG-specific antibody titers by IFA in paired samples (acute and convalescent) confirms infection with A. phagocytophilum. IgM antibodies are less specific than IgG antibodies and are more likely to generate false positive results. IgM results alone should not be used for laboratory diagnosis. Antibody titers are frequently negative during the first 7-10 days of illness.

Limitations

The diagnosis of acute anaplasmosis cannot be confirmed solely by the presence of Anaplasma antibody in a serum sample collected at a single time point.

Methodology

Indirect fluorescent antibody (IFA)

Reference Interval

• IgG negative: <1:64

• IgM negative: <1:20

References

Centers for Disease Control and Prevention (CDC). Tickborne Diseases of the United States: A reference manual for healthcare providers. Sixth edition. 2022. CDC website: https://www.cdc.gov/ticks/tickbornediseases/index.html. Accessed May 2023.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
164672 Anaplasma phagocytophilum Ab 55150-7 164674 A. phagocytophilum IgG 23877-4
164672 Anaplasma phagocytophilum Ab 55150-7 164667 A. phagocytophilum IgM 23878-2
164672 Anaplasma phagocytophilum Ab 55150-7 164120 Result Comment: N/A

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